Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"fluo-4"”.
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Calcium Signaling Dynamics in RBL Cells Under Pulsed Ligand Exposure
… the cell signaling process, from immunofluorescence to single particle tracking. These techniques are advantageous in their own right, but having the ability to interrogate suspension and adherent cells with reagents at high temporal resolution and image them simultaneously is a process …
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Development of a flexible image-based approach for studying signal transduction in isolated arterioles
… (Oxonol, acceptor). For measurement of [Ca²+]i Fluo-4 AM (ex 488nm) was used. Initial studies examined toxicity of the multiple fluorophores. Isolated cells remained viable after loading with the fluorophores. Arterioles maintained myogenic, and agonist, responsiveness pre and post dye loading …
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Investigation of the mechanisms underlying the effects of hyperglycaemia on cardiac structural and electrical remodelling
… or high glucose conditions. A Ca2+ -sensitive fluorescent dye Fluo-4 was used to measure calcium transients and a voltage-sensitive dye di-4-ANEPPS was used to record action potentials. Cellular biomarkers were detected using immunocytochemistry, confocal microscopy, and Western blotting as …
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Re-evaluation of the in vitro activity of the protease activated receptor-1 (PAR1) pepducin PZ-128
… PAR1 expression and receptor activity using Fluo-4 AM calcium flux assays. MTT assay was used to assess cell viability, with significant cell death observed in PZ-128-treated cells following 100 μM exposure for 24 hours, which coincided with loss of pAkt.;Interestingly, PZ-128 (25-100 μM), …
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HUMAN CLCA2 MODULATES THE CONDUCTANCE OF CALCIUM-ACTIVATED CHLORIDE CHANNELS BY REGULATION OF INTRACELLULAR CALCIUM
… increase intracellular calcium levels. With Fluo-4 dye calcium imaging, we found that CLCA2 expression enhanced both ER calcium stores and SOCE upon exhaustion of intracellular stores, and the SOCE response could be abolished by a specific inhibitor of SOCE, BTP-2. This inhibitor also …
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PIP2 Modulation of Afterhyperpolarizations in Magnocellular Supraoptic Neurons
… enhancement, we changed the Ca2+ indicator to fluo-4 and was able to observe enhancement under specific conditions. This suggests that VP modulation is critically dependent on the time course and buffering of available Ca2+. </p> <p>Finally, I also performed a cursory evaluation of possible …
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Investigation of the role of Staphylococcus Aureus toxins in a cartilage explant model of septic arthritis
… arthritis was developed in this study. Utilising fluorescence-mode confocal laser scanning microscopy (CLSM), the model, which avoided the complexities of a host immune response, permitted an assessment of the following: (1) the spatial and temporal quantification of in situ chondrocyte viability …
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Heterogenous Change in Neuronal Bursts Following Recovery from Activity Silencing
Silencing of activity in hippocampal neuronal cultures was used to study how dynamic neuronal activity achieves a state of homeostasis, using calcium imaging to detect neuronal firing patterns. Recovering cultures were found to display abnormal activity patterns after 48hrs of exposure to …