Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"extraembryonic"”.
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The role of inherited determinants and embryonic-extraembryonic cell interactions in models of mammalian embryogenesis
… signalling interactions between embryonic and extraembryonic tissues are indispensable for successful development. The study of early mammalian post-implantation development is challenging due to a lack of accessibility to the embryo as it grows in the uterus. Recent work has focused on …
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A multi-lineage in vitro mouse embryo model derived exclusively from mouse embryonic stem cells
… egg cylinder consisting of the epiblast (EPI), extraembryonic ectoderm (ExE) and visceral endoderm (VE), which give rise to the embryo proper, placenta and yolk sac respectively. By using stem cells derived from these three fundamental lineages, many *in vitro* models have been developed to …
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Illuminating the Black Box: Defining and Modeling Peri-Implantation Human Embryogenesis
… FGF to ensure proliferation of embryonic and extraembryonic tissues, and the emergence of a group of asymmetrically positioned hypoblast cells that express inhibitors of BMP, NODAL, and WNT signaling pathways and act as the anterior signaling center of the embryo. Next, I extended this work by …
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Assessment of chromatin activity in mouse and human tissues
… ectoderm, an embryo-derived tissue, and two extraembryonic tissues, extraembryonic ectoderm and ectoplacental cone. Embryonic ectoderm and extraembryonic ectoderm nuclei possessed comparable levels of DNAse I sensitivity while ectoplacental cone was significantly less DNAse I sensitive. This …
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Analysis of embryonic development in Tribolium castaneum using a versatile live fluorescent labelling technique
… type and become enveloped by extensive extraembryonic membranes. The genetic basis of these processes has been the focus of active research in several insects, especially Tribolium castaneum. The processes in question are very dynamic, however, and to study them in depth we require …
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A Study of Mouse Peri-Implantation Morphogenesis
… by major remodelling events in the embryonic and extraembryonic lineages from tissue level to single cell scale. With successful implantation as requirement for subsequent development, implantation poses a bottleneck stage for embryo survival, still the mechanisms driving peri-implantation …
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Spatial Transcriptome Profiling of Gastrulating Primate Embryos
… formation. I defined genetic marker profiles for extraembryonic lineages such as the visceral endoderm, specifically defining markers for the anterior visceral endoderm (AVE), a signalling centre which drives symmetry breaking in the mouse. Furthermore, I defined marker profiles and analysed …
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Genome-wide search for regulators of the first cell lineage decision in the mouse: a CRISPR/Cas9 endeavour
… embryo proper, being generally excluded from the extraembryonic layer. On the other hand, trophoblast stem cells (TSCs) resemble TE and are restricted to the extra-embryonic lineage. Despite the efforts of several groups, it is still not possible to completely convert ESCs to TSCs. The molecular …
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Pattern and Mechanism of Calcium Mobilization During Embryonic Development in a Viviparous Snake, <em>Virginia striatula</em>.
… expression of calcium transporting proteins in extraembryonic tissues. The pattern of embryonic calcium uptake of <em>V. striatula</em> occurs late in development, during the phase of highest embryonic growth. Calbindin-D28k, Ca<sup>2+</sup> ATPase, and carbonic anhydrase II are expressed in …
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An in vitro model of human yolk sac and epiblast crosstalk
… signalling interactions between embryonic and extraembryonic lineages. Improved embryo culture techniques have recently allowed human embryos to be cultured in vitro past the point at which they would implant in vivo, until the internationally accepted culture limit of 14 days. Nonetheless, the …
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Trophoblast specification and differentiation potential in early human and non-human primate embryogenesis
… layer of the early embryo. Trophoblast is an extraembryonic tissue that gives rise to the placenta, which sustains fetal growth and development. Human embryo implantation and early trophoblast development have remained elusive due to the inaccessibility of early implantation stages. The …
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The Role of HNF4A in Germ Cell Tumor Development
… undifferentiated GCT line is sufficient to drive extraembryonic endodermal gene programs. This endodermal program subsequently utilizes endogenous WNT signaling to grow. Additionally, isoform specific manipulation of HNF4A revealed differential oncogenic potential amongst HNF4A isoforms. This …
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Developing a stem-cell system of embryonic development organised by a morphogen signaling centre
… and coordination from both the embryonic and extraembryonic tissues. These interactions ensures that the tissues become spatially localized within the embryo for the process of gastrulation, a pivotal event whereby the three germ layers: mesoderm, endoderm, and ectoderm are specified. Bone …
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Characterizing the Phosphorylation State of Tie2 using SH2 Domain Fusion Proteins
… in the heart is formed along with embryonic and extraembryonic vasculature, and angiogenesis, which begins after vasculogenesis and results in the refinement and maturation of the branched vessel system. In pathological angiogenesis, tumors expand by releasing pro-angiogenic factors in response …
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Targeted mesenchymal stem cell differentiation from induced pluripotent stem cells for therapeutic application
… differentiation of neural crest stem cells and extraembryonic linage cells towards the respective MSCs populations. Through a comparison of their transcriptome, we were able to not only distinguish the iMSCs from each other as well as their parent cells, but also match them to various tissue …
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Life history evolution of the lizard Sceloporus scalaris: comparisons of lowland and montane populations
… eggs was associated with both the embryo and extraembryonic tissues. These results suggest that the length of egg retention by S. scalaris is not constrained by eggshell thickness, and that the capacity to regulate the water balance of eggs may be an important component of the evolution of …
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Regulation of amnion induction during the human formative pluripotency transition
… a formative like phase in vitro. Amnion is an extraembryonic membrane that supports the embryo by providing protection, nutrient exchange, and signalling cues. Unlike rodent models, in humans, the amnion segregates from the epiblast shortly after implantation; however, this process remains …
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Investigating Wnt3-mediated symmetry breaking and cell fate in engineered gastruloids
… mouse embryos is informed by cues from extraembryonic tissues (Arnold & Robertson, 2009; Rivera-Pérez & Magnuson, 2005). In contrast, aggregates of embryonic stem cells (ESCs) termed gastruloids are able to display symmetry breaking and develop an AP axis in the absence of extraembryonic …
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Esx1 Dosage Impacts Reproductive Fitness: Effects On Viability and Fertility
… on the X-chromosome with expression limited to extraembryonic tissues and testicular germ cells. Here, we examine the effects of increased and decreased Esx1 dosage on placental and testicular development, the role of genetic background on Esx1 function and characterize the human orthologue of …
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Studying early development using droplet single-cell RNA sequencing
… which surprisingly receives contributions from extraembryonic tissue. The fourth and fifth chapters describe my investigation into the role of the genes Tal1 and T (also known as Brachyury) using chimaeric embryos. These genes are essential for proper development, and embryos die when they are …
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