Global ETD Search
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Showing 1 to 20 of 46 for “"flaviviruses"”.
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Human Antibody Response to Flaviviruses and SARS-Coronavirus-2
… focuses on encephalitis-causing tick-borne flaviviruses, specifically TBEV and POWV. Both TBEV and POWV are emerging human pathogens that cause potentially fatal disease; there are no disease-specific treatments. Mouse monoclonal antibodies are protective against TBEV and POWV, but little is …
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Methods for the Genetic Stabilization of Reporter Flaviviruses and their Applications
Infectious reporter flaviviruses have been reported since 2003, and they have the potential to be utilized in diverse scientific areas such as investigating viral pathology, conducting high-throughput drug screens, running rapid serum neutralization tests, and diagnosing disease. Despite the value …
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The molecular basis for evasion of antibody-mediated neutralization in flaviviruses
In order to establish infection, flaviviruses induce mutations in antigenic proteins as a strategy to evade neutralizing antibodies; a phenomenon known as antibody-mediated neutralization resistance. Most of the critical mutations associated with antibody resistance are located in the envelope …
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Methods for the Genetic Stabilization of Reporter Flaviviruses and their Applications
Infectious reporter flaviviruses have been reported since 2003, and they have the potential to be utilized in diverse scientific areas such as investigating viral pathology, conducting high-throughput drug screens, running rapid serum neutralization tests, and diagnosing disease. Despite the value …
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Characterization of Host Factors and Anti-viral Compounds for Diverse Mosquito-borne Flaviviruses
… a common mechanism to promote replication of flaviviruses. We report that DENV, ZIKV, and yellow fever virus (YFV) infections were impressively inhibited, while West Nile Virus (WNV) infection was unchanged, in cell lines engineered to lack EMC subunit 4 (EMC4). Furthermore, targeted depletion …
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Assessing and understanding the generation and function of RNA decay intermediates in non-insect borne flaviviruses
Cellular gene expression is an intricate process regulated on many levels that allows the cell to react correctly to stimuli or to maintain homeostasis. RNA viruses must act to preferentially drive production of their own messenger RNAs (mRNAs) and proteins in order to successfully replicate and …
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Characterization of the Antiviral Effector IFI6
… which establish an antiviral state in the cell. Flaviviruses such as dengue virus, Zika virus, and West Nile virus rely intimately on host pathways for completing a replication cycle, and have developed strategies to overcome the inhibitory effect of the innate immune response. To identify host …
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An Examination of the Safety and Efficacy of Aripo-Zika as a Zika Virus Vaccine Candidate
Flaviviruses are a genus of vector-transmitted viruses that are nearly globally distributed, and flavivirus infections can result in life threatening diseases. Many flaviviruses such as Dengue, West Nile, yellow fever and Zika viruses are globally distributed. Zika virus (ZIKV) is a single strand …
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Phenotypic Characterization of Mutations Made to the Envelope Protein of West Nile Virus
… sequela. The envelope (E) protein of flaviviruses has three domains and is the major component of the virion surface, with the EIII being the putative receptor binding domain. Previous studies showed that Gly to Ala mutation of EIII residue 331 (G331A) yielded a virus that had altered …
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Development, Characterization, and Use of Molecular Tools to Study Immune-Driven Zika Virus Evolution
… group of emerging pathogens of interest is flaviviruses. Flaviviruses are arthropod-borne viruses (arbovirus) in the family Flaviviridae. The medically relevant flaviviruses can be divided into two groups – tick-borne and mosquito-borne. Included within the mosquito-borne flaviviruses group …
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Characterization of epitope-blocking ELISA for differential diagnoses of secondary flavivirus infections
… currently complicated in areas where multiple flaviviruses circulate. To address this complication, the dissertation project investigated the ability of WNV-specific monoclonal antibodies to compete actively in binding the epitopes on the NS1 peptides and to distinguish between antibodies …
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Battle of the Viruses: Aripo Virus Induced Superinfection Exclusion of Mosquito-borne Viruses
Flaviviruses are a single-stranded, positive sense, RNA virus that affect around 400 million people annually. Flaviviruses are transmitted by arthropod vectors, the most common vector being the mosquito. Currently, many mosquito control strategies are in use, these control strategies are diverse in …
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Site-Specific Labelling of the Flavivirus Envelope Protein using Genetic Code Expansion
Flaviviruses are arthropod-borne viruses that constitute a significant global public health burden. The envelope protein (E) orchestrates the processes of virus assembly, budding, and entry into cells and is the primary target of neutralising antibodies. The E proteins arranged on the surface of …
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Generation and Characterization of Flavivirus Receptor-Binding Domain Mutants and Chimeras
Flaviviruses are positive-sense, single-stranded RNA viruses responsible for millions of infections annually. Primarily transmitted by ticks and mosquitoes, their associated diseases range from mild febrile infections to encephalitis and hemorrhagic fever. The molecular basis of the differences in …
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Translation of Partially Decayed Messenger RNAs in Yeast
<p>Flaviviruses are positive-strand single-stranded RNA viruses that are known to form pseudo-knot RNA structures that halt the progression of 5’→3’ exonuclease Xrn1. We show that these viral Xrn1-resistant structures (xrRNAs) can be used to protect specific homologously-expressed messenger RNAs …
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Evaluation of arthropod-borne virus recombination potential and phenotype: In vitro and in vivo analysis of flavivirus and alphavirus model systems
… analyses of naturally occurring recombinant flaviviruses have raised concerns regarding the potential for the emergence of virulent recombinants either post-vaccination or following co-infection with two distinct wild-type viruses. In order to characterize the conditions and sequences that …
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Investigating the role of the West Nile virus and Koutango virus envelope protein in virulence phenotypes using structural protein chimeras
Flaviviruses have caused, and continue to cause, both sporadic and regular outbreaks globally which result in significant global disease burden. To better understand how different portions of the virus contribute to transmission and disease caused by these viruses, researchers have found ways to …
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Correlated long-distance dynamics modulate monoclonal antibody binding resistance in flaviviral envelope pretein domain-3: A molecular dynamics simulations study
… to the envelope protein domain-3 (ED3) of flaviviruses. Previously it was shown that regions constituting antibody binding sites of dengue-2 (DEN2) and West Nile virus (WNV) ED3 were energetically coupled with the interior of the protein. Protein-protein interactions are characterized by …
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The Evolution of the Viral RNA Sensor OAS1 in Old World Monkeys and Cetartiodactyls
… in animal susceptibility to pathogens including flaviviruses such as dengue, West Nile, and hepatitis c virus. This thesis includes a population level analysis of OAS1 diversity within macaque and baboon species followed by a broader survey of the gene in nineteen Old World monkeys. My research …
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Role of <i>Ixodes scapularis</i> Sphingomyelinase-Like Protein (IsSMase) in Tick Pathogen Interactions
… is very similar to other medically important flaviviruses such as Tick-Borne Encephalitis virus (TBEV) and Powassan virus (POWV). Sphingomyelinase-like protein (IsSMase, a Sphingomyelinase D or SMase D, a venomous protein ortholog of spiders) is an enzyme present in ticks that helps to …
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