Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"fatty acid biosynthesis"”.
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The effects of acetate and malonate on growth and fatty acid biosynthesis in Aspergillus niger
… ten percent glucose or sucrose medium on growth, fatty acid distribution, and incorporation of radioactive carbon sources into lipids using submerged Aspergillus niger cultures.
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Characterization of beneficial mutations in unsaturated fatty acid biosynthesis that are recurrent dead-ends in a long-term evolution experiment with Escherichia coli
… outcomes within these lineages. Decreased fatty acid synthesis (repressed by FabR) during glucose starvation activates the global repressor SpoT to produce the cellular "alarmone" (p)ppGpp, inhibiting cell growth during the stringent response. Thus, it is possible that fabR mutations that …
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The Role of Acyl Carrier Protein Isoforms in Determining Plant Lipid Content and Composition
… a small protein that is a critical cofactor for fatty acid biosynthesis in all organisms. ACP carries growing acyl chains through all of the enzymatic steps in fatty acid biosynthesis. Acyl-ACP's are also the substrate for stearoyl-ACP desaturase and plastid acyltransferase reactions. Multiple …
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The interaction of hepatitis C virus and intracellular lipid metabolism
… containing cells with cerulenin, which inhibits fatty acid synthase, reduced replication of HCV RNA in a dose dependent manner (Su et al., 2002). Polyunsaturated fatty acids (PUFAs) have recently been shown to inhibit replication of a genotype 1b sub-genomic replicon. PUFAs are essential and are …
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Characterization and analysis of genes encoding Escherichia coli acetyl-CoA carboxylase
… catalyzes the first committed step of fatty acid biosynthesis, the synthesis of malonyl-CoA. In Escherichia coli the enzyme is a complex of four subunits that catalyzes two distinct half-reactions.
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Demonstration of novel enoyl-acyl carrier protein reductases in Vibrio cholerae
One of the growing interests in bacterial fatty acid biosynthesis is due to the fact that an increasing number of both synthetic and natural antibacterial compounds specifically target fatty acid biosynthesis enzymes. However, the investigations this approach has engendered have uncovered diversity …
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Total Genomic Transcriptional Analysis of the Fatty Acid Degradation Regulon of Escherichia Coli
… in E. coli and is required for unsaturated fatty acid biosynthesis. The other enzyme necessary for producing unsaturated fatty acids is 3-hydroxy-decanoyl-ACP dehydrase, encoded by the fabA gene. This gene has previously been shown to be positively regulated by FadR. Double fabA(Ts), fadR …
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Improving advanced biofuels production in Saccharomyces cerevisiae via protein engineering and synthetic biology approaches
… and the low efficiency of synthesizing fatty acid derived advanced biofuels. In this thesis, we aim to improve the production of advanced biofuels in S. cerevisiae using protein engineering and synthetic biology approaches. To overcome glucose repression, a cellobiose utilization pathway …
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Activity and mRNA abundance of enzymes for fatty acid synthesis and desaturation in mammary cell cultures
The effect of exogenous unsaturated fatty acids on cellular fatty acid biosynthesis in mammary cells was examined. Under normal situations, even though the diet of a dairy cow contains considerable amounts of unsaturated fatty acids, viz. oleic acid (18:1) and linoleic acid (18:2), the major …
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Exploring the Potential Use of Camelina Sativa as a Biofuel Crop for Nevada
… M3 generation EMS population were evaluated for fatty acid composition. Increased variation in fatty acid composition was observed when compared with the control, wildtype cultivar. Most importantly, lines demonstrated putative decreases in relative linolenic acid content, a desirable outcome for …
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Bioaccumulation of Triclocarban, Triclosan, and Methyl-triclosan in a North Texas Wastewater Treatment Plant Receiving Stream and Effects of Triclosan on Algal Lipid Synthesis.
… coli and Arabidopsis have been shown to reduce fatty acid biosynthesis and total lipid content by inhibiting the trans-2 enoyl- ACP reductase. The lipid synthesis pathway effects of TCS on field samples of Cladophora spp. were also investigated in this study by using [2-14C]acetate radiolabeling …
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Computational investigation of the Plasmodium falciparum fatty acid biosynthetic pathway toward the discovery of novel antimalarials
… are related to its biological function. In the fatty acid elongation cycle, one small carrier protein shuttles and delivers the acyl intermediates from one enzyme to the other. The carrier has to recognize several enzymatic counterparts, specifically interact with each of them, and finally …
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Determination of fatty acid synthesis intermediates in escherichia coli and bacillus subtilis
… investment ensures that the flux through the fatty acid biosynthesis (FAS) and related metabolic pathways is very precisely coordinated. A key feature of the FAS pathway is the acyl carrier protein (ACP), which is a small acidic protein that tethers acyl intermediates via a high-energy …
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On the molecular diversity of dimethylsulphoniopropionate catabolism by marine bacteria
… acrylate as a sole carbon source via a fatty acid biosynthesis route, linked to propionate catabolism.
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Design and Synthesis of Covalent Inhibitors as Novel Anti-Infective Compounds
… warheads with both the normally targeted amino acids, cysteine and serine, and other amino acids identified as being potential targets of covalent drugs (Chapter 2).<br/><br/> A known, non-covalent inhibitor of FabA, an enzyme involved in the fatty acid biosynthesis pathway in Pseudomonas …
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UNCOVERING THE MOLECULAR BASIS FOR FATTY ACID SYNTHASE PRODUCT SPECIFICITY: STRUCTURAL AND KINETIC EVALUATION OF THIOESTERASE I AND THIOESTERASE II
Fatty acid synthase (FASN) is a multifunctional homodimeric enzyme solely responsible for the synthesis long chain fatty acids (LCFAs) de novo. Fatty acids, and their lipid derivatives, are critical components of all cellular biological processes, including energy storage and utilization, membrane …
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The relevance of bacterial isoprenoid biosynthetic pathways for host-microbe interactions
… indicating alterations in inflammation, bile acid profiles and antimicrobial peptide synthesis with implications on community structure of the gastrointestinal microbiota in statin-treated animals. However we found no effect on local Short Chain Fatty Acid biosynthesis (metabolic health marker …
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