Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 42 for “"fetal liver"”.
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Experimental models with specific approaches to augment human fetal liver cell engraftment
… models with specific approaches to augment human fetal liver cell engraftment Pradeep Patil Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy at University of Gothenburg Background: Liver disease is a common cause of morbidity and mortality worldwide. Orthotopic liver …
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Finding lncRNAs in bone marrow and fetal liver erythroid progenitor cells in mice
… of all mammals and occurs primarily in the liver during embryogenesis and then in the bone marrow during adulthood. In spite of the different microenvironments of the liver and bone marrow, current research shows that the majority of protein-coding genes important to crythropoiesis are …
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The effect of a zinc deficiency and alcohol intake during gestation in the rat
… maternal status by decreasing weight gain, liver Zn and plasma Zn concentration. Litter size, litter weight, and fetal liver and brain weight were decreased only in the alcohol zinc deficient group compared to adequately fed controls. The concentration and total quantity of fetal liver Zn …
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Blood Reservoir Function of the Fetal Guinea Pig Liver
<p>To determine of the fetal liver functions as a blood reservoir, we measured liver blood volume under normal conditions and after hemorrhage and volume loading. Nineteen pregnant guinea pig sows and 54 near-term fetuses were injected intravenously with <sup>51</sup>Cr-labeled red cells while the …
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A THREE DIMENSIONAL MODEL OF HUMAN HEPATO-BILIARY ORGANOGENESIS
… have emerged aimed at generating functional liver tissue by using decellularized liver scaffolds repopulated with liver cells. The quest continues for identifying the best cell source for liver tissue engineering, but one cell source in particular, human fetal liver progenitor cells (hFLPCs), …
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Development of a Human Immune System from Hematopoietic Stem Cells in a Human/Mouse Xenogeneic Model
… While the implantation of SCID mice with human fetal thymus and liver (SCID-hu thy/liv mice) allows for the development of abundant thymocytes that are localized to the human organoid implant, there is minimal systemic repopulation with human T cells. Here I present evidence that transplantation …
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Gamma-Glutamyltransferase- Is it a Biomarker of oxidative stress?
… were used to induce oxidative stress in human fetal liver (HFL cells) and human renal proximal tubules epithelial cells (hRPTE cells). The results suggest that there is an increased excretion of GGT into the extracellular medium for hRPTE cells and an increase in GGT level for HFL cells. The …
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Application of in vitro erythropoiesis from bone marrow derived progenitors to detect and study genotoxicity
… a toxicity assay, this thesis work (a) adapted a fetal liver-based in vitro erythropoietic culture system to induce optimized erythropoietic growth from the lineage-marker-negative (Lin) population in adult BM, as adult hematopoietic tissue is ultimately a feasible source of cells; and (b) …
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Characterization of Thy-1 expression on human hematopoietic cells
… 5E10 staining of hematopoietic cells from human fetal liver, cord blood, adult peripheral blood, and bone marrow samples revealed that Thy-1 expression is restricted to approximately 1-4% of fetal liver, cord blood and bone marrow cells. These consist of a small subset of lymphoid cells and …
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Transcriptional consequences of genetic variation on single cells in development and cancer
… CNVs, I constructed three scRNA-seq atlases of fetal tissues (adrenal gland, kidney and liver) that carry constitutional whole-chromosome CNVs (i.e. aneuploidy). I investigated and demonstrated the impact of these CNVs on both the cell type composition and the transcriptome of various cell types …
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C/EBPalpha downregualtes c-jun expression
… increase of c-jun mRNA in C/EBPa knockout mice fetal liver was observed. c-jun, a component of the AP-1 transcription factor complex and a co-activator of the transcription factor PU.1, is important for monocytic differentiation. Here we report that C/EBPa downregulates c-jun expression to drive …
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Hepatic microsomal and peroxisomal docosahexaenoate biosynthesis during piglet development
… Microsomes and peroxisomes were prepared from livers of fetal and neonatal piglets by a combination of differential and gradient layer centrifugation. Microsomes, peroxisomes and combined cell fractions were incubated with [13C-U]-18:3n-3. The [M] and [M+18] isotopomers of the fatty acids in …
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Methylome and Transcriptome Profiling of Hepatocytes Derived from Human Pluripotent Stem Cells
Six hundred million people suffering from liver diseases worldwide of which the lethality is two million. Freshly isolated hepatocytes from the liver have been used for transplantation purposes and are extensively used to recapitulate drug metabolism. However, they lack stem cell ability and …
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Maternal diet and programming of embryo and fetal development in the mouse
… made in adult life, those made during fetal life tend to become permanent and irreversible (i.e. programmed). This is due to the existence of sensitive periods which are brief windows of growth and development in early life during which there is an increased susceptibility to …
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Developmental and Growth Hormone Regulation of the Expression of Liver-Enriched Transcription Factors in Bovine Liver
Liver gene expression changes during development and is affected by growth hormone (GH). These changes in gene expression may be due to the differential expression of the liver-enriched transcription factors (LETFs). To study the potential involvement of LETFs in the regulation of gene expression …
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Assessing fetal topoisomerase IIα as a mechanism of in utero benzene toxicity in generating double-stranded DNA damage in CD-1 mice
… culture derived from gestational day (GD) 14 fetal livers, exposure to BQ for 24 hours showed a concentration-dependent decrease in topo IIα activity, which was not protected by treatment with N-acetylcysteine. BQ exposure (12.5 µM) is sufficient to increase topo IIα-DNA adducts, alter the …
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Insulin-Like Growth Factor-Binding Protein 2 Supports Hematopoietic Stem Cell Expansion: From In Vitro to In Vivo
… a group of growth factors isolated from a fetal liver HSC supportive cell population, improved ex vivo expansion of HSCs. Here we demonstrate that insulin-like growth factor binding protein 2 (IGFBP2), secreted by a tumorigenic cell line, also enhanced ex vivo expansion of mouse HSCs. As …
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