Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 348 for “"extracellular matrix (ECM)"”.
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Application of Cell-Derived Extracellular Matrix (ECM) Scaffold on Tissue Engineering Cartilage
… evaluate the feasibility of novel cell-derived extracellular matrix (ECM) scaffold on cartilage tissue engineering in vitro and in vivo as nude mouse and rabbit model. Chapter I: A porous cell-derived ECM scaffold was prepared with a freeze-drying protocol using porcine chondrocytes. The ECM …
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The regulation of type I collagen gene expression in stromal fibroblast by breast tumour cells
… Stromal fibroblasts produce most of the extracellular matrix (ECM) components found in the stroma, including type I collagen. Previous in vivo studies in our laboratory have shown that type I collagen mRNA levels are decreased in stage II and III breast tumour tissue compared to adjacent …
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Extracellular matrix gene sequence variant analyses and Achilles tendinopathy
… function of their encoded proteins within the extracellular matrix (ECM) of the tendon.
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CHARACTERIZING THE MICRO-SCALE MECHANICAL PROPERTIES OF HYDROGELS AND CANCER CELLS
… but also to the mechanical properties of the extracellular matrix (ECM).We studied the elasticity of hydrogels formulations frequently used in cell and tissue culturing. A variety of concentrations in a variety of biomaterials, including agarose, alginate, the collagens, fibrin, hyaluronic …
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Matrix-mediated regulation of type 1 collagen synthesis and degradation in cultured fibroblasts
Stromal cells and the extracellular matrix (ECM) components provide the microenvironment that is pivotal for cell growth, motility, attachment and differentiation. Fibroblasts are some of the cells responsible for the synthesis of most of the extracellular matrix proteins. Type I collagen is the …
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An investigation into the genes mediating myoblast migration in the nematode : Caenorhabditis elegans
… This situation creates an environment where the extracellular matrix (ECM) and cell surface contacts are in constant flux, which begs the questions as to how these cells navigate unerringly to their final destination. In an attempt to identify genes mediating these migrations, I performed an RNAi …
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Enhancing functionalities of engineered skeletal muscle tissues by recreating natural environmental cues
… tissue made from muscle cells and the extracellular matrix (ECM). It can be used as a drug testing platform or an implantable tissue, but its practical use has been limited by inferior contractile performance and small size compared to natural muscles. This thesis aims to implement …
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Characterization and Cell-Seeding of Decellularized Adipose Tissue Foams for Wound Healing
… (ASC) delivery system was developed, using an extracellular matrix (ECM)-derived bioscaffold as the platform. Porous decellularized adipose tissue (DAT) foams were fabricated over a range of concentrations, and with different tissue processing methods (mincing vs. milling). These foams were …
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The extracellular matrix of the nematode Caenorhabditis elegans : a model to study the role of cell-matrix interaction in animal development.
This study is to address the role of extracellular matrix (ECM) in animal development. During development, cells have to gain positional or environmental information either from other cells or their environment. The ECM is located in outside of tissue cells and provide environmental information to …
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The redox regulation of alpha 5 beta 1 integrin stimulated MMP production in articular chondrocytes
… to the degradation of the articular cartilage extracellular matrix (ECM). ROS have been shown to serve as secondary messengers in integrin and cytokine signaling pathways which stimulate chondrocyte production of ECM-degrading enzymes called matrix metalloproteinases (MMPs). Fibronectin …
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Laminar Flow-Based Microchemical Systems for Power Generation, Electrochemical Synthesis, and Biological Cell Studies
… covalently immobilized surface gradients of extracellular matrix (ECM) proteins, laminin and collagen 1. These microfluidic platforms are capable of eliciting cell responses such as migration, proliferation, and differentiation, and offer the possibility of in vitro studies of a variety cell …
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Modulation of mesenchymal stem cell invasion into decellularized engineered tissues
… in shaping the organization and alignment of the extracellular matrix (ECM) in developing and mature tissues, where the organization gives the tissue its unique functional properties. This dissertation will discuss research that is fundamental to the understanding of the interaction of cells with …
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Elucidating Structure and Dynamics of Extracellular Matrix Collagen Using Solid-State NMR
… structural information on native cellular and extracellular components at atomic-scale resolution. Fibrillar collagens are the most common component of the extracellular matrix (ECM), comprising up to 20% by weight of the human body and is found in most of the tissues. Due to their diverse …
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Syndecan-4 regulates cell-surface trafficking and biological activity of transglutaminase-2
… isodipeptides. TG2 is externalised in the extracellular matrix (ECM) through an unconventional and not fully understood pathway. Under normal conditions, TG2 modulates cell adhesion, proliferation and tissue repair. Under continuous cell insult higher expression and elevated extracellular …
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PREPARATION AND CHARACTERIZATION OF AN ELECTROSPUN GELATIN/DENDRIMER HYBRID NANOFIBER DRESSING
… dressing was electrospun to mimic the natural extracellular matrix (ECM). Gelatin is biocompatible, biodegradable, non-toxic, and easily available. The antibiotic, doxycycline, has the ability to inhibit matrix metalloproteinases. Matrix metalloproteinases, which occur in excess in chronic …
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Deformation mapping of fibronectin fibrils using digital image correlation
Bone cells produce large amounts of extracellular matrix (ECM) proteins that form a fibrillar scaffold on which bone mineral is deposited. Prior work has shown that fibronectin fibrils are one of the earliest ECM proteins to be assembled. Time lapse imaging techniques show that the deposition of …
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Elucidating Cell Fate Regulation of the Bone Marrow Vascular Niche Through Development of Engineered Extracellular Matrices
… two-dimensional scaffold comprised of several extracellular matrix (ECM) components. HSPCs that were co-cultured on ECM-mimicking scaffolds maintained higher viability in culture for up to seven days in comparison to solo-culture counterparts, and functional tests revealed an improvement in …
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Alternatively spliced isoforms of Fibronectin, Tenascin-R and other potential players in early vasculogenesis
… defects are likely due to alterations in the extracellular matrix (ECM). Therefore, I have conducted this study to investigate the differences in the ECM composition of the yolk sac in the presence and absence of EIIIA and EIIIB. I first collected yolk sacs at E9.5 from wild type, EIIIA-null, …
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Transglutaminase in the life and death of the pancreatic β-cell
… mechanisms, cell-surface associations, and extracellular matrix (ECM) interactions. In the pancreas, the expression of TG may be fundamental to the insulin-secretion function of β-cells, and associated diabetic disorders. The functional roles of TG2 in the pancreas were investigated in the …
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3D Bioprinting Pediatric Trachea
… First, this work focuses on incorporating extracellular matrix (ECM) into bioinks for 3D bioprinting patient specific, tissue engineered constructs. The results discussed in this dissertation show while ECM is a powerful mediator of cell behavior, its efficacy can be inhibited by chemical …
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