Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"fascin"”.
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Sphingosine Kinase 1 Regulates Fascin Expression to Promote Metastasis In Triple Negative Breast Cancer
<p>Distant metastasis is the primary cause of breast cancer–related mortality. To date, effective therapeutic drugs that target metastasis are still lacking. Triple negative breast cancer (TNBC) occurs in high frequency in young women and are more likely to recur and metastasize than are other …
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Contributions to the analysis of proteins
… crambin. Finally, the structure of Homo sapiens fascin-1, an actin-binding protein that is present predominantly in filopodia, is examined and described in detail. Application of a sequence conservation analysis to the protein indicates highly conserved surface patches near the putative …
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Structural Mechanisms of F-actin Mechanochemical Regulation and Flexible Network Assembly
… configurations. Most recently, I have focused on fascin, the protein primarily responsible for tightly bundling F-actin in filopodia. Our structure of fascin bridging filaments explains the mechanism of action of a fascin inhibitor currently in phase 3 clinical trials as a cancer therapeutic, and …
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The Cytoskeletal Mechanisms of Cell-Cell Junction formation in Endothelial Cells
… such as an internal actin bundle associated with fascin along the length and VASP at the tip. Strikingly, unlike conventional filopodia, transformation of actin organization from the lamellipodial network to filopodial bundle during bridge formation occurs in a proximal-to-distal direction and is …
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Self-assembly of filopodia-like structures on supported lipid bilayers
… base of the structures, and bundling protein fascin to the shaft. Actin subunits assemble at the tip and translocate into the shaft. FLS assembly requires negativelycharged lipid membranes, with specific requirements for PI(4,5)P 2 and, for maximal efficiency, phosphatidyl-serine. The focal …
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A molecular investigation of the novel gene underlying autosomal dominant retinitis pigmentosa in a South African family
… PKCA (protein kinase C alpha) and retinal fascin. These candidates were chosen on the basis of (i) mapping to 17q, (ii) expression in the retina and/or (iii) potential involvement in the rod phototransduction pathway. Recombination events between the adRP locus and a single strand …
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Spatio-temporal properties of membrane-localized actin nucleating complexes
… GTPase-binding domain, Ena, VASP, Diaph3, Fascin) revealed that the FLS tip complex has both dynamic and stable properties, with different proteins displaying distinct dynamics. Further analyses on the membrane-binding protein Toca-1 showed that its dynamic turnover is controlled by …
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Characterizing New Molecules and Mechanisms of Semaphorin/Plexin/MICAL Signaling
… F-actin stabilizing/bundling proteins including fascin and espin in regulating the F-actin cytoskeleton in vivo. This work also indicated that Semaphorin/Plexin/Mical activity not only directly disassembles the F-actin cytoskeleton but also triggers other actin regulatory proteins to reorganize a …
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Structural and biochemical characterization of LINC complexes
… between Nesprin-2 and FHOD1, as well as Fascin-1, both well known actin binders. Additionally, I have initiated the X-ray crystallographic analysis to obtain a detailed structural understanding of these complexes and provide detailed insight into the pathologies associated with aberrant …
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ROLE OF FORMIN FHOD1 IN THE ACTIN PEDESTAL FORMATION
Enteropathogenic E. coli (EPEC) is an extracellular pathogen that causes polymerization of actin at the site of bacterial attachment, referred to as ‘actin pedestals’. Actin polymerization in the pedestal is believed to be regulated via the Nck-WASp-Arp2/3 pathway. Recent in vivo studies in mice …