Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 13 of 13 for “"fatty acylation"”.
-
Develoment and Application of Chemical Strategies to Study Protein Fatty-Acylation in Eukaryotes
… cells. I showed that alkyne-functionalized fatty acids, in conjunction with azido-fluorophores, provide the most sensitive detection of acylated proteins following CuI-catalyzed azidealkyne cycloaddition. Linkage-specific hydrolysis, mutagenesis and inhibitor studies reveal that these …
-
UNDERSTANDING THE RAL GTPASES: THE REGULATION BY PROTEIN LYSINE FATTY ACYLATION, SIRT2, AND INTERACTING PROTEINS
… and RalB are differentially regulated by lysine fatty acylation. Lipidation, such as cysteine palmitoylation and prenylation, is a key regulatory mechanism for small GTPases. Recently it has been reported that lysine fatty acylation also regulates several small GTPases. My graduate work shows …
-
Bioorthogonal Chemical Reporters Reveal Fatty-Acylation of Histone H3 Variants and Cholesterol Modification of Proteins and Trafficking in Cells
… and tissues. The unbiased proteomic analysis of fatty-acylated proteins using chemical reporters undertaken here revealed a greater diversity of lipid-modified proteins in mammalian cells than had previously been appreciated. Proteins targeted by a series of fatty acid chemical reporters ranging …
-
DHHC PROTEIN ACYLTRANSFERASES CATALYZES THE LYSINE PALMITOYLATION OF MEMBERS OF THE RAS SUPERFAMILY OF SMALL GTPASES
Protein fatty acylation is the attachment of a long-chain fatty acyl group, generally 14–16 carbons in length to the side chain of an amino acid. Cysteine palmitoylation or S-palmitoylation is best understood and widespread post-translational modification in this class, followed closely by …
-
METABOLISM-COUPLED PROTEIN POST-TRANSLATIONAL MODIFICATIONS IN C. ELEGANS AND MAMMALIAN MODELS
… The third chapter focuses on protein fatty acylation in C. elegans, revealing distinct fatty acylation patterns at both amino acid type and protein levels. The monomethyl branched-chain 15-methylhexadecanoylation was found to be the most abundant cysteine fatty acylation. Using its …
-
Characterization and Reconstitution of S-Palmitoylated IFITM3 Antiviral Activity
… understanding of the endogenous levels of S-fatty acylation. While earlier work in our lab has shown this post-translational modification to be critical for IFITM3 activity, it was previously impossible to distinguish between different modified populations. I therefore developed the acyl PEG …
-
Design, Synthesis, and Evaluation of Oleyl-WRH Peptides for siRNA Delivery
… STAT3 gene, herein, we hypothesized to use of fatty acylation with oleyl chain to our newly designed cell-penetrating peptide (WRH) to evaluate their silencing efficiency in breast and ovarian cancer cells. Therefore, the oleyl conjugated (WRH)<sub>n</sub> (n=1-4) peptides were synthesized …
-
Chemical Tools for Exploring IFITM3 S-Palmitoylation and Mechanism
… of proteins in eukaryotes. The detection of fatty-acylated proteins has been challenging but recent advances in chemical labeling methods have enabled more sensitive detection and proteomic analyses, which I summarize in Chapter 1. The proteomic analysis of S-palmitoylated proteins in …
-
Mechanisms of Genome Buffering and Cell Fate Coordination in Adult Tissue Homeostasis
… conserved across animals enforce ω-7 cis fatty acylation of Wnt proteins. Deviant acylation of a Wnt protein using an exogenously supplied trans fatty acid cripples its ability to traverse the secretory pathway due to a previously unappreciated stereoselectivity of the Wnt chaperone …
-
USING CHEMICAL AND PROTEOMICS APPROACHES TO STUDY THE FUNCTIONS OF SIRT6 AND KRAS
… shown to have lysine deacetylation, lysine defatty-acylation and mono-adenosine diphosphate (ADP)-ribosyltransferase activities. However, the contribution of each activity to the various functions of SIRT6 is poorly understood. In Chapter 2, I describe the dissection of functional contribution …
-
Characterization of Bacterial Metabolites Involved in Host Pathogen Resistance
… we use a chemical reporter strategy to analyze fatty acid protein modifications in bacteria. Dietary and bacterially-produced fatty acids can modulate the virulence of various pathogens, such as Salmonella typhimurium, possibly through covalent protein modification. We demonstrate that …
-
Control of Salmonella Virulence by Microbiota-Derived and Dietary Fatty Acids
… virulence. In this thesis, I describe how fatty acids, derived from both gut microbiota and diet, contribute to attenuation of virulence of enteric pathogen Salmonella. In Chapter 1, I review how dietary and microbiota metabolites affect different aspects of host-microbe interactions. These …
-
Enzymology of DHHC-mediated Protein S-Acylation
Protein <italic>S</italic>-acylation is the post-translational modification of proteins with long-chain fatty acids at cysteine residues via a thioester linkage. The most commonly attached lipid is 16-carbon palmitate, thus the process is often called palmitoylation. Unlike other lipid …