Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 28 for “"extracellular loop"”.
-
The role of extracellular loop three of the human gonadotropin releasing hormone receptor in ligand selectivity
The hypothalamic neuropeptide, gonadotropin releasing hormone (GnRH) perferentially inteacts with GnRH type I receptors on the gonadotropes in the anterior pituitary. Activation of the GnRH receptor is required for the biosynthesis and release of luteinizing hormone (LH) and follicle stimulating …
-
Evaluating the Interaction Between the Human Melanocortin-2 Receptor and the Accessory Protein, Mrap1: Chimeric Receptor and Alanine Substitution Studies on Transmembrane Domain 4, Extracellular Loop 2, and Transmembrane Domain 5
… of MC2R and this activation motif located on the extracellular space side of the plasma membrane of the cell. The objective of this dissertation was to analyze potential contact sites between the extracellular space side activation motif of MRAP1 with the extracellular domains of the MC2R—the …
-
Investigations of tetraspanin functions using large extracellular loops
… of a specific tetraspanin domain: the large extracellular loop (LEL). Tetraspanins are involved in cellular migration, adhesion, and metastasis, sperm-egg fusion, and viral infectivity. The large extracellular loop domain is the major extracellular domain of tetraspanins and the binding of a …
-
Identification of Residues Critical to Epithelial Sodium Channel Assembly
… employed to promote random mutagenesis in the extracellular loop of alpha-ENaC. The levels of growth inhibition were monitored in the yeast strain S1InsE4A and subsequently characterized. The location of the point mutations and the corresponding amino acid transitions cause varying degrees of …
-
Uncovering the Critical Amino Acids in the Beta Bubunit of the Epithelial Sodium Channel (ENaC)
… was used to introduce random mutations in the extracellular loop of the beta subunit of ENaC in order to identify residues that may be critical to proper function. A yeast screen, dilution survival pronging assay, was used to visualize the level of growth inhibition of yeast cells expressing …
-
The G-protein coupled receptor CMKLR1/ChemR23: Studies on gene regulation, receptor ligand activation, and HIV/SIV co-receptor function
… to a varying degree in the N-terminus and second extracellular loop, whereas the viral interaction in the case of SIV primarily involves the second extracellular loop.
-
Binding and Signaling Differences between Prostaglandin E1 and E2 Mediated by Prostaglandin E Subtype Receptors
… constrained to a conformation of the second-extracellular loop of human prostaglandin-E2 (PGE2) receptor subtype 3 (hEP3) was synthesized. The contacts between the peptide residues at S211 and R214, and PGE2 were first identified by NMR spectroscopy. The results were used as a guide for …
-
Pharmacological and Molecular Characterisation of P2Y Receptors in Endothelial and Epithelial Cells
… (designated L247), designed to mimic the third extracellular loop of the human P2Y2 receptor, the aim of this study was to use this peptide as an immunogen to generate and fully characterise polyclonal rabbit antibodies to the P2Y2 receptor. Other aims of this study were to characterise …
-
Function and pharmacology of TRPM3 ion channel
… a short and reasonably unique peptide in the 3rd extracellular loop (E3) region, to which polyclonal antiserum (TM3E3) was produced. Extracellular application of TM3E3 inhibited TRPM3 function with a high degree of specificity, having no effect on TRPM2 or example members of other sub-types of …
-
Biogenesis and Traffic of the Potassium Channel
… on two consensus sites in the first extracellular loop. The importance of glycosylation at this location for Shaker biogenesis has not been previously studied. Elimination of the two consensus sites for N-linked glycosylation yields a channel that targets to the ER, integrates and …
-
Characterisation of Rhodopsin Retinitis Pigmentosa mutants located in Intradiscal Loop 1
… the effect of three RP mutants located in Extracellular loop 1 of bovine rhodopsin. Expression levels and protein folding have been examined using HEK 293 GnTI- cells and two methods have been used in an attempt to correct protein folding. The effects of these two correction methods on …
-
The role of C-terminal cysteines in regulating human proteinase-activated receptor-1 function
… that they carry their own tethered ligand within extracellular N-terminus. To activate the receptors, the tethered ligand is exposed by proteolytic cleavage which subsequently binds to the extracellular loop 2 to trigger receptor function. Four members have been identified so far in this …
-
Are bats evolving more potent antiviral immune systems? Investigating the evolution and antiviral capacity of SERINC3 in Rhinolophus bats
… analysis identified the transmembrane helices, extracellular loop 4, and 6 residues in SERINC3 to be possible functional regions and target for future analysis. The results of this study will help give insight into the bat immune system and how bats tolerate chronic viral infection. …
-
Artificial and mammalian olfaction and low-coordinate metalloporphyrin complexes
… utilizing metal ion ligation in an extracellular loop as a primary interaction with odorants. A highly conserved metal ion binding motif, HXXC[D,E], has been found to be present in 62-77% of all mammalian olfactory receptors and an alternative sequence, QXXC[D,E], is present in …
-
Towards Understanding Membrane Protein Folding and Stability with Solid-State NMR and Hydrogen/Deuterium Exchange
… which includes interactions such as folding of loops and binding of ligands. It may play a crucial role in the formation of a functional and stable protein; however, the nature of this stage is yet to be elucidated. In our research, we aim to probe interactions contributing to MP stability …
-
Claudins and tight junction-associated MARVEL proteins at the blood-brain barrier – contribution to paracellular barrier formation
… barrier nor interconnects opposing cells through loop interactions. But a contribution to the proper TJ strand morphology, possibly through interactions with other TJ associated proteins is found. Despite lack of the typical PDZ binding motif, interaction of Cldn25 with the PDZ1 domain of zonula …
-
Structural and functional investigation of the organic anion transporting polypeptide 1B1 (OATP1B1)
… the FLAG epitope was inserted into the putative extracellular/intracellular and terminal regions of the protein. Detection of FLAG antibody bind ing was quantified by Juminometry and visualised qualitatively using confocal microscopy. Results revealed that the pred icted internal N and C termini …
-
Biochemical Characterization of CXCR4 Interactions With HIV -1, Natural Ligands and De Novo Designed Inhibitors
… HIV-1 and SDF-1alpha functional sites on the extracellular domains of CXCR4 has been well documented, it is yet to be determined whether there are sites in CXCR4 important for HIV-1 and/or SDF-1alpha functions and, if such sites do exist, whether they are overlapping or distinctive for the …
-
Drug discovery at class A and class B GPCRs
… at the top of transmembrane domain 2/3 and extracellular loop 1. The second part of this thesis is then focused on drug discovery at adenosine receptors with the aim of developing more selective and more potent compounds. Firstly, compounds were screened for more potent adenosine 1 agonists …
-
The Tetraspanin CD9 Localizes to Platelet-Platelet Contacts and Regulates Thrombus Stability
… which binds with high affinity to the large extracellular loop of CD9. Fab fragments were used to avoid complications of bound antibody crosslinking CD9 with the platelet FcγRII receptor. Confocal analysis of platelet spreading indicated that CD9 perturbation by mFab7 resulted in increased …
Page 1 of 2