Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 117 for “"expression systems"”.
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Construction of inducible expression systems for controlled transfer of Agrobacterium tumefaciens T-DNA in plant transient expression systems /
… resulting in higher biosafety of the transient expression in plants. Our proposed solutions involve an inducible expression of essential Agrobacterium tumefaciens virulence factor virE2 and expression of toxin pemK and antitoxin pemI from bacterial toxin-antitoxin (TA) module pemIK in …
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Expression and characterisation of biopharmaceuticals in heterologous expression systems
… established and optimised for heterologous expression in plants, bacteria and mammalian. The first aim of this thesis was the establishment of a protocol for the expression and purification of the anti-HIV antibody, 2F5, in plant suspension cultures (BY-2). Intact and pure antibody could be …
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Insect expression systems : improving intracellular and heterologous gene expression
Expression systems (ES) in a wide variety of biological systems are used to provide foreign protein. Protein production in mammalian cells is a labour-intensive and expensive process and insect cells have been used as cheaper alternatives. Insect ES are based on two types of vector; viral- and …
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Cell-free expression systems for the construction of artificial cells
Cell-free expression systems are widely used to synthesize proteins for subsequent further characterization, to manufacture potentially useful commercial end products, and to construct cellular mimics in the laboratory. The first part of the thesis explores the feasibility of preparing two of the …
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Determining The Genotype-Phenotype Connection In Synthetic Inducible Gene Expression Systems
<p><strong>Introduction</strong> Gene expression is an important process whereby the genotype controls an individual cell’s phenotype. However, even genetically identical cells display a variety of phenotypes, which may be attributed to differences in their environment. Yet, even after controlling …
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Engineering dual-glycan responsive expression systems for tunable production of heterologous proteins in Bacteroides thetaiotaomicron
… such as diagnostics or intestinal delivery systems for therapeutics. Bacteroides thetaiotamicron (B. theta) is a Gram-negative intestinal anaerobe with potential for the capability to produce functional heterologous proteins within a host intestine. To improve the strength and regulatory …
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Developing and applying transient expression systems to identify and understand gene regulatory elements in plants
In this dissertation, I develop transient expression systems to enable massively parallel reporter assays (MPRAs) in crop models. I apply these transient assay systems to identify, characterize and design plant gene regulatory elements. To allow for the scale of MPRAs, I develop a high-efficiency …
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Cloning and expression of the M-gene from the human coronavirus NL-63 in different expression systems
… The strain placed on economies and health care systems of all countries by these diseases are phenomenal. Although we are familiar with various agents leading to these kinds of infections (e.g. rhino-, influenza-, parainfluenza, human metapneumo-, respiratory syncytial-, adeno- and …
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Investigating "difficult-to-express" monoclonal antibody frameworks in transient and site-specific integration-based Chinese hamster ovary cell expression systems
… investments to mitigate these phenotypes. mAb expression in Chinese hamster ovary (CHO) cells, the production host cell of choice, is a crucial but poorly understood developability characteristic that can be significantly impacted by amino acid sequence, leading to orders of magnitude changes …
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Single copy gene expression system as a tool for the purification of membrane proteins from Pseudomonas aeruginosa
… currently available for their study involves expression systems geared for high levels of expression. In the case of membrane proteins which play a role in clinically relevant activities, such as multidrug resistance, an expression system which does not always reflect biologically relevant …
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Expression of Recombinant Chloroperoxidase
… we report on the development of protein expression systems for chloroperoxidase. Both prokaryotic and eukaryotic expression systems were investigated. Chloroperoxidase was overexpressed in E. coli pET expression system. The expressed apoenzyme was secreted into periplasmic space at an …
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Engineering and use of plant viral expression vectors
Different plant virus-based expression systems were developed and compared using the colorectal cancer antibody CO17-1A and the corresponding antigen GA733-2 as model proteins. Tobacco mosaic virus (TMV) and alfalfa mosaic virus (AlMV) were used to construct the vector backbones of four systems to …
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Characterisation of an expression system for commercial production of proteins / by Lene Jorgensen.
… of this thesis is to characterise a bacterial expression system for recombinant production of proteins with relevance to industry. Recombinant protein expression under control of stationary-phase inducible promoters is characterised, and the expression levels are quantitatively compared with …
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RNA transmission and expression from inert HIV candidate vaccine virus-like-particles
… virus-like-particles (VLPs) produced in various expression systems are potent stimulators of both cellular and humoral immune responses in animal models. The encapsidation of large concentrations of random cellular RNA species is known to accompany the assembly of HIV virus particles. This RNA …
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An investigation into the development of plant-derived vaccines against human papillomavirus type 11 and Cottontail rabbit papillomavirus
… compared the potential of two different plant expression systems.
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Engineering the angiotensin II type 1 receptor for structural studies
… is due to their instability in detergent, low expression levels and the presence of misfolded receptors in many heterologous expression systems. The objective of this project was to engineer the angiotensin II type 1 receptor (AT1R), a human GPCR, to make it suitable for structural studies. It …
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Transient heterologous protein expression in green microalgae
… it is referred to as heterologous protein expression. The baculovirus insect cell culture, yeast, and plants, such as Nicotiana benthamiana, are only a few of the commercial expression systems where this technology has been extensively used. These systems, however, often have disadvantages …
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Voltage-Gated Sodium Channel Activity in Mouse Skeletal Muscle Fibers: Normal Gating and Defects Associated with Periodic Paralysis Mutants
… been extensively characterized in heterologous expression systems. These studies have significantly advanced our understanding of the pathophysiology of these disorders. The in vivo functional consequences on channel activity, however, have yet to be defined. Animal models are now available in …
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Studies on the optimization of expression and purification & Functional characterization of Class C – GPCRs
… current study was focused on the optimization of expression, purification and functional characterization of class-C GPCRs involved in neurotransmission. The first part of the study was aimed at optimizing the expression and purification of the ligand binding extracellular domain (ECD) of rat …
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