Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"fibrillization"”.
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Temperature dependence of amyloid beta-protein fibrillization
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Physics, 1999.
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Effects of glycosylation on the structure and fibrillization of prion protein fragments
… (at Asn 181) significantly reduces the rate of fibrillization by promoting intermolecular disulfide formation via Cys 179. Furthermore, the aggressive fibrillization of a C179S mutant of this fragment highlights the significant role of disulfide stability in retarding the rate of fibril …
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Investigating the process of fibril formation of the Iowa mutant of the Alzheimer's peptide
… Alzheimer’s disease, has been shown to undergo a fibrillization process that involves numerous intermediate stages. One of the intermediate stages has revealed a high neurotoxicity and is believed to be the cause of neurodegeneration associated with Alzheimer’s disease. Various point mutations of …
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Pathogenesis of Neurodegenerative Diseases via Templated Recruitment
… by in vitro studies showing nucleation-dependent fibrillization of tau, we tested the hypothesis that preformed tau fibrils (tau pffs) assembled from recombinant protein may act as seeds to nucleate the fibrillization of soluble tau in cultured cells. Indeed, we found that minute quantities of tau …
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Growth and Toxicity of Amyloid β Polymorphs
… of understanding of the intricate process of Aβ fibrillization. We investigated the unknown mechanism of action of a potential Alzheimer’s drug candidate, bexarotene, on Aβ fibrillization using powerful techniques. Bulk kinetics studies suggested that bexarotene interferes with the primary …
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Domain swapping as a molecular mechanism in amyloidosis
… already in the beginning of the lag phase of the fibrillization reaction. The appearance of these doughnut-shaped oligomers is similar to that described for oligomers of other amyloidogenic proteins. Purified oligomers of cystatin C were shown to fibrillize faster and at a lower concentration than …
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Microglial activation and regulation by secreted chaperones
… manner. Extracellular LRPAP-1 inhibited Ab fibrillization. In mixed neuronal-glial cultures, extracellular LRPAP-1 increased Ab bound and/or internalised by neurons but reduced Ab neurotoxicity. Thus. LRPAP-1 can be released by stressed microglia to inhibit microglial phagocytosis, inhibit …
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Dynamic Measurements with Scanning Probe Microscopy: Surface Studies Using Nanostructured Test Platforms of Metalloporphyrins, Nanoparticles and Amyloid Fibrils
… probe microscopy. The progressive growth and fibrillization of amyloid â over extended time intervals was visualized with high resolution using AFM.
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Structural Elucidation of the Interaction Between Neurodegenerative Disease-Related Tau Protein with Model Lipid Membranes
… However, two questions remain: What is tau's fibrillization mechanism, and what is tau's cytotoxicity mechanism? Tau is prone to heterogeneous interactions, including with lipid membranes. Lipids have been found in NFTs, anionic lipid vesicles induced aggregation of the microtubule binding …
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Development and Application of Solid-State NMR Methods for Investigating Protein Structure and Dynamics
… at S2, Y13, A19, or T29 to arginine inhibit fibrillization at pharmaceutical concentrations of glucagon and are promising analogues that would have longer shelf-life in solution compared to wild-type glucagon. The influenza matrix-2 protein (M2) conducts protons across the lipid membrane in …
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Unzipping Amyloid Fibrils: How a Novel Calcium-Binding Protein, NUCB1, Prevents the Formation of Amyloid Fibrils
… to sNUCB1, inhibits and disaggregates hIAPP fibrillization. Finally we have also extended this work to other amyloidogenic proteins namely tau K19(C322S), a synthetic construct comprising microtubule binding domain of human tau protein. Specifically, we show that Ca<sup>2+</sup>-free sNUCB1 …
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Formation and Pathogenicity of Cytotoxic Curli Intermediates
… microscopy in the mature curli indicating high fibrillization and compact structure of these aggregates, not seen in the intermediates. Due to known interactions of curli with eDNA in the biofilm, next we investigated the DNA content of the complexes. We hypothesized that the mature structured …
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Hydrogen-deuterium exchange studies by mass spectrometry: A method for obtaining conformational structures of proteins in solution
… ESI MS. CR is known as an inhibitor of insulin fibrillization by binding at the C-terminus of the B-Chain of insulin. Remarkably, the ESI mass spectrum of labeled protein in the presence of CR shows bimodal pattern, suggesting that CR binds with the partial unfolded intermediate structure of the …
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Development of multifunctional compounds for the modulation and detection of amyloid beta species
… we have reported that Cu2+ ions can slow down Aβ fibrillization and stabilize Aβ oligomers, and thus small molecules that can inhibit the interaction between metal ions and Aβ peptides, can be used as potential therapeutic compounds for AD. Positron emission tomography (PET) is a functional …