Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"fibrogenesis"”.
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The desmoplastic response : mechanisms of tumour-induced fibrogenesis
The main concern of this thesis is with desmoplasia - a process in which excessive connective tissue is deposited in a neoplasm. This is a common phenomenon in neoplasia but one whose mechanisms are poorly understood. To study the process, I used a human malignant melanoma cell line (UCT-Mel 7) …
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TGF-beta,Smad signaling in hepatic stellate cells and during liver fibrogenesis
… elucidate TGF-beta target genes responsible for fibrogenesis, an analysis of Smad7-dependent mRNA expression profiles in HSC was performed, resulting in identification of the inhibitor of differentiation 1 (Id1) gene. Ectopic Smad7 expression reduced Id1 expression. Conversely, Id1 overexpression …
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Morphological characterization of liver fibrogenesis in animal models with genetically modulated TGF-beta signal transduction
… is impaired. In a healthy liver, synthesis (fibrogenesis) and degradation (fibrolysis) of matrix tissue are in balance. Fibrosis occurs when scar tissue is generated in excess and faster than it is degraded and removed from the liver. TGF-beta is known to play a central role in regulation of …
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Adipogenesis and fibrogenesis in muscle and effects of pregnancy and feeding level on carcass meat quality and development of cattle fetus
O presente trabalho foi desenvolvido a partir de três experimentos.O Objetivo do primeiro estudo foi de avaliar a deposição de tecido adiposo intramuscular e colágeno em músculo esquelético de animais da raça Wagyu em comparação com animais da raça Angus. Os animais foram manejados sob mesmas …
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Unfolded Protein Response (UPR) Signaling in Hepatic Stellate Cells (HSC) as a Key Regulator of Liver Fibrosis
… R-like ER Kinase (PERK) as a crucial factor for fibrogenesis, but the mechanisms of PERK signaling in HSCs are largely unknown. Our research aims to understand how PERK regulates HSC activation and fibrogenesis and to target these mechanisms in order to promote HSC inactivation and mitigate …
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Microfabricated Fibrotic Microtissue Array for Screening of Potential Anti-Fibrotic Treatments
… changes in tissue mechanics during fibrogenesis. In this study, we created novel micropillar microdevices, which allow thin and membranous fibroblast microtissues to be formed, similar to the morphology of lung alveolar tissue. We show the capability of these devices to replicate the …
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TGF-β Signaltransduktion in der experimentell induzierten Leberfibrose durch die Gallengangsligatur bei der Ratte
… in vivo data regarding Smad activation during fibrogenesis are scarce. Methods: Here, liver fibrosis was assessed subsequent to bile duct ligation by determining liver enzymes in serum and collagen deposition in liver tissue. Activated hepatic stellate cells were identified by …
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The role of caspase-1 in liver and adipose tissue during metabolic dysregulation in mouse models on NASH
… a protection from MCD-induced inflammation and fibrogenesis, suggesting that caspase-1 in Kupffer cells plays an important role in NASH pathogenesis. Casp1-/- mice on the high fat diet gained less weight than wild type mice, but had greater adiposity. Casp1-/- mice on the high fat diet were …
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The epithelial-mesenchymal transition of hepatocyte in liver cirrhosis
… to both hepatocyte dysfunction and liver fibrogenesis. To address this issue, hepatocytes were isolated from normal rat liver and from dimethylnitrosamine (DMN)-induced fibrotic rat livers. Incubation of primary rat hepatocytes with TGF-β1, the central cytokine in fibrogenesis, and …
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Discovery of Peptide and Aptamer Ligands for Targeted Drug Delivery to Hepatic Stellate Cells
… and therapy of liver fibrosis. The mechanism of fibrogenesis and potential treatments of liver fibrosis are summarized in Chapters 1 and 2. In Chapter 3, we identified HSC-specific peptides using a peptide phage display for diagnosis and treatment of liver fibrosis. Caused by chronic injuries, …
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Kryokonservierung von hepatischen Sternzellen der Ratte
… HSC of the rat are used as a model of liver fibrogenesis. Many proceedings are studied on this cells, for example the biotransformation, the gene expression, the effect of pharmaceuticals, different kinds of transcriptional factors and many more. Methods for the isolation of rat hepatic …
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Cell cycle regulation in the liver : differential functions of E-type cyclins E1 and E2 for G1/S-phase transition and endoreplication in mice
… contribution of E-type cyclins for liver fibrogenesis. In this context, over-expression of the proto-oncogene c-myc in murine hepatocytes was shown to serve as an interesting new model for liver fibrosis which is dependent on cyclin E1 and E2. Accordingly, alb-myctg mice are prone to …
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Die Bedeutung von Fetuin-A für Proliferation und Fibrogenese im Knockout-Modell
… for physiological proliferation and pathological fibrogenesis of the liver. The rational was, that fetuin-A as a TGF-beta antagonist possibly adjusts the effects on proliferation and fibrogenesis of this growth factor and therefore the loss of the fetuin-A availability in the knockout model leads …
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Silencing of endoglin in rat hepatic stellate cells by RNA interference identifies endoglin as an important component of the transdifferentiation process
… endoglin is involved in the process of liver fibrogenesis. To investigate these questions, RNAi was used for the first time in this laboratory in order to silence endoglin. By transfecting HSC with siRNA oligonucleotides endoglin was down-regulated very efficiently. Subsequently, endoglin …
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Therapeutic application of dominant-negative PDGF beta-receptor in liver fibrosis
… latent TGF-Beta activator, leading to decreased fibrogenesis as proven by reduced levels of collagen type I(Alphal) mRNA compared to the control cultures.We then used a bile duct ligation (BDL) rat model to examine the effects of sPDGFRBeta on hepatic fibrogenesis. It is known that expression of …
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Untersuchungen zur TGF-[beta]1-Expression und -Bioverfügbarkeit bei der Leberfibrogenese
… is one of the most important cytokines mediating fibrogenesis in the liver and in other organs. The major portion of it is secreted as latent molecule complexed either only with the “latency associated peptide” (LAP) or with LAP and the “latent TGF-Beta binding protein” (LTBP). Activated hepatic …
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Production of prostaglandin E2 and thromboxane A2 by rat liver macrophages and involvement of nitric oxide and cytokines in mediator pathways under inflammatory conditions
… play opposing roles in the development of liver fibrogenesis: IL-1β, TNFα, IL-6, transforming growth factor (TGF)-β and TxA2 (pro-fibrogenic mediators) promote whereas PGE2, IL-10 and nitric oxide (anti-fibrogenic mediators) suppress liver fibrogenesis. The present study shows the production of …
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Heterozygote Hämochromatose-Genmutationen als genetische Progressionsfaktoren bei chronischer Hepatitis C
… of HFE mutations as primary risk factors for fibrogenesis and disease progression in chronic hepatitis C.
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The expression profile of cytoglobin in human fibrotic lung, and the protective role of cytoglobin in hypoxia and oxidative stress in vitro
… in response to hypoxia, oxidative stress and fibrogenesis. Presented here is evidence of CYGB expression within cells of fibrotic lesions taken from patients with Idiopathic Pulmonary Fibrosis (IPF) and Chronic Obstructive Pulmonary Disease (COPD). CYGB staining was observed in fibroblasts, …
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Development of Protein Based PCBP2 siRNA Nanocomplex for Liver Fibrosis Therapy
… background about RNA interference (RNAi), liver fibrogenesis, markers of liver fibrosis, and the role of PCBP2 in liver fibrogenesis. We also presented the Statement of the Problems and Objectives. In chapter 2, we reviewed the avidin-biotin technology and its potential applications in …
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