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Showing 1 to 16 of 16 for “"flavodoxin"”.
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Characterization of ferredoxin and flavodoxin as molecular indicators of iron limitation in marine eukaryotic phytoplankton
Expression and regulation of the ferredoxin and flavodoxin proteins in marine phytoplankton were investigated to assess their utility as biomarkers of iron limitation. A phylogenetic survey of seventeen microalgal species showed flavodoxin induction, with accompanying ferredoxin repression, to be a …
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Characterization of ferredoxin and flavodoxin as molecular indicators of iron limitation in marine eukaryotic phytoplankton
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1997.
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Effects of macromolecular crowding and small ions on the folding, structure, and stability of Desulfovibrio desufuricans flavodoxin
… a model protein -- Desulfovibrio desulfuricans flavodoxin. Flavodoxin is a small (15.7 kDa), single domain, cytoplasmic protein with alpha-helical and parallel beta-sheet secondary structural elements arranged in one of the five most common protein folds (the flavodoxin-like fold). Using a range …
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Engineering protein components for living electronics
… I describe my efforts to engineer ferredoxin and flavodoxin electron transfer proteins as components in controlling electron flow in cells. In these studies, I probe the tolerance of a cyanobacterial flavodoxin to insertion of a small octapeptide, which elongates the primary structure. I find that …
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Genetic analysis of nifF and nifA and site-directed mutagenesis of nifE in Azotobacter vinelandii
… sequence of the nifF gene, which encodes a flavodoxin, was determined. Specific mutation strains indicated that in <i>A vinelandii</i> flavodoxin is not the unique physiological electron donor to nitrogenase. The nifF gene appears to be constitutively expressed but under nitrogen fixing …
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Studies on nucleotide levels and electron transport genes of Clostridium acetobutylicum P262
… and database searches showed good similarity to flavodoxin proteins from many organisms. Based on alignments to the amino acid sequences of these flavodoxins, as well as the fact that Chen and Blanchard (1979) reported that reduced flavodoxin can transfer electrons to metronidazole, the sequence …
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Selective extraction of colorful proteins using reverse micelles
… EGFP, <em>Cyanobacterium</em> <em>anabaena</em> flavodoxin, and DsRed2 from reverse micellar systems. Experiments were also conducted using crude lysate and protein mixtures. Ultimately more research needs to be conducted with reverse micelles for it to become an accepted bioseparation technique, …
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The relationship between iron and nitrogen fixation in Trichodesmium spp.
… Fe stress response gene isiB, which encodes for flavodoxin a non-Fe containing substitute for ferredoxin. I determined that high isiB gene expression corresponded to cell-specific reductions in N fixation rates in both phylogenetic clades of Trichodesmium grown on varying levels of Fe. Using …
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The relationship between iron and nitrogen fixation in Trichodesmium spp.
… Fe stress response gene isiB, which encodes for flavodoxin a non-Fe containing substitute for ferredoxin. I determined that high isiB gene expression corresponded to cell-specific reductions in N fixation rates in both phylogenetic clades of Trichodesmium grown on varying levels of Fe. Using …
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Photochemistry, photophysics and spectroscopy of redox states of flavins relevant to photoactive flavoproteins
… states have been stabilized in the protein: flavodoxin and glucose oxidase. Ultrafast transient absorption measurements were performed in the visible and mid infrared region in order to characterize the excited and ground state‟s dynamics and vibrational spectra, and to probe the effect of …
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Development of a Model for the Kinetics and Mechanism of Nitrogenase
… S = 0 state by the <em>in vivo</em> reductant flavodoxin; and a novel account of salt effects that weaken the nitrogenase complex to increase the rate of complex dissociation, the rate-limiting step in nitrogenase catalysis.</p>
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Structural studies of S-adenosyl-L-methionine radical enzymes involved in tRNA and natural product biosynthesis
… influence binding of the biological reductant Flavodoxin. This variance in the core AdoMet radical fold emphasizes the structural diversity of this superfamily. On the other hand, we see conservation of an overall three-domain architecture for the maturation of ribosomally synthesized and …
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Mechanism of biosynthesis of the dimanganese-tyrosyl radical cofactor of class lb Ribonucleotide reductase
… in class lb RNR systems. We show that NrdI is a flavodoxin-like protein with unusual redox properties. Although Mnr 2-NrdF does not react with 02, in the presence of reduced NrdI (Nrdlhq) and 02, it assembles an active dimanganese(III)-Y· (Mn 12- Y·) cofactor. Biochemical evidence indicates that …
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Biodegradation of RDX in Rhodococcus spp.
… degrading genes, xplA and xplB encoding a novel flavodoxin fused cytochrome P450 and its reductase partner respectively, were first identified in R. rhodochrous 11Y. This unusual P450 system has now been found in almost all of the RDX degrading bacteria isolated so far. To date, XplA/B remains …
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Understanding the Effects of Higher-Order Assembly on the Structure of Sulfite Reductase
… composed of the following domains: a FMN binding flavodoxin (Fld)-like-domain, a connection domain interrupting FAD/NADPH bind- ing FNR (Ferredoxin/NADP+ reductase)-like-domain and a flexible linker connecting Fld and FNR domains. This flexible linker is ∼ 30 amino acids long and remains crucial …
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An exploratory study of Helicobacter suis control strategies
… including homologs for mviN, the H. pylori flavodoxin gene (fldA) and the H. pylori high-temperature requirement A gene (htrA). Sequences coding for outer membrane proteins involved in adhesion to gastric cells, such as H. pylori adhesin A (HpaA), HorB and some Hof members were present in H. …