Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"farnesylation"”.
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Farnesylation-dependent regulation of transcripts by PARP13
… TRAIL-R4 -a pro-survival TRAIL ligand receptor. Farnesylation is a post-translational lipid modification that causes proteins to associate to membranes and is required for the activity of some proteins such as Ras. Farnesyl modification adds a 15-carbon prenyl group to the C-terminus of proteins, …
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EXPANDING THE POTENTIAL PRENYLOME: PRENYLATION OF SHORTENED TARGET SUBSTRATES BY FTASE AND DEVELOPMENT OF FRET-BASED SYSTEM FOR DETECTING POTENTIALLY “SHUNTED” PROTEINS
… of FTase by demonstrating sequence-dependent farnesylation of shorter three-amino acid "Cxx" C-terminal sequences using both genetic and biochemical assays. Surprisingly, biochemical assays utilizing purified mammalian FTase and Cxx substrates reveal prenyl donor promiscuity leading to both …
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Increased Geranylgeranylated K-Ras Contributes to Antineoplastic Effects of Farnesyltransferase Inhibitors.
… be alternatively geranylgeranylated (GG’d) if farnesylation is inhibited but are preferentially farnesylated.</p> <p>Small molecule inhibitors of farnesyltransferase (FTIs) have been developed as a means to alter Ras signaling. Our initial studies with FTIs in malignant and non-malignant cells …
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Characterization Of Protein Prenyltransferases And Protein Prenylation In Plasmodium Falciparum
… P. falciparum compared to the human host such as farnesylation of proteins are sensitive to inhibition by geranylgeranyltransferase inhibitors.. In-silico search of the malarial genome sequence identified potential protein prenyltransferase substrates. One of these substrates is a SNARE protein …
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Role of Zmpste24 in Prelamin A Maturation.
… and methylation reactions which occur after farnesylation of the CAAX-box cysteine, there is a second endoproteolysis that occurs 15 amino acids upstream from the C-terminal farnesylated cysteine residue. Studies with knockout mice have implicated the enzyme Zmpste24 as a candidate to carry …
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Islet Dysfunction In Diabetes
… and a site-specific inhibitor of protein farnesylation [FTI-277], promoted mitochondrial and nuclear defects as evidenced by caspase activation and lamin degradation in INS-1 832/13 cells and normal rodent islets. Our findings also suggest that inhibition of protein prenylation leads to …
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Adaptive Immunity: From Autoimmune Diseases to Neuroinflammation
… deletion of the enzymes that mediate farnesylation and geranylgeranylation (encoded by Fntb and Pggt1b, respectively) leads to a reduction of eTreg cells and the development of fatal autoimmunity. In Chapter 4, we further explored the mechanistic role of protein prenylation in the …
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Studies and applications of protein prenylation using isoprenoid analogues and site-directed mutagenesis
The site-specific enzymatic modification of proteins offers a powerful tool both for the development of new research techniques and for the development of more effective therapeutics. Protein farnesyltransferase (PFTase) is a promising tool in the field of site-specific protein modification due to …
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The Role of ERp57 in Hras Intracellular Trafficking and Function.
<p>Ras is a central player in signal transduction that mediates cellular proliferation and differentiation. Recent evidence has shown that lipid and non-lipid modified domains participate in Ras traffic and that plasma membrane association is mediated by vectorial vesicular transport from the …