Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"extraction protocol"”.
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Magnetic bead-based DNA extraction and purification microfluidic chip
A magnetic bead-based DNA extraction and purification device has been designed to be used for extraction of the target DNA molecules from whole blood sample. Mixing and separation steps are performed using functionalised superparamagnetic beads suspended in the cell lysis buffer in a circular …
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Treatment of Medication-Induced Osteonecrosis of the Jaws, Using Platelet-Rich Fibrin PRF // Лечение на медикаментозно-индуцирана остеонекроза на челюстите с приложение на богат на тромбоцити фибрин PRF
… MIONJ treatment. Doham et al.(2006) develop an extraction protocol for PRF.
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Efficiency of Using Commercial DNA Extraction Kits and the Organic Extraction Method in Removal of Polymerase Chain Reaction Inhibitors
… different methods, namely the PrepFiler DNA extraction kit, DNA IQ DNA extraction system, and the organic DNA extraction protocol. One, two, five, and ten μl of whole blood were deposited on soil, wood chips, and cotton swatches treated with bleach. DNA was extracted from all samples and …
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Influence of Biosolids upon the uptake of Mn and Cd by Radish (Raphanus sativus L.)
… the characterization of biosolids, sequential extraction of the biosolids, and the determination of the metal content in the root, shoot and leaves of radish (<em>Raphanus sativus L</em>.).The biosolid samples from Nacogdoches Wastewater Sludge (NWWS), Lufkin Wastewater Sludge (LWWS), Soil …
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Eco-Integrated Production and Formulation of Arthrospira platensis Dietary Supplements
… by optimizing cultivation efficiency, enhancing extraction yields of bioactive compounds, and improving bioavailability. Spirulina was cultivated in vertical tubular photobioreactors utilizing mine water supplemented with Zarrouk medium, achieving biomass yields and pigment concentrations …
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Evaluating the use of capillary electrophoresis in the forensic DNA profiling of burnt teeth
… different temperatures, using an optimised DNA extraction protocol. Tooth samples from 25 donors (n = 100 [4 teeth per donor]) were subjected to three burning conditions, one tooth was left unburnt to act as a control and three teeth were each burnt in a muffle furnace at 100 ˚C, 200 ˚C, and 300 …
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Optimisation of DNA extraction from teeth submerged in sea water in False Bay, South Africa
… and possible methods to optimise the DNA extraction workflow. Compounding this problem is the fact that South Africa experiences a high number of unidentified human bodies entering its mortuaries each year, and these individuals often remain unidentified. The aim of the study was to …
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The house dust microbiota in the Drakenstein Child Health Study
… the performance of ten commercial nucleic acid extraction kits on dust samples; 2) optimise dust removal from electrostatic dustfall collectors (EDC); 3) determine the bacterial composition of house dust using 16S rRNA gene sequencing and 4) determine those factors influencing the bacterial …
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Signal-metabolome interactions in plants
… robustness of the metabolite profiling protocol. The recovery of plant metabolites proved to be satisfactory robust against variations in the extraction protocol by using common extraction procedures for phytohormones; a joint extraction of metabolites and hormones from plant tissue …
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A Proteomics Approach to the Examination of Proteins in Marine Systems
… unsuccessful but led to the optimization of an extraction protocol for an added protein standard, bovine serum albumin, from sediments. An investigation into the expressed proteome of the heterotrophic marine bacterium, <italic>Ruegeria pomeroyi</italic>, under environmentally realistic carbon …
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Investigation of protein destabilization in monodisperse poly(lactide-co-glycolide) microparticles
… release study, we have developed a three-step extraction protocol to recover bovine serum albumin (BSA) from PLG microspheres. In this protocol, we first completely extract all water-soluble BSA entrapped within PLG microspheres, followed by any noncovalent then covalent protein aggregates. …
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Nematodes as indicators of sustainable soil health management practices in corn-soybean production
… combined with an improved soil DNA extraction protocol greatly increased nematode detection and taxonomic resolution. The enhanced primer specificity enabled the recovery of nematode assemblages from soil DNA, and was comparable to those from nematode DNA. The direct nematode DNA …
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Expression optimization of a human papillomavirus type 16 therapeutic vaccine candidate in Nicotiana benthamiana leaves
… expression, and to optimize its expression, extraction and purification. This was done by expressing LALF-E7 using different expression vectors, testing different subcellular localizations, and testing the effect of gene silencing suppressors. By using our group's replicating expression …
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Optimization of automated immunomagnetic separation for the detection of escherichia coli 0157: H7 by quantitative polymerase chain reaction
… The major drawback of the technique is prior DNA extraction, which can be time consuming and result in partial loss of target material, followed by small sample input into the qPCR assay. The objective of this study was to develop a sensitive, quick and technically simple sample concentration and …
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Comparative metabolomic profiling of phenolics in the desiccation-tolerant “resurrection plant” Myrothamnus flabellifolia (Myrothamnaceae) using conventional and green chemistry-based solvent systems
… a preliminary green chemistry-based extraction protocol using Natural Deep Eutectic Solvents was also used to further screen for phenolic compounds, and this was compared against the conventional organic solvent system. Several phenolic compounds not previously detected in M. …
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Phosphorus biogeochemistry and models in estuaries: case study of the Southampton Water system
… measured using a modified sequential phosphorus extraction protocol (SPEPs) method. The ranges of the dissolved inorganic phosphorus (DIP) and other nutrients are similar to the data from the SONUS investigations. The overall distribution of DIP are strongly influenced by the sewage inputs to the …
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Development of amoxicillin suppositories and in vitro/ in vivo evaluations
… method was developed and applied to validate the extraction protocol developed by leveraging the solubility and stability of amoxicillin. The developed HPLC method was then used for in vitro and in vivo quantification of amoxicillin. Amoxicillin sodium (AS) 100 mg and 200 mg conventional …
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Abiotic-Stress Application, Global Berry Proteome Profiling, and Cold-Stress Tolerance in Transformed Winegrape (Vitis vinifera L.).
… extremely limited. Using a phenol-based protein extraction protocol, large two-dimensional polyacrylamide gels, and separation of proteins across two overlapping isoelectic focusing (IEF) ranges spanning pI 4-11, a total of 802 proteins were identified from whole grape berries across four …
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Investigation into the Neurogenetics of Vocal Learning in Tursiops Truncatus, the Bottlenose Dolphin
… To this end, we developed a novel brain extraction protocol in which the brain was removed fully intact and preserved in the field, with the left hemisphere placed in chilled formalin and the right hemisphere flash-frozen on dry ice. We utilized this protocol in collaboration with the …
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Evaluation of additive and nonadditive genetic variation in elite maize germplasm for root, shoot, and ear characteristics
… on biological replicates using six different extraction protocols. Analysis of variance was performed on relative metabolite abundances for the 95 metabolites in common across the treatments to detect significant variation for the effect of technical machine error, extraction protocol, and …
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