Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"fecal DNA"”.
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DEVELOPMENT OF FECAL DNA SAMPLING METHODS TO ASSESS GENETIC POPULATION STRUCTURE OF GREATER YELLOWSTONE BISON
… bison herds. I evaluated the feasibility of fecal DNA sampling for genetic analyses of wild bison populations. I used matched blood and fecal samples from eight radio-collared bison from Hayden Valley breeding group (YNP), and multiplex polymerase chain reaction (PCR) of four microsatellite …
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DEVELOPMENT OF FECAL DNA SAMPLING METHODS TO ASSESS GENETIC POPULATION STRUCTURE OF GREATER YELLOWSTONE BISON
… bison herds. I evaluated the feasibility of fecal DNA sampling for genetic analyses of wild bison populations. I used matched blood and fecal samples from eight radio-collared bison from Hayden Valley breeding group (YNP), and multiplex polymerase chain reaction (PCR) of four microsatellite …
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Noninvasive tracking of jaguars (Panthera onca) and co-occurring Neotropical felids in Belize, Central America by genotyping feces and remote camera trapping
… for species and individual identification using fecal DNA as he primary DNA source. All microsatellite loci examined successfully cross-amplified in the three target species, and were polymorphic. Second, to maximize PCR amplification success and genotyping accuracy rates, and to minimize …
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A Comparison of Wildlife Monitoring Techniques in Riparian Ecosystems of the Western United States
… camera trapping, GPS devices, mark-recapture, fecal DNA surveying, circular plot point counts, mist-net transects and bird-banding, transect counts, and nest monitoring. The main objectives of wildlife monitoring include gaining data in species presence, absence and distribution, relative …
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A Novel SNP Genotyping Technique to Determine Orangutan Relatedness and Genetic Diversity at Camp Leakey in Tanjung Puting, Central Kalimantan
… methyl based magnetic capture for enrichment of fecal DNA and commercial human targeted single nucleotide polymorphism (SNP) microarray technology. I confirmed results of this new genotyping technique through standard microsatellite short tandem repeat (STR) micro-capillary genotyping. I estimate …
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The Influence of Diet on the Mammalian Gut Microbiome
… perform a comparative metagenomic study of the fecal communities of 39 diverse mammals to assess microbiome variation. These animals included herbivores, omnivores and carnivores representing different portions of the mammalian tree, Targeted sequencing of the bacterial 16S rRNA gene and shotgun …
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Spatial ecology and demography of eastern coyotes (Canis latrans) in western Virginia
… five scat surveys across 2.5 years and extracted fecal DNA to identify individual coyotes in a mark-recapture framework. I estimated coyote densities in Bath (5.53 – 9.04 coyotes/100 km²) and Rockingham Counties (2.41 – 8.53 coyotes/100 km²) using a spatial capture-recapture model. Six-month …
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A System-Level Analysis of Migratory Shorebird Habitat Use, Diet, and the Influence of Climate-Driven Ecosystem Change in the Virginia Barrier Islands
… (May 14 – June 2, 2023 – 2024) and used fecal DNA metabarcoding to examine diet and prey selection on mudflats. Mudflats supported comparable shorebird numbers and invertebrate densities to barrier island sand substrate. The composition of shorebird and invertebrate communities differed …
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The stool microbiota and infant wheezing illness - the Drakenstein child health study, South Africa
… six months and 12 months. Deoxyribonucleic acid (DNA) was extracted from all the samples using the manual ZR Fecal DNA MiniPrep™ Kit. Sequencing of the hypervariable V4 region of the 16S rRNA gene was performed using the Illumina MiSeq technique. The resulting sequencing data was subjected to …
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African elephant conservation and population genetics
… patterns between nuclear and mitochondrial DNA in African elephants are often incongruent, which has been attributed to sex-biased dispersal and variance in reproductive success. To examine this, we sequenced the mitochondrial genome of two African forest elephants and examined the …
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Molecular characterization of entamoeba histolytica tRNA genes
… the antigen against E. histolytica. Genomic DNA was extracted from 78 stool samples that were positive by ELISA using ZYMO RESEARCH fecal DNA mini Prep kit from inqaba biotech. A multiplex PCR protocol was used for the identification of E. histolytica. Specific primers for the different loci …
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Factors affecting Western Atlantic red knots (Calidris canutus rufa) and their prey during spring migration on Virginia's barrier islands
… We compared these prey availability data to prey DNA data obtained from fecal DNA metabarcoding analyses on red knot feces (n = 100) collected on peat and sand substrates between 2017 – 2019. Red knots consumed prey from Orders Veneroida (clams), Mytiloida (mussels), Diptera (flies), and …
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Conservation of endemic species in China
… individuals and population for giant pandas, fecal bamboo bite size combined with home range analysis and microsatellite analysis of fecal DNA. However, the first one suffers from the lack of accuracy and the latter one is limited by the freshness of the fecal sample and high cost. I developed …
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Ecology of Tigers in Churia Habitat and a Non-Invasive Genetic Approach to Tiger Conservation in Terai Arc, Nepal
… sampling techniques: camera-trapping and fecal DNA sampling. For cameras: I pooled the 2009 camera-trap data from the core tiger population across the lowland areas of Chitwan National Park. I sampled 359 km² of the core area with 187 camera-trap locations spending 2,821 trap-nights of …