Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"fate mapping"”.
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Investigating the Reconstitution and Function of Peritoneal Cavity Macrophages after LPS-induced Peritonitis
… aim of this thesis project was to elucidate the fate of the large peritoneal macrophages after stimulation with an inflammatory agent as well as the eventual return of the macrophage resident cell population. This work used genetically engineered, LPM and monocyte-derived fate mapping mice to …
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Mosaic Analysis with Double Markers (MADM) as a Method to Map Cell Fates in Adult Mouse Taste Buds.
… of precursor cells. In our study, we apply a new fate mapping technique that combines these two ideas. This technique, Mosaic Analysis with Double Markers, allows for simultaneous gene knockout and subsequent tracking of single cells. This allows us to study the potency of precursor cells …
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Recall Germinal Center and Antibody Responses
… employing various prime-boost models in genetic fate-mapping mice, address multiple aspects of the recall B cell and antibody response. First, using cellular fate-mapping in which we mark the primary-cohort B cells and their MBC progeny with fluorescent proteins, we addressed the contribution of …
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THE ROLE OF NOTCH SIGNALING IN THE DIFFERENTIAL ABILITY OF SUPPORTING CELL SUBTYPES TO SPONTANEOUSLY REGENERATE HAIR CELLS IN THE NEONATAL MOUSE COCHLEA
… which is directly responsible for inhibiting HC fate, and was reduced in outer pillar cells and Deiters’ cells, but not in other SC subtypes. From this we conclude that Notch signaling is reduced differentially among SC subtypes. In Aim 2 we investigated whether inhibition of Notch signaling is …
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STEPWISE DIFFERENTIATION OF IL21-PRODUCING FOLLICULAR HELPER T CELLS REVEALS DISTINCT DEVELOPMENTAL AND FUNCTIONAL STATES
… their differentiation are unknown. Here we use fate mapping and reporter strategies to show that, after initial differentiation, Tfh cells transition to an IL-21-expressing Tfh21 stage, which is extrinsically regulated by follicular regulatory T (Tfr) cells. Using single cell RNA sequencing, we …
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Investigating the Fate of Pre-neoplastic Cells in a Mouse Model of Medulloblastoma
… been formally established. To investigate the fate of such cells, the patched (ptc) mutant mouse, a model for the brain tumor medulloblastoma was used. Pre-neoplastic cells (PNCs) are found in most ptc mutants during early adulthood, but only 15% of these animals develop tumors. Although PNCs …
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Molecular Mechanisms and Antigen Receptor Requirements for Lymphocyte Adaptation to Intestinal Tissues
… transcription factor ThPOK. Treg fate-mapping coupled with RNA- and ATAC-sequencing revealed that the Treg program shuts down before an intraepithelial program becomes fully accessible at the epithelium. Ablation of Thpok results in premature acquisition of an IEL profile by mLN …
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The design and assembly of neural circuits for vocal communication in songbirds
… juvenile zebra finch brain were identified by fate mapping using engineered retroviruses. Multiple populations of neural progenitors appear to exist in the juvenile zebra finch brain, and each produces different types of neurons. At least three cell types appear to be added to the postnatal …
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Continuous expression of TOX safeguards exhausted CD8 T cell epigenetic fate
… barrier to reinforce the Tex developmental fate by simultaneously maintaining Tex epigenetic commitment while restraining differentiation into Teff. Manipulation of TOX even after Tex establishment could therefore provide a therapeutic opportunity to rewire Tex biology in settings of chronic …
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Investigating the role of kidney mononuclear phagocytes in immune complex-induced inflammation.
… interactions in recent years, due to advances in fate-mapping technologies and the ability to study cells at a single cell resolution. However, numerous studies carried out in different organs have highlighted the difficulty of drawing parallels between MNPs in different tissues, with …
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Studying Myeloid Cell Heterogeneity After Spinal Cord Injury via Time-Resolved Single-Cell RNA Sequencing
… I repeated the experiment with a transgenic fate-mapping mouse line then integrated these two datasets to generate a time-resolved SCI myeloid cell atlas with definitive ontogeny labelling. With this dataset I generated a putative time resolved map of myeloid cell dynamics across the SCI …
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Neurogenesis and Gliogenesis from Ascl1 (Mash1) Expressing Progenitors in the Central Nervous System
… roles in determining distinct neural cell fates and thus contribute to mechanisms controlling neural cell type diversity during the embryogenesis. Fundamental to understanding nervous system formation is to uncover links between early cell type specification mechanisms, the developmental …
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Methods to Study Antigen-Specific B cells
… (ASCs) and a reporter mouse model that enables fate-mapping of stimulated B cells. Firstly, I introduce a screening platform for studying single ASCs. Studying ASCs with high throughput is inherently difficult due to the problem of linking the secreted antibody to the cell it originated from. To …
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Unraveling the Interaction Between Beige Adipocytes and the Sympathetic Nervous System
… early postnatal and adult beige adipocytes. By fate mapping beige adipocytes through development, we found that the majority of cold-induced beige adipocytes in adult subcutaneous fat arise from existing mature adipocytes that were once early postnatal beige adipocytes. These studies provide …
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HIGH-THROUGHPUT PROFILING OF INNATE IMMUNE CELLS TO UNVEIL TUMOR ESCAPE MECHANISMS IN MATCHED COLORECTAL CANCER AND LIVER METASTASES
… activation and immune escape. By linking clonal fate mapping with transcriptional states, this work provides a mechanistic framework for characterizing early immune influences in synchronous CRLM, reframing the liver not as a passive metastatic destination but as an active architect of immune …