Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"fed-batch cultivation"”.
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Stoichiometric medium design and nutritional control in fed-batch cultivation of animal cells
Thesis (Sc. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1997.
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Developing scalable and modular technologies for continuous biopharmaceutical production
… Generally, commercial-scale manufacturing using fed-batch cultivation and fixed infrastructure is concentrated in developed nations and results in process cycle times of weeks or months. Coupled with the complex logistical challenges associated with continuous 'plant-to-patient' cold-chains, the …
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Probing Control of Glucose Feeding in Escherichia coli Cultivations
… coli</i>. A difficulty encountered in cultivations of <i>E. coli</i> is the accumulation of the metabolic by-product acetate which inhibits cell growth and production of a desired protein. Formation of acetate occurs under anaerobic conditions but also in situations with excess of the …
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Development of a regenerable glucose biosensor probe for bioprocess monitoring
… for 16.5 continuous hours during a 20 L fed-batch cultivation of E. coli in minimal medium. Michaelis-Menten enzyme kinetics were used as an empirical model for the calibration of the experimental biosensor. The development of a computer-controlled prototype glucose biosensor and …
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Improving the recovery of "difficult to release" periplasmically-expressed products from recombinant E. Coli
… of anti-lysozyme Fab D1.3 were determined during fed-batch cultivation of E. coli. It was shown that the Fab produced as both soluble and insoluble forms and released into the culture medium over the course of fed-batch fermentation. Purification of Fab D1.3 was then performed using various …
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The process intensification of biological hydrogen production by Escherichia coli HD701
… then by changing the medium (two differing fed batch media were chosen; each medium used was experimentally linked with multiple feeds) and finally by utilising the novel technique of combining gas blending with fed batch cultivation to ensure microaerobiosis during growth. This, along with …