Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 81 for “"flavivirus"”.
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Using the single-cycle flavivirus particle replivax to study flavivirus replication and immune response
RepliVAX is a novel single-cycle flavivirus (SCFV) vaccine platform, which contains a deletion in the capsid (C) gene of West Nile virus (WNV) that prevents the formation of infectious particles, unless the C gene is trans-complemented by a WNV C-expressing cell line. RepliVAX immunization results …
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Characterization of Flavivirus-Infected Neurons
Powassan virus (POWV) is a tick-borne flavivirus (TBFV) that can cause severe encephalitis in humans with a case fatality rate as high as 15%. Patients who survived severe encephalitic disease can develop long-term sequelae that can be debilitating and life-long. In this dissertation, a primary …
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Host and viral determinants of flavivirus emergence and pathogenesis
… transmission capacity of an under-characterized flavivirus, Spondweni virus (SPONV). I show that SPONV causes fetal harm comparable to Zika virus (ZIKV) in a mouse model of flavivirus pathogenesis during pregnancy and that SPONV can be transmitted by Aedes aegypti mosquitoes. In Chapter 3, I …
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Characterization of single-cycle flavivirus particles for use as a vaccine to prevent West Nile disease and to examine immune responses to flavivirus infection
… we have developed RepliVAX WN, a single-cycle flavivirus (SCFV)-based vaccine to prevent West Nile disease. RepliVAX WN contains a C-deleted WNV genome, and is produced in trans-complementing cell lines that express WNV C. When used for vaccination, RepliVAX WN infects a single cell where the …
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Yellow Fever: Role of Heterologous Flavivirus Immunity on Urban Emergence
… the hypothesis that pre-existing, heterologous flavivirus immunity, specifically from dengue (DENV) and Zika (ZIKV) viruses, limits YFV viremia and transmission by Ae. aegypti. We infected cynomolgus macaques with DENV or ZIKV, then challenged them 6-9 months later with YFV. We then measured …
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Generation and Characterization of Flavivirus Receptor-Binding Domain Mutants and Chimeras
Flaviviruses are positive-sense, single-stranded RNA viruses responsible for millions of infections annually. Primarily transmitted by ticks and mosquitoes, their associated diseases range from mild febrile infections to encephalitis and hemorrhagic fever. The molecular basis of the differences in …
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Developing a Novel, Safe, and Effective Platform for Generating Flavivirus Vaccines
Viruses in the Flavivirus genus (e.g., Zika, yellow fever, dengue, West Nile, and Japanese encephalitis viruses) are arthropod-borne, globally distributed, and can cause a range of neurological or hemorrhagic diseases. The ongoing epidemics of flaviviral disease consistently demonstrate the need …
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Characterization of epitope-blocking ELISA for differential diagnoses of secondary flavivirus infections
… currently complicated in areas where multiple flaviviruses circulate. To address this complication, the dissertation project investigated the ability of WNV-specific monoclonal antibodies to compete actively in binding the epitopes on the NS1 peptides and to distinguish between antibodies …
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Site-Specific Labelling of the Flavivirus Envelope Protein using Genetic Code Expansion
Flaviviruses are arthropod-borne viruses that constitute a significant global public health burden. The envelope protein (E) orchestrates the processes of virus assembly, budding, and entry into cells and is the primary target of neutralising antibodies. The E proteins arranged on the surface of …
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Modeling host responses to tick feeding and tick-borne flavivirus evolution and dispersal
… In a final study, a model of tick-borne flavivirus evolution and dispersal was refined. This study suggests that Powassan encephalitis virus has been in North America for about 12,000 years, and that it will become a more significant public health risk as deer and tick populations …
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TICK-FLAVIVIRUS INTERACTIONS: DISCOVERY OF HOST PROTEINS THAT ARE AFFECTED IN VIRUS INFECTION
… M. Ph.D., Purdue University, August 2015. Tick-Flavivirus Interactions: Discovery of Host Proteins That Are Affected in Virus Infection. Major Professors: Catherine A. Hill and Richard J. Kuhn.
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Ribosomal Proteins RPLP1 and RPLP2 are Host Factors Critically Required for Flavivirus Infectivity by Promoting Efficient Viral Translation Elongation.
<p>The Flavivirus genus contains several arthropod-borne viruses that pose global health threats, including dengue virus (DENV). We identified two ribosomal proteins, RPLP1 and RPLP2 (RPLP1/2), that are crucial host factors required for translation of flaviviruses and efficient flavivirus infection …
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Evaluation of arthropod-borne virus recombination potential and phenotype: In vitro and in vivo analysis of flavivirus and alphavirus model systems
… analyses of naturally occurring recombinant flaviviruses have raised concerns regarding the potential for the emergence of virulent recombinants either post-vaccination or following co-infection with two distinct wild-type viruses. In order to characterize the conditions and sequences that …
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West Nile virus vaccination protects against Usutu virus disease in mice
… endemic in the Americas. Similar to other flaviviruses, USUV and WNV are maintained in a mosquito vector-avian host transmission cycle, with spillover infection into humans. Human infections of WNV and USUV are usually asymptomatic, but in severe cases can cause neuroinvasive disease. WNV …
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An Examination of the Safety and Efficacy of Aripo-Zika as a Zika Virus Vaccine Candidate
Flaviviruses are a genus of vector-transmitted viruses that are nearly globally distributed, and flavivirus infections can result in life threatening diseases. Many flaviviruses such as Dengue, West Nile, yellow fever and Zika viruses are globally distributed. Zika virus (ZIKV) is a single strand …
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Isolation and characterization of dengue virus membrane-associated replication complexes from Aedes aegypti
Ultrastructural studies of flavivirus replication have long observed proliferation of host membranes. Membrane-bound replication compartments have recently been isolated and characterized from flavivirus-infected mammalian cells, providing insight into the morphology, organelle of origin, and …
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Identification of Receptor Transporting Proteins As Conserved Antiviral Effectors in Vertebrates
… the bat Pteropus alecto as a potent inhibitor of flavivirus infection. Mechanistic studies determined that RTP4 is an RNA-binding protein that associates with flavivirus replication machinery, binds replicating viral RNA, and suppresses viral genome amplification. Phylogenomic analysis revealed …
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Characterization of the Antiviral Effector IFI6
… which establish an antiviral state in the cell. Flaviviruses such as dengue virus, Zika virus, and West Nile virus rely intimately on host pathways for completing a replication cycle, and have developed strategies to overcome the inhibitory effect of the innate immune response. To identify host …
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Mechanistic analysis of the Zika virus translation-replication switch
… is a capped, positive-sense RNA virus of the Flavivirus genus, which contains several notable human pathogens including Dengue virus (DENV) and Japanese encephalitis virus. Infection with ZIKV has been linked to congenital microcephaly as well as Guillain-Barré syndrome in infected adults. …
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The Structural Basis of Flaviviridae Interaction with Antibodies and Receptors
… and St. Louis Encephalitis Virus. Viruses of the Flavivirus genus within Flaviviridae are grouped into serocomplexes with similar clinical manifestations that are defined by cross-neutralization tests with polysera from heterologous infections. Japanese Encephalitis Virus: JEV) is the leading …
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