Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 33 for “"flow cytometer"”.
-
Development of the in vivo flow cytometer
An in vivo flow cytometer has been developed that allows the real-time detection and quantification of circulating cells containing fluorescent proteins or labeled with fluorochrome molecules in live animals, without the need to extract blood samples. A stationary laser beam is focused by a …
-
Optimization of the mini-flo flow cytometer dc by Mukund C. Venkatesh.
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 1996.
-
Modular Neural Networks for Analysis of Flow Cytometry Data
… developed an automated optical tool, called a Flow Cytometer (FC), to analyse samples quickly and in large quantities. The flow cytometer has<br/>successfully been applied to real phytoplankton studies. However, analysis of the data extracted from samples is still required. Artificial Neural …
-
Investigation of the genotoxic potential of the marine biotoxins okadaic acid and azaspiracids
… and assess the right concentration range. Flow cytometer analysis was included to detect the possible involvement of apoptotic processes. In house background data for all endpoints were established using positive controls. Three different cell lines, Jurkat T cells, CaCo-2 cells and HepG-2 …
-
Applied Plankton Image Classification for Imaging FlowCytobot Data
… accessibility for future studies. The Imaging FlowCytobot (IFCB), an automated submersible flow cytometer, produces high resolution images of plankton at rates up to 10 Hz for months or years, resulting in billions of images. This project compares various methods to categorize incoming images …
-
Altering Nitric Oxide Bioavailability and Lipid Profiles in Endothelial Cells By Polycyclic Aromatic Hydrocarbons in Particulate Matter
… measured using the fluorometric method with a flow cytometer. Lipid peroxidation was assessed based on the formation of malondialdehyde. Nitric oxide synthase was calculated using a cell-permeable diacetate that reacts with NO to form a fluorescent triazolotluorescein. Then, lipids of HCAEC …
-
Scalable development of multiplexed microparticle technologies for optical single-cell barcoding
… a modified microscope and a custom-developed flow cytometer. We expect that the proposed technology offers a platform to identify single cells in various single-cell measurements and allows the acquired data of same cells to be integrated using the optical barcodes.
-
NON-CONVENTIONAL YEASTS AS CELL FACTORIES: FROM FOOD WASTE TO ADDED-VALUE PRODUCTS.
… to save freshwater. In this work, an analytical flow cytometer method was developed for a rapid and sustainable monitoring of lipid accumulation. - Expressing a heterologous adhesin protein (CFL1p) to enhance the flocculating ability of C. oleaginosum ATCC 20509. This reduced the time required …
-
A comparison of flow cytometry and conventional microbiology in the study of biofilms.
A comprehensive study on the application of flow cytometry (FCM) for the analysis of biofilms has been undertaken and the results presented in this thesis have shown that flow cytometry can been successfully used to enumerate, sort and image the bacteria and amoebae in biofilms and water …
-
Spatiotemporal modeling and model restructuration approaches in studies of intracellular signalling pathways
… study carried out using confocal microscopy and flow cytometer. To understand the particle uptake behavior with time and steady state condition, reaction-diffusion and kinetics model has been developed in these work. After a detailed analysis of experimental data and models, it showed that total …
-
Herstellung und Charakterisierung monoklonaler Antikörper gegen equine Leukozyten
… 5-50 are binding specifically to equine T-cells. Flow cytometer analyses demonstrated an increased frequency following the stimulation with Concanavalin A. The frequency of mAb 4-13- and mAb 4-58-positive cells matched the total frequency of CD4- and CD8-positive cells. The number of cells, marked …
-
The effects of magnetic field (MFs) on in vitro culture of human umbilical cord derived mesenchymal stem cells (hUC-MSCs)
… when compared to IE and NC groups. In addition, flow cytometer analysis discovered no significant difference in the expression of the surface markers of DE group compared to IE and NC. SMF also was observed to have the potential to induce higher expression of pluripotency-associated markers …
-
Accelerating antimalarial drug discovery through repositioning
… by the preliminary screen. The SYBR Green flow cytometer method, capable of providing direct parasitaemia estimates and stage-specific information, was used for second-phase characterisation of the hits. From these, antiamoebic compound emetine dihydrochloride hydrate was identified as a …
-
High throughput 3D optical microscopy : from image cytometry to endomicroscopy
… a high throughput depth resolved widefield image cytometer based on the structured light illumination and the high speed remote depth scanning. This system improves imaging throughput by an order of magnitude over the current technology and can potentially be applied to image cytometry …
-
Population dynamics and diversity of Synechococcus on the New England Shelf
… cells (hours-days). Development of an automated flow cytometer (FlowCytobot) has enabled hourly observation of Synechococcus at the Martha’s Vineyard Coastal Observatory (MVCO) since 2003. In order to ascribe changes in cell abundances to either growth or loss processes, information on division …
-
Kinetik von NK-Zellen und gamma-delta-T-Zellen nach Infektion von Rhesusaffen mit Immundefizienzviren
… Samples were measured on a multicolour flow cytometer and subsequently twelve parameter analyses, including size and inner complexity characteristics, were performed. Changes in the frequency of NK cells and their differentiation were detected, after definition as lineage marker negative …
-
Population dynamics and diversity of Synechococcus on the New England shelf
… cells (hours-days). Development of an automated flow cytometer (FlowCytobot) has enabled hourly observation of Synechococcus at the Martha's Vineyard Coastal Observatory (MVCO) since 2003. In order to ascribe changes in cell abundances to either growth or loss processes, information on division …
-
Microfluidic Engineering for Ultrasensitive Molecular Analysis of cells
… translocation of native proteins using a common flow cytometer which detects fluorescence intensity without imaging. Using our approach, we successfully detected the translocation of native NF-kappa B (an important transcription factor) at its native expression level and examine the temporal …
-
Development and application of a high throughput cell based assay to identify apoptosis inducing proteins, and functional characterization of the candidate Vacuole Membrane Protein 1 (Vmp1)
… automated and data acquisition was done using a flow cytometer with an integrated 96-well plate reader. A total of 200 proteins have been screened in the assay, out of which five were identified to be significant activators of apoptosis. One of the candidates, Vacuole Membrane Protein 1 (Vmp1), …
-
New Instrumentation and Methods for Analyzing Biological Compounds for Drug Discovery and Discovering Novel Disease Markers
… binding to 5-HT1A receptors was monitored using flow cytometry. Liposome shell microparticles may also be used in packed bed membrane-based liquid chromatography. To retain the particles a frit is needed within the column. In this work a new thermal polymerization method for fabrication of …
Page 1 of 2