Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 97 for “"fibroblast cells"”.
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The Cytotoxic Effects of Malondialdehyde on Human Lung Fibroblast Cells
… the toxic effects of MDA on the human lung fibroblasts MRC5, which have an intact p53 response, and their SV40 transformed counterpart, MRC5 SV2, which have a sequestered p53 response. Both cell lines were treated with MDA (0-1000 µM) for 24 and 48 h and subjected to a variety of analyses to …
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Characterization of PCSK9-mediated LDLR Degradation in Hepatic and Fibroblast Cells
… on LDLR levels were detected in several lines of fibroblast cells despite normal LDLR-dependent cellular uptake of PCSK9. Importantly, a PCSK9 degradation assay showed that 125I-labeled wild-type PCSK9 was internalized and degraded equally in both hepatic and fibroblast cells, indicating …
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CHARACTERIZATION OF HCMV-ENCODED CHEMOKINE RECEPTOR US28 TRANSDUCED MSU1.1 HUMAN FIBROBLAST CELLS
… if US28 is oncomodulatory in normal human cells, we engineered the human fibroblast cell line MSU1.1 to express US28 via lentivirus infection. MSU1.1 cells were transduced with pHAGE-US28-ZsGreen. Fluorescent confocal microscopy was utilized to detect the ZsGreen tag and confirmed the …
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Exposure to fluorescent excitation light induces dose-dependent, irreversible force relaxation in living fibroblast cells
The student, Samantha Knoll, submitted this Dissertation for approval on 2016-04-18 at 15:13.
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The M(1) muscarinic receptor and its second messenger coupling in human neuroblastoma cells and transfected murine fibroblast cells
… cyclase system in human neuroblastoma SH-SY5Y cells. The maximal carbachol(CCh)-stimulated [³H]IP₁ accumulation in the SH-SY5Y cells was decreased in the presence of 1 μg/ml pertussis toxin, suggesting that a pertussis toxin sensitive G-protein may be involved in the coupling. Several cell …
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The Use of Ultrasound-Targeted Microbubble Destruction in Mediated Delivery of a Gadolinium Magnetic Resonance Imaging Contrast Agent Into Fibroblast Cells In Vitro
… ultrasound-targeted microbubble destruction into fibroblast cells in vitro is presented. First, MRI contrast agents Gd-F-DOTP-ME and Gd-DOTA-4iBA are shown to associate with liposome microbubbles used as ultrasound contrast agents. Repeated washings with phosphate buffered saline followed by T1 …
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Evaluating the effects of acoustic stimulation on fibroblast cell migration and muscle fibre formation.
Cells are known to interact and respond to external mechanical cues and recent work has shown that application of mechanical stimulation, delivered via acoustic vibration, can be used to control complex cell behaviours. Much of the work in this area has focused on the effects of mechanical …
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Targeting neurodegenerative phenotypes and mitochondrial dysfunction in a Drosophila Melanogaster model of Parkinson’s Disease
… factor, using Drosophila melanogaster and human fibroblast cells as models for PD. The multidisciplinary approach investigated the mutations in Drosophila genotypes, including W1118, PINK1B9, NUBPL, and the double mutant PINK1B9, NUBPL. Mitochondrial function was evaluated using Drosophila's …
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The Expression of T-Box (Tbx3) in the Bovine Mammary Gland
… and Tbx3 expression in bovine mammary epithelial cells (MEC), the cell responsible for milk production. To test our hypothesis, MAC-T cells (MEC line) were treated with GH at 100 (GH100) or 500 (GH500) ng/mL or IGF-I at 100 (IGF100) or 200 (IGF200) ng/mL for 24 and 48 hours. As determined by …
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Development Of Strontium-Alginate Scaffolds To Improve Gingival Fibroblast Function
… material in cell culture model of human gingival fibroblast cells. Materials and Methods: Specially designed molds were fabricated and used for the development of the strontium alginate scaffolds with different strontium concentrations to be administered both directly and indirectly to the human …
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Correction of Cystic Fibrosis-Specific Induced Pluripotent Stem Cells
… modify the resulting induced pluripotent stem cells (iPSCs) have provided an alternative to conventional gene therapies thus far attempted for CF. Here we describe the <em>in vitro</em> generation of iPSCs starting from fibroblast cells obtained from a CF patient. These cells, like embryonic …
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Engineering Fluorescently Labeled Human Fibroblast Growth Factor One Mutants and Characterizing Their Photophysics Properties Towards Designing FRET Assays
<p>Human fibroblast growth factor one (hFGF1) belongs to a family of 22 FGF members produced by fibroblast cells. Cell signaling during physiological processes of angiogenesis and wound healing occurs when hFGF1 binds to its receptor (FGFR). However, when heterogenous homeostasis is not maintained, …
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Exploring the influence of adipokines on neuronal function in Alzheimer’s disease
… death. In this study, human neuroblastoma SHSY5Y cells were differentiated using retinoic acid to demonstrate neuronal-like characteristics which was confirmed by immunocytochemistry (ICC). The pre-adipocytes 3T3-L1 cells, were chemically differentiated to exhibit adipocyte-like characteristics …
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Coating of Polyvinylchloride for Reduced Cell / Bacterial Adhesion and Antibacterial Properties
… with antibacterial properties. 3T3 mouse fibroblast cells were used for cell adhesion, Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus were used for bacterial adhesion in the first study, Pseudomonas aeruginosa and Staphylococcus aureus were used for bacterial adhesion …
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Evaluation of the antimicrobial properties and biocompatibility of polypropylene mesh conjugated with gold nanoparticles
… in vitro cell culture assays using L929 murine fibroblast cells.
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Isolation And Characterization Of Natural Antimicrobials From Plant And Marine Organisms
… proliferation was decreased when mouse embryonic fibroblast cells, NIH 3T3 were treated with the compounds, proving they are cytotoxic to varying degrees.</p>
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Micronuclei induction in AG01522 cells is independent of temperature and linear energy transfer
… biological effects in nonirradiated cells that have received signals from irradiated cells. In a co-culture experiment, the bystander signaling is proposed to occur via the medium. Using a co-culture setup, the work in this thesis investigates the effects of temperature as an …
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HYPOXIA-REGULATED EXPRESSION OF GLUT-1 IN GBM CELL LINES
<p>Unlike normal cells, cancer cells can grow in low oxygen (hypoxic) environments. Changes in relative oxygen concentration can alter gene expression in tumors to allow for their selective growth. The result of such changes allows the tumor to adapt its cellular metabolism and promote tumor …
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Transcending the Cell Fate Stereotype: Cell Penetrating Peptide Technology helps Induce Pluripotency in Multiple Cell Types
<p>Induced pluripotent stem cells (iPSCs) are one of the most promising new technologies in the field of regenerative medicine. iPSCs can be generated directly from a patient’s own fibroblast cells then converted into any cell type, thus overcoming challenges associated with embryonic stem cells …
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A study investigating the mechanical testing of a novel dental restorative material and its biocompatibility
… both directly and indirectly with ATCC mouse 3T3 fibroblasts and undifferentiated pulpal fibroblast cells (OD21 cells). These determined that all three materials were cytotoxic to both the cell types, however a one-way ANOVA test showed that there was no significant difference between the …
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