Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 3910 for “"fluorescence"”.
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Fluorescence Studies of Photosystem II Fluorescence Quenching During Desiccation
… coupled to PSII and characterized by a fast-fluorescence decay lifetime, and long-wavelength emission. The present study compares the relative ability of green algae and lichens to recover photosynthetic activity after periods of desiccation using steady state fluorescence emission …
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Development & Application of Fluorescence Spectroscopy & Fluorescence Lifetime Imaging to Biology & Medicine
… (FRET) and development and application of fluorescence lifetime-resolved imaging microscopy (FLIM). It includes work to extend the FRET and FLIM methods for studying microsecond reaction kinetics. The common thread and unifying theme of the dissertation is the development and application of …
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Helium resonance fluorescence LiDAR
… have made this possible. The helium resonance fluorescence LiDAR has the capability to probe the metastable helium content in the thermosphere and exosphere (within 250 km-750 km), where helium (23S) is most abundant. Strategies have been employed to increase the output power of the He Li- DAR …
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Fluorescence in reef corals
Fluorescence can be a powerful tool for probing biological systems. Prior measurements from Caribbean corals identified five fluorescing pigments in reef corals. In this thesis I study coral fluorescence spectra. I wanted to learn if fluorescence could be useful for large scale mapping and …
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Optimization and Applications of Fluorescence anisotropy assays and Fluorescence Resonance Energy Transfer Measurements
… mainly focuses on optimizing and applications of fluorescence anisotropy (FA) and FRET experiments for CaM-target interactions. First we evaluated the extent of interaction of fluorophores with CaM upon conjugation. In this study, three dyes were tested for influences of their charges on …
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Quantitative Fluorescence Studies in Living Cells: Extending Fluorescence Fluctuation Spectroscopy to Peripheral Membrane Proteins
… aims at addressing this challenge by extending fluorescence fluctuation spectroscopy (FFS) to simultaneously measure the oligomeric state of peripheral membrane proteins in the cytoplasm and at the plasma membrane. We developed a new method based on z-scan FFS that accounts for the fluorescence …
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Development &Amp; Application of Fluorescence Spectroscopy &Amp; Fluorescence Lifetime Imaging to Biology &Amp; Medicine
… (FRET) and development and application of fluorescence lifetime-resolved imaging microscopy (FLIM). It includes work to extend the FRET and FLIM methods for studying microsecond reaction kinetics. The common thread and unifying theme of the dissertation is the development and application of …
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Fluorescence lifetime distributions in proteins
… was developed to measure the natural fluorescence decay of single tryptophan residue proteins. The instrumentation uses mode-locked, synchronously pumped, cavity dumped and frequency doubled dye laser excitation. The detectors are modulated phase coherently with the mode-locked laser …
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Compressed fluorescence lifetime imaging microscope
Fluorescence lifetime imaging microscopy (FLIM) has been extensively applied in wide biomedical applications from single cell studies to medical diagnosis. While the state-of-the-art FLIM techniques are highly sensitive to biological dynamics such as fluorescence resonance emission transfer (FRET), …
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Resonance fluorescence in nitrogen dioxide.
Thesis: M.S., Massachusetts Institute of Technology, Department of Physics, 1971
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Nuclear resonance fluorescence in Si²⁸
… using a technique of nuclear resonance fluorescence. A silicon absorber was placed in a collimated beam of gamma rays from the reaction. The direction of collimation with reference to the direction of the bombarding protons governs the exact energy of the ground state games available for …
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Localized Excitation Fluorescence Imaging (LEFI)
… excitation of fluorescent cells, acquisition of fluorescence through the scaffold, and fluorescence mapping based on the position of the excitation light. To evaluate the capability and accuracy of the imaging system, human endothelial cells, stably expressing green fluorescent protein (GFP), …
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Determination of chlorine by fluorescence quenching
… for the determination of chlorine species. Fluorescence quenching to determine chlorine is a promising method because of its high sensitivity and selectivity. This study focuses on development of a sulfonamide-based collection system for low levels of chlorine in air. Quantitation of …
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Elucidating protein stabilization with fluorescence techniques
… of insulin via glycation.;In this thesis, fluorescence techniques have been used to monitor protein dynamics that are crucial to our understanding of protein aggregation and glycation. A synthetic protein that readily forms fibrous structures with homogenous growth kinetics is synthesized …
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Integrated optical fluorescence multi-sensor system
Research on fluorescence-based integrated optical immunoassay multisensing systems has gained growing interest in the last ten years. This is because the systems have the potential to simultaneously detect multiple analytes in a single measurement, and the techniques involved are fast, robust and …
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Resonance Fluorescence in a Photonic Crystal
… problems, that are related to the full resonance fluorescence problem: a pure dephasing model, the driven atom and resonance fluorescence in free space. In the lowest order of the system-environment coupling, the one-time expectation values for the full problem are calculated analytically and the …
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Resonance fluorescence of novel quantum emitters
Quantum dots (QD) emitting near the telecommunication O-band and excitons believed to be localized on defects in WSe2 monolayers are investigated using optical spectroscopy and resonance fluorescence (RF). The development of light sources emitting around 1300 nm is motivated primarily by the …
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PSF Sampling in Fluorescence Image Deconvolution
… an explicit volumetric representation of the fluorescence density in the sample and fit a neural network to the target z-stack to properly minimize a reconstruction cost function for an optimal result. Additionally, we do a weighted sampling of the point spread function to avoid unnecessary …
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Raman and fluorescence spectra of azabenzenes
… <p>In 1974, Brus and coworkers obtained a fluorescence spectrum of s-tetrazine from single-mode laser excitation. This spectrum was uniquely simple since the laser mode was so narrow (~0.0002 cm<sup>-1</sup>) that only 100 transitions in the Q-branch of the 6a<sup>1</sup><sub>0</sub> band …
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New Fluorescence Sensors for Critical Materials
… of minerals of significance using ‘novel’ fluorescence sensing through three regimes that are poorly covered in the existing literature: 1. Visible-light excitation and near infrared emission 2. “Cryogenic” fluorescence 3. Upconversion fluorescence Extending luminescence conditions into …
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