Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 43 for “"fission yeast"”.
-
Cell Size Control in Fission Yeast
… clear mechanisms have yet to be elucidated. The fission yeast Schizosaccharomyces pombe is an ideal model organism whose simple but conserved cell biology has led to the identification of many important cell size regulators common to all eukaryotes. In this thesis, I have quantitatively analyzed …
-
Roles of H2A.z in Fission Yeast Chromatin
… one of the seven JmjC Domain Proteins (JDPs) in Fission Yeast. JDPs are known to function in chromatin and some act as histone demethylases. We found that Msc1 is a member of Swr1 Complex which is known to exchange histone H2A variant H2A.z in nucleosomes. We purified H2A.z as a member of Swr1 …
-
Tracking translation factors in fission yeast nucleus
Translation factors are essential components of the ribosome, yet it has been reported that many ribosomal proteins (RPs) and other translation factors are found at transcription sites of Drosophila melanogaster (D. melanogaster) polytene chromosomes. Whilst these findings might indicate the …
-
Mechanisms of genomic stability maintenance in fission yeast
DNA replication consists of two distinct steps: the licensing step during the G1 phase and the initiation step at the G1 to S transition point. During licensing, two inactive complexes of the replicative helicases, MCMs, are loaded onto all the potential origins of replication (ORIs), through the …
-
Genomic analysis of cohesin dynamics in fission yeast
… define the overall distribution of cohesin along fission yeast chromosomes. Like in budding yeast, cohesin is mainly detected at sites of convergent transcriptional termination, in the following termed convergent sites. However, only approximately half of these are bound whereas in budding yeast …
-
RNA EXPORT FACTOR ELF1 MAINTAINS GENOME STABILITY IN FISSION YEAST
Genome instability, or frequent mutations within a genome, is deleterious and a hallmark of carcinogenesis. Here, I identified that Elf1, a AAA+ family ATPase involved in mRNA nuclear export, prevents genome instability. Upon deletion, elf1Δ cells grow slowly, forming small colonies. Curiously, …
-
Control of M-G1 phase-specific expression in fission yeast
… for all eukaryotic cells. The budding and fission yeasts, Saccharomyces cerevisiae and Schizosaccharomyces pombe, have been used extensively as model organisms for the study of the eukaryotic cell cycle and cell cycle-regulated transcription, because the cell cycle machinery is conserved …
-
Pyp3 Involvement in Mitotic Control and Cell Growth in Fission Yeast
In fission yeast, Schizosaccharomyces pombe, tyrosine dephosphorylation of p34cdc2 is required for mitotic initiation and this reversible tyrosine phosphorylation plays a crucial role in the regulation of the cell cycle. The kinases wee1+, mik1+ and the phosphatases cdc25+, pyp3+ play opposite …
-
Expression of single chain antibody-encoding genes in fission yeast and tobacco
The goal of this project was to explore the expression of genes encoding single chain antibodies (SCA) in eukaryotic cells. During the course of this research, a new PCR-based strategy was developed that significantly reduced the time required for SCA gene synthesis. Although initially developed …
-
Identification and characterization of BEN1, a novel microtubule associated protein in fission yeast
In the fission yeast S. pombe, Mto1p and Mto2p are involved in MT nucleation from cytoplasmic MTOCs and recruit components of the γ-TuC. We wished to discover whether Ben1p plays a similar role. ben1<sup>+</sup> was tagged at its normal chromosomal location and the intracellular localization was …
-
Fission Yeast Exon Junction Complex Orthologs Ensure the Maturation of Meiotic Transcripts During Meiosis
… translation initiation factor eIF4aIII in the fission yeast Schizosaccharomyces pombe. Together with Mnh1, Rnps1 and Y14, Rsf1 forms the core of the Exon Junction Complex (EJC), which is essential for transcriptional surveillance and localization of mature mRNA. Using a combination of genetic, …
-
Forces Are Able to Set Position And Timing Of Microtubule Catastrophes In Fission Yeast
In the fission yeast Schizosaccharomyces pombe, interphase microtubules switch to depolymerization almost exclusively at the poles of the cylindrical cell. Proper localization of these events is crucial for morphogenesis, and is thought to depend on factors preferentially localized at the cell …
-
Biochemical and genetic analysis of a fission yeast ribonucleoprotein homologous to signal recognition particle
… A homolog of mammalian SRP RNA was found in the fission yeast, Schizosaccharomyces pombe. The gene encoding the S. pombe RNA was cloned by screening a genomic library. Sequence analysis shows that the two RNAs are structurally similar. Disruption of the chromosomal copy of the single copy 7SL …
-
Evaluating the role of the fission yeast cyclin B Cdc13 in cell size homeostasis
… change in concentration with cell size. In fission yeast, Cdc13 (cyclin B), an important activator of the core cell cycle kinase Cdc2 (CDK1), increases in concentration as cells grow. It has been proposed that the concentration of such cell cycle regulators serves as a proxy for cell size …
-
Role of Molecular Motors and Maps in Spindle Dynamics and Chromosome Segregation in the Fission Yeast Schizosaccharomyces Pombe
… mechanics. Here I used the relatively simple fission yeast to address how defects in spindle mechanics affect chromosome segregation. The metaphase spindle is maintained at a constant length by an antagonistic force-balance model yet how the regulation of metaphase spindle length contribute to …
-
Using S. pombe to Study the Biological Roles of the Histone Deacetylases Sir2 and Clr3 and the DEAD-Box RNA Helicase Ded1
The fission yeast Schizosaccharomyces pombe provides a good model system to quickly study basic mechanisms underlying biological pathways conserved in higher eukaryotes. Here we utilized fission yeast to study the roles of the histone deacetylases (HDACs) Sir2 and Clr3 in heterochromatin formation …
-
Determining Molecular Mechanisms of Cell Division in Fission Yeast by Testing Major Assumptions of the Search, Capture, Pull, and Release Model of Contractile-Ring Assembly
… in formation of two daughter cells. The fission yeast <i>Schizosaccharomyces pombe</i> is an ideal model organism to study eukaryotic cytokinesis, because the components, mechanisms, and regulatory pathways are well conserved. Cells from yeast to humans assemble the contractile ring of …
-
Characterization of nonsense mediated mRNA decay in Schizosaccharomyces pombe
… in NMD and instead they have proposed that, from yeast to mammalian cells, NMD is mostly determined by the distance of the PTC from the 3’ end. In this study, to investigate the link between pre-mRNA splicing and NMD, I used the fission yeast Schizosaccharomyces pombe. Many genes carry introns in …
-
Biochemical and genetic characterization of U1 and U3snRNAs from Schizosaccharomyces pombe
I have examined the small RNA profile of the fission yeast Schizosaccharomyces pombe. In vivo labeling followed by two dimensional gel electrophoresis of S. pombe RNA demonstrates the presence of four prominent species in the size range of interest (100-300 nucleotides). Further analysis of these …
Page 1 of 3