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Showing 1 to 4 of 4 for “"flexizyme"”.
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Ribosomal Synthesis of N-methylated peptides
… synthesis using recombinant elements (PURE) and Flexizyme were used in order to reassign specific codons to N-methyl amino acids. mRNA-dependent translation system enable us to make our desired peptides with N-methyl amino acids. This technology is a convenient tool for the construction of …
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Characterizing peptide-modifying enzyme activity in RiPP biosynthesis
… aminoacyl-tRNA ligase (PEARL) enzyme. Using flexizyme-generated aminoacyl-tRNAs, both the amino acid and tRNA body were shown to jointly encode specificity while still permitting incorporation of noncanonical amino, hydroxy, and thiocarboxylic acids.
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A General Platform for Ribosome Engineering
… how the blank codons can be reassigned using the flexizyme system. Chapter 7 concludes the thesis with a description of ongoing work to use HiSTRIVE to discover ribosome variants with altered polymerization properties under a reprogrammed genetic code. In par- ticular, β-amino acids are an …
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Isomerization-Locked Alkene Analogues of Xaa–Pro Dipeptides in the Proteins Collagen and Bora
… for enzymatic aminoacylation using the dFx flexizyme, an RNA enzyme. The native dipeptide was successfully coupled to tRNACUA and is ready for incorporation into a full-length Bora protein by in vitro transcription-translation. Both cis- and trans-locked alkene mimics have been converted to …