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Showing 1 to 12 of 12 for “"flagellar motor"”.
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STRUCTURAL AND BIOCHEMICAL CHARACTERIZATION OF THE BACTERIAL FLAGELLAR MOTOR HOOK COMPLEX AND THE MS:C-RING INTERFACE
The bacterial flagellar motor (BFM) is a highly complex and specialized nano-machine that powers the rotation of the flagellar filament. The flagellar filaments serve as propellers to push the cell forward and make movement in liquid and semi-solid surfaces possible. The BFM and associated hook and …
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The little engine that could: Characterization of noncanonical components in the speed-variable flagellar motor of the symbiotic soil bacterium Sinorhizobium meliloti
… to surround and stabilize their more powerful flagellar motors and provide increased contact points in the inner membrane, the peptidoglycan sacculus, and, in Gram-negative bacteria, the outer membrane. Additionally, the soil-dwelling bacterium Sinorhizobium meliloti exhibits marked …
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Identification of the Polar Flagellum Genes in Azospirillum brasilense
… flagellum but there maybe deficiencies in the flagellar motor. In our assessment of the polar flagellum genes in <em>A. brasilense</em> using functional complementation of Mot<sup>-</sup> mutants, homologs of the following genes were found: <em>fliN, flbD, flhA, flhF, fliJ, fliI, flgE, flgD, …
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Molecular modeling and simulation of bacterial chemosensory arrays
… activation and the generation of clockwise (CW) flagellar motor rotation. Specifically, I present a series of MD simulations that investigate the effect of a hyperactivating mutation at a key acetylation site and offer a molecular explanation of acetylation-dependent generation of CW flagellar …
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Use of Bioinformatics in the Analysis of Chemotaxis Proteins
… regulator CheY. Phospho-CheY binds to the flagellar motor switching the direction of rotation of the flagella and thus allowing the cell to move towards or away from the attractant or repellent. The phosphatase CheZ removes the phosphoryl group from phospho-CheY restoring default flagellar …
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Influence of pH and protein ChePep on motility of Helicobacter pylori
… to pH 7 on the epithelial surface. Both the flagellar motor and viscoelastic properties of medium depend on the pH, thus the change of motility of H. pylori across a pH gradient is complicated. The data from experiments performed by Dr. C. Su of the Bansil lab were analyzed and torque was …
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Signal Processing in Bacterial Chemotaxis
… call strain (BT3077) containing the cheY, motor and switch genes, ATP depletion, accomplished by treating the cells with arsenate, suppressed the CheY protein-induced tumbling. ATP was not required by the switch for directing the clockwise and counterclockwise rotations of the flagellar …
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Novel Perspectives on the Utilization of Chemotactic Salmonella Typhimurium VNP20009 as an Anticancer Agent
… encodes the chemotaxis response regulator of flagellar motor function, rendering the strain deficient in chemotaxis. Replacement of cheY with the 14028 wild-type copy resulted in a 70% restoration of phenotype in traditional chemotaxis capillary assays compared to the parental strain. We …
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Quantitative investigation of bacterial chemotaxis at the single-cell level
… This chain of events influences the behavior of motor proteins that drive the rotation of helical filaments called flagella. Individual cells of the gut-dwelling bacteria Escherichia coli (E. coli) have many such flagella, whose collective action results in the swimming behavior of the cell. A …
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Studies on the role of CheS in Sinorhizobium meliloti chemotaxis
… its cognate response regulator which modulates flagellar rotation. Signal termination by dephosphorylation of the response regulator is necessary for the organism to react rapidly to changes in the environment. The phosphorylated response regulator CheY in <i>Escherichia coli</i> is …
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Geometry and Hydrodynamics of Swimming with a Bundle of Bacterial Flagella
… of life starting from unicellular organisms. Flagellar propulsion is the most common form of locomotion amongst unicellular organisms. On the one hand, eukaryotic organisms have flexible flagella which they undulate in a remarkable variety of patterns - from the whip-like motion of a …
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Similarities and variations of the enterobacterial chemotaxis paradigm in Sinorhizobium meliloti
… sativa commonly known as alfalfa. It uses flagellar rotation and chemotaxis to seek roots of host plants to inhabit. This symbiosis serves as a great model system for studying biological nitrogen fixation and plant-microbe interactions. Since alfalfa brings enormous economic value to the …