Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"flowcytometry"”.
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Somatic Mutation Detection in Leukemia-Derived Circulating DNA: Utility in Monitoring Clonal Dynamics and Disease Response in Pediatric Acute Lymphoblastic Leukemia
… time points as well as, to detect mutations in Flowcytometry (FC) MRD-negative patients. Taken together, sequential analysis of Ct-DNA in plasma demonstrates a role, as a non-invasive technique, for detecting the clonal composition as well as, tracking clonal dynamics in pediatric ALL.</p>
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Mechanistic studies on aziridinylbenzoquinones and CCNU [l-(2-chloroethyl)- 3-cyclohexyl-l-nitrosourea] in cultured tumour cells
… It was found using HPLC, Cytotoxicity, comet and flowcytometry studies that these two quinones behave very similarly in that their cell killingpotencies are enhanced by the presence of the reducing enzyme DT-Diaphorase in thecells. However we have shown that Es5 has a much higher partition …
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Synthesis and evaluation of novel heterocyclic compounds as anticancer agents
… and A204 mammaliancell lines by MTT assay, and flowcytometry. 3-Hydroxy-4-methoxy-N-(2-((3,4,5-trimethoxybenzyl)amino)pyridin-3-yl)benzenesulfonamide, was the most potentcompound evaluated with an IC50 of 1.15µM in the Carcinoma A549 cell line. It is alsocaused cells to hold up in the G2/M phase …
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Investigation of neuron T cell interaction in central nervous system tuberculosis
… surface markers and cytokines induced by Mtb. In flowcytometry and ELISA analyses, infection exhibited a robust inflammatory response with increased neuronal production of cytokines such as IL1β, IL6, TNF and regulatory cytokine IL10 in vitro and in vivo. Neuronal MHC class I expression was …
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Investigating the protective role of the natural hormone Melatonin, in reducing drug-induced cardiotoxicity in the therapy of chronic diseases
… metabolic changes were ameliorated by melatonin. Flowcytometry and Alamar Blue staining methods demonstrated that DOX robustly induced apoptosis in H9c2 cells (~30%) which was reversed by melatonin. Doxorubicin-induced stress in H9c2 cells dramatically altered gene expression in several key …
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The effects of herbal extracts and compounds on the glucose metabolism in Hep G2 cells
… analysis. In studies on glucose uptake using flowcytometry, metformin increases 1.8-fold, goldenseal stimulates 2-fold, and berberine augments 3.4-fold more glucose uptake than the control (p<0.001). In glucose transporter-1 expression assays, goldenseal and berberine increased the expression …
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Evaluation of inhibitors of histone deacetylases as potential neurotrophic agents
… role of cAMP, and cell- cycle analysis with flowcytometry were also studied. </p> <p>Vorinostat, a pan HDAC inhibitor, independently induced significant neurite outgrowth in NS-1 cells, similar to that of NGF. The inhibitors of MEK1/2 & PI3K attenuated the NGF and vorinostat mediated neurite …
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Promoter Polymorphisms of Cysteinyl Leukotriene Receptor 1 (CysLTR1) Influence the Effect of Leukotriene Modifier on Transcriptional Regulation of the CysLTR1 Gene Induced by IL-4
… cell line, A549 cells, by real time PCR and flowcytometry. Effect of MK-571 on the transcriptional induction of CysLTR1 by IL-4 was examined by luciferase reporter assay. Results: In the screening of 5？ upstream region of CysLTR1 gene in 43 normal healthy controls, three single nucleotide …
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B-cell development and function in the absence of the spleen
Splenectomized and asplenic individuals are prone to infections with encapsulated bacteria, mainly caused by Streptococcus pneumoniae. The function of the spleen in the defense against this pathogen is unknown. We used congenitally asplenic Hox11-/- mice to study the function of the spleen for the …
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TARGETING THE DNA DAMAGE RESPONSE PATHWAY FOR THE TREATMENT OF AGGRESSIVE THYROID CANCERS, IN VITRO STUDIES
… either alone or in combination with DX or LENV. Flowcytometry analysis was used to evaluate cell cycle variations upon single and combined treatments. Results. A significant association between resistance to TKI treatments and the presence of TP53 inactivating mutations was found in a cohort of …
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Validierung der Zellzyklusdiagnostik bei Ataxia telangiectasia
Im Rahmen der vorliegenden Arbeit wurde die Methode der durchflusszytometrischen Zellzyklusanalyse von Lymphozyten bei Patienten mit der klinischen Verdachtsdiagnose Ataxia telangiectasia beschrieben. Hierzu wurden die Daten von 327 Patienten ausgewertet. In 82 Fällen ergab sich eine Bestätigung …
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Funktionelle und molekulare Pränataldiagnostik der Fanconi-Anämie
Die Zellzyklusanalyse an kultivierten Fruchtwasserzellen zur pränatalen Diagnostik der Fanconi-Anämie ist nicht hinreichend zuverlässig und sollte aufgrund der teilweisen Verfälschung des Ergebnisses durch tetraploide Zellen und unzureichende Mitogenantwort sowie eventuell einen hohen Anteil …
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Proteinase 3 and Neutrophil Apoptosis in ANCA-Associated Systemic Vasculitis
ANCA-Associated Systemic Vasculitis (AASV) is characterized by leukocytoclasis- dying neutrophils surrounding small blood vessels. Previous studies have shown increased plasma PR3 and increased proportion of neutrophils expressing plasma membrane-associated PR3 (mPR3+). Neutrophils were isolated by …