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Showing 1 to 20 of 49 for “"ferredoxin"”.
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Structures of oxalate oxidoreductase : C₂ activation by a microbial TPP-dependent ferredoxin oxidoreductase
… a member of the larger superfamily of 2-oxoacid:ferredoxin oxidoreductase (OFOR) enzymes. OFORs are found in microorganisms across all domains of life, and are responsible for performing a number of essential metabolic reactions, including the conversion of pyruvate to acetyl-CoA. Though, most …
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Role of Ferredoxin in Hydrogen Metabolism of Ruminococcus Albus 7 and Selenomonas Ruminantium Hd4
Made available in DSpace on 2014-12-14T15:29:38Z (GMT). No. of bitstreams: 1 7606767.pdf: 5668685 bytes, checksum: 775da1b14f7c8f7bce76672ca4601b22 (MD5) Previous issue date: 1975
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Characterization of ferredoxin and flavodoxin as molecular indicators of iron limitation in marine eukaryotic phytoplankton
Expression and regulation of the ferredoxin and flavodoxin proteins in marine phytoplankton were investigated to assess their utility as biomarkers of iron limitation. A phylogenetic survey of seventeen microalgal species showed flavodoxin induction, with accompanying ferredoxin repression, to be a …
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Characterization of ferredoxin and flavodoxin as molecular indicators of iron limitation in marine eukaryotic phytoplankton
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1997.
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Expanding the genetic toolbox for methanogenic archaea and investigating ferredoxin redox circuit formation in Methanosarcina acetivorans
… In Chapter 2, I report the finding that a single ferredoxin is the primary electron donor for phospholipid saturation in Methanosarcina acetivorans, enhancing our understanding of ferredoxin dependent redox circuits and lipid biosynthesis in methanogens. In Chapter 3, I report the sat gene as a …
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Molecular biology and biochemical characterization of the CO dehydrogenase-linked ferredoxin from Methanosarcina thermophila strain TM-1
The CO dehydrogenase~linked ferredoxin from acetate-grown <i>Methanosarcina thermophiIa</i> was characterized to determine the structure and biochemical properties of the iron-sulfur clusters. Chemical and spectroscopic analyses indicated that the ferredoxin contained two [4Fe-4S] clusters per …
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Occurrence of pyruvate:ferredoxin oxidoreductase in Campylobacter, Wolinella, Helicobacter and Arcobacter species
Pyruvate:ferredoxin oxidoreductase activity was demonstrated in microaerophilic members of Campylobacter, Wolinella, and Helicobacter. Arcobacter cryaerophila (sic) and Arcobacter nitrofigilis, two aerobic species, lacked any detectable activity. Under anaerobic conditions crude extracts of …
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Biochemical characterization of a novel iron-sulfur flavoprotein from Methanosarcina thermophila strain TM-1
… either H2 or CO as the reductant. Addition of ferredoxin A to the reaction further stimulated the rate of Isf reduction. These results suggest that Isf homologues are coupled to ferredoxin in electron transfer chains in methanoarchaea with diverse metabolic pathways. Reconstituted systems …
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Engineering protein components for living electronics
… Herein, I describe my efforts to engineer ferredoxin and flavodoxin electron transfer proteins as components in controlling electron flow in cells. In these studies, I probe the tolerance of a cyanobacterial flavodoxin to insertion of a small octapeptide, which elongates the primary …
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Studies on the carbon monoxide dehydrogenase enzyme complex present in acetate-grown Methanosarcina thermophila strain TM-1
… of: (i) CO dehydrogenase complex, (ii) a ferredoxin, and (iii) purified membranes with associated hydrogenase and b-type cytochrome. The ferredoxin was a direct electron acceptor for the CO dehydrogenase complex. The molecular weight of the isolated protein was 16,400, and the apparent …
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Studies on cytochromes and electron transport in Methanosarcina thermophila strain TM-1
… using carbon monoxide dehydrogenase, ferredoxin, membranes, and heterodisulfide reductase. Both membranes and ferredoxin were required for reduction of the heterodisulfide.
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Metabolic and molecular responses to interspecies hydrogen transfer between Ruminococcus albus and Wolinella succinogenes
… its plasmid pRumal01. The electron-bifurcating ferredoxin- and NAD-dependent [FeFe]-hydrogenase, HydABC, couples proton reduction using nicotinamide adenine dinucleotide (NADH) to proton reduction using reduced ferredoxin (Fdred), producing molecular hydrogen: 3 H+ + NADH + Fdred → 2 H2 + NAD+ + …
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Biochemical analysis of a potential drug target in the human protozoal pathogen Trichomonas vaginalis
… target hydrogensosomal enzymes such as pyruvate: ferredoxin oxioreductase and ferredoxin, to treat trichomoniasis caused by T. vaginalis. Recent studies have shown alternative pathways responsible for activating metronidazole without the involvement of hydrogenosomal enzymes. This pathway requires …
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Engineering of an efficient and enantioselective biocatalyst for the preparation of chiral pharmaceutical intermediates
… their auxiliary electron transport proteins, ferredoxin and ferredoxin reductase in vitro. The mutants were then used to catalyze the hydroxylations of a range of different substrates using whole-cell assays to investigate the changes in product ee. In addition, an efficient biocatalytic …
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Electron Transport in Methanosarcina: Pathway Heterogeneity Within the Genus
… utilize hydrogenases to transfer electrons from ferredoxin to methanophenazine. This thesis characterizes electron transport in the marine methanogen Methanosarcina acetivorans. Genomic comparison of hydrogenase gene clusters reveals that M. acetivorans encodes three putative Ni-Fe hydrogenases …
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Nitric Oxide in the Cytotoxicity of Metronidazole Against Trichomonas Vaginalis
… and (2) the enzymatic mixture consisting of ferredoxin oxidoreductase, ferredoxin, and NADPH, NO was only detected by EPR following the addition of ascorbic acid. On the other hand, the nitro anion radical was consistently produced at concentrations of >40 muM when 50 mM metronidazole was …
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Studies on two nickel-containing enzymes from Methanosarcina thermophila TM-1
… by either titanium (III) citrate or reduced ferredoxin. Reactivation with ferredoxin was a simplification over previously reported reactivation systems. ATP stimulated, but was not required for reactivation. The CO dehydrogenase enzyme complex from M. thermophila was purified and separated …
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Electron transfer and protein engineering studies of the soluble methane monooxygenase from Methylococcus capsulatus (Bath)
… Chapter 3. Expression and Characterization of Ferredoxin and Flavin Adenine Dinucleotide-Binding Domains of the Reductase Component of Soluble Methane Monooxygenase from Methylococcus capsulatus (Bath) ... Chapter 4. Intermolecular Electron Transfer Reactions in Soluble Methane Monooxygenase …
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Biohydrogen as a Fuel: Understanding and Engineering Hydrogenase Enzymes for Biotechnological Applications
… of a fusion between E. coli Hyd-3 and a ferredoxin from Thermotoga maritima, was characterised and shown to exhibit in vivo H2 evolution when co-produced with a heterologous pyruvate: ferredoxin oxidoreductase. This allowed pyruvate to be employed as a new electron donor for H2 …
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The relationship between iron and nitrogen fixation in Trichodesmium spp.
… flavodoxin a non-Fe containing substitute for ferredoxin. I determined that high isiB gene expression corresponded to cell-specific reductions in N fixation rates in both phylogenetic clades of Trichodesmium grown on varying levels of Fe. Using these laboratory-determined thresholds, I assessed …
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