Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"extremophile"”.
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Biomass productivity enhancement and extraction of high values products of an extremophile microalga from SCCA culture collection
… Culture Collection of Algae (SCCA), is a green extremophile microalga found in a mine drainage habitat and characterized by the ability to live in highly selective environments with extreme conditions, such as high concentrations of heavy metals. This strain has been already elected for its rich …
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Atvps34, the Arabidopsis Phosphotidylinositol 3-Kinase, Its Functions in ABA and Auxin Signaling Pathways and the Molecular Basis for Salinity Stress Tolerance in Extremophile Thellungiella Halophila
Thellungiella halophila, a close relative of Arabidopsis thaliana, is a halophyte that can survive in sea water. Microarray transcript profiling and intensity analysis, quantitative RT-PCR, and metabolite profiles revealed shared and divergent responses to salt stress in the two species. Sixty …
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High-power acid Biophotovoltaic cells for the generation of green electricity
… generation. This project describes the use of extremophile microorganisms as the biological material in MFCs, for the investigation of low internal resistance biological fuel cells. In particular, this thesis focuses on BPV (biological photovoltaic) cells, a type of MFC that utilises …
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AcidopHiles : a not- so-basic life
… life forms, however, thrive. They are called extremophiles. One subset of extremophile called the acidophile live in acidic environments, at time even more corrosive than battery acid. Acidophiles are microbes, and live together in hugely diverse ecosystems. Each species of acidophile fills a …
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Determination and Dissection of DNA-binding Specificity for the Thermus thermophilus HB8 Transcriptional Regulator TTHB099
… a cAMP receptor protein (CRP)-family TF from the extremophile <em>Thermus thermophilus</em> HB8. Using the <em>in vitro</em> iterative selection method Restriction Endonuclease Protection, Selection and Amplification (REPSA), we identified the preferred DNA-binding motif for TTHB099, …
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The development of Sinorhizobium meliloti and Deinococcus radiodurans as chassis for synthetic biology applications
… symbiont, Sinorhizobium meliloti, and the polyextremophile, Deinococcus radiodurans. While some progress has been made toward the development of these chassis, further strain and tool developments are required to unlock their full potential. Here, I present the expansion of the genetic toolkits …
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Doctoral Research Project : Sustainable production of bacterial polymers
… study investigated the ability of an unexplored extremophile bacterium, named Pseudohalocynthiibacter aestuariivivens P96, to accumulate 78% dcw of P(3HB). This research, published in collaboration with Stazione Zoologica A. Dohrn, marks a significant step towards commercialising PHAs' use of …
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Microbial glycerolipids in the global ocean: environmental controls and sinking flux dynamics
… pack ice floes to gain insights on growth in an extremophile environment. The caloric content of this glycerolipid biomass is additionally calculated to assess its value to higher trophic level consumers within the sea ice ecosystem. I find that glycerolipids’ physical distribution within sea ice …
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REPSA Directed Assessment of Native Cleavage Resistance of DNA to Type IIS Restriction Endonucleases and Modification of REPSA for High Temperature Application
<p>We have modified the combinatorial selection method Restriction Endonuclease Protection and Selection Assay (REPSA) to work in high temperature conditions for the discovery of new DNA-binding proteins in thermophiles (HT-REPSA). We utilized <em>Thermus thermophilus </em>(HB-8/ATCC 27634/DSM 579) …
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Discovery of novel transaminases from halophilic microorganisms
… ineffective. Halophiles are one such group of extremophile, adapted to survive at high salinity. Their enzymes also exhibit a number of other useful properties, such as thermotolerance, alkalitolerance, and tolerance to the presence of organic solvent.<br/><br/> As such,they represent a viable …
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Using PCR Amplification and Genetic Sequence Analysis of 18S rRNA Genes to Survey the Microbial Diversity and Distribution of Eukaryotic Microbes Inhabiting Two Thermo-acidic Streams in Yellowstone National Park, Wyoming
A cultivation-independent approach, sequence analysis of 18S rRNA genes PCR-amplified from environmental DNA, was used to explore the diversity and distribution of eukaryotic microbes inhabiting algal mats in two acidic geothermal streams in Yellowstone National Park. The objectives were to: (1) …