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Showing 1 to 20 of 22 for “"filamin"”.
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The cross-linking mechanism of filamin A in the actin cytoskeleton
… confer to the cell its mechanical properties. Filamin is an abundant actin-binding protein that efficiently cross-links actin filaments in large-angle orientations, requiring the lowest concentration to convert actin filaments into a cohesive gel. Filamin dimers are composed of two 24-repeat …
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Modeling the interaction between actin and filamin 1 in the cell cytoskeleton
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 1999.
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The Role of Rac and Filamin A in Osteoclast Function and Osteoclast Heterogeneity
… in osteoclasts lacking actin binding protein Filamin A (FLNA), which interacts with Rho GTPases to regulate osteoclast formation (osteoclastogenesis (OCG)) and function. It was observed using this technique that FLNA-knock-out (KO) osteoclasts were smaller and produced shallower Rpits compared …
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Identifizierung und Charakterisierung neuer intrazellulärer Bindungspartner von CEACAM1-L und deren Einfluss auf CEACAM1-L-vermittelte zelluläre Funktionen
In dieser Arbeit wurde Filamin A als neuer intrazellulärer Interaktionspartner von CEACAM1-L identifiziert. Um die funktionelle Auswirkung der Interaktion von CEACAM1-L und Filamin A zu untersuchen, wurde ein humanes Melanom- Zellsystem etabliert, dass die Filamin A- und CEACAM1-L-negativen …
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Diverse functions for intern associated proteins in Drosophila adult muscle
… by the enrichment of the actin-binding protein filamin/Cheerio in the palm and wished to know if it is required for palm function. Deletion of the C-terminus of filamin/Cheerio resulted in a reduction in palm length. Filamin/Cheerio is a mechanosensitive protein which exists in a closed and open …
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Identifizierung neuer Interaktionsproteine des humanen Septins SEPT4 (hCDCrel-2) in fetalem Gehirn
… charakterisiert: SEPT8, SEPT11 und die Proteine Filamin B, MURR1 und die Ubiquitin-spezifische Protease 31. Es wurde in dieser Arbeit gezeigt, dass auch der N-Terminus von SEPT4 in Interaktionen mit Septinen und anderen Proteinen involviert ist. Die Expression der in dem Yeast Two-Hybrid …
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Actin remodeling in motile cells
… state and after orthogonal cross-linking with filamins using a novel, non-perturbing fluorescence system. Human filamin A or Dictyosteliun discoidium filamin slow actin filament turnover by [approximately] 50% and recruit much of a significant population of actin oligomers that we measure are …
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Mapping the actin and actin binding proteins interactions : from micromechanics to single molecule force spectroscopy
… assay by testing it with two different ABPs: filamin, a crosslinker, and a-actinin, a bundler. We measured rupture forces of 28-73 pN and 30-56 pN for filamin/actin and a-actinin/actin respectively, suggesting that the former is a slightly stronger interaction. Moreover, since no ABP unfolding …
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The Biological Basis for Toughness in Beef
… to the extensive degradation of titin, nebulin, filamin and desmin by endogenous proteases. Rapid μ-calpain autolysis in high pHu meat was concurrent with the faster degradation of titin, nebulin and filamin in this pHu group. Conversely, faster degradation of desmin was concomitant with …
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Biomimetic cytoskeleton assemblies and living cells on micropillar force sensor arrays
… the pillar heads, with the actin binding protein filamin. Crosslinking experiments are also conducted with divalent cations and with fluorescently labelled myosin II motors. The mechanical properties of single filaments are determined by Fourier analysis of their fluctuations. Transport properties …
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Genomic Approaches to Congenital Genitourinary Disorders
… PBS brothers identified a missense mutation in Filamin A and functional testing suggests this mutation impairs muscle cell contraction and mechanosensing. Candidate genes identified from sequencing the exomes of unrelated patients are hypothesized to have similar effects on cellular function, …
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Mechanical properties of F-actin network
… networks cross-linked with various ABPs such as filamin and a -actinin, with and without gelsolin to control filament length. Microstructures of those networks were also characterized in terms of filament length, mesh size, and degree of bundling.
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Microstructurally-based constitutive models of cytoskeletal networks for simulation of the biomechanical response of biological cells
… two different types of actin binding proteins (filamin and streptavidin). The enhanced model is also used to evaluate the influence of the cross-links' torsional stiffness on the entropic bending configuration space of the cytoskeletal filaments. The 3D constitutive network model provides a …
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Identifying Common Therapeutic Targets in Merlin-deficient Brain Tumours
… Heat Shock 70kDa Protein 1A (HSPA1A) and Filamin B (FLNB) as potential common, novel therapeutic targets. We validated these candidates in human primary meningioma and schwannoma tumour cells using a variety of techniques. We also showed that specific 7 knockdown of STAT1 and PDLIM2 was …
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Domänenanalyse des Cyclase-assoziierten Proteins (CAP) und Charakterisierung von Filactin, einem neuartigen actinähnlichen Protein aus Dictyostelium discoideum
… The unique 105 kDa protein contains two filamin homologous regions in its N-terminal, and a clearly actin-related domain within the C-terminal part. While in resting cells the endogenous filactin shows cytoplasmic distribution and interaction with protein aggregates as well, the GFP …
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MOLECULAR MECHANISMS UNDERLYING CLINICAL BEHAVIOR OF NEUROENDOCRINE TUMORS: STUDY OF NEW POSSIBLE BIOMARKERS PREDICTABLE OF PHARMACOLOGICAL RESISTANCE AND TARGETS
… Moreover, the cytoskeleton actin binding protein filamin-A (FLNA) emerged as an important player in the regulation of PitNETs drug responsiveness and invasiveness. At this regard our data showed that activation of the cAMP pathway and DRD2 agonist regulated FLNA activity by increasing or …
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Investigating DNA Methylation Changes in a Preclinical Murine Model of Cutaneous Squamous Cell Carcinoma
… methylation in the tumor suppressor gene Filamin A interacting protein 1 like (Filip1l). Down-regulation of the FILIP1L gene has been linked to ovarian, prostate and pancreatic cancer aggressiveness and metastatic potential and is an independent prognostic marker in ovarian cancer …
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THERAPEUTIC POTENTIAL OF TARGETING THE FLNA-REGULATED WEE1 KINASE PATHWAY IN ADRENOCORTICAL CARCINOMAS.
… benign adenomas (ACA), the cytoskeletal protein filamin A (FLNA) is poorly expressed in ACC. Of note, its presence, even at low levels, is associated to a less aggressive tumor behaviour, potentially due to its role in negatively regulating the insulin-like growth factor 1 receptor (IGF1R) …
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