Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 234 for “"expression system"”.
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Development of a Versatile Antibody Cloning and Expression System
… communities specialised in antibody production, expression levels of grams per litre have been reached. However, lack of a suitable manufacturing platform, which ensures consistent antibody production, has always been one of the major impediments to the development of recombinant antibody …
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Identifying Potential Agonists Of TRPA1 Using A Heterologous Expression System And Transgenic Mice
Chemical irritants stimulate the trigeminal nerve through many different receptor proteins, including TRP channels. TRPA1, a highly conserved TRP channel, is known to be activated by over ninety compounds. As TRPA1 is promiscuous in nature, it was considered a likely receptor for stimuli activating …
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Characterisation of an expression system for commercial production of proteins / by Lene Jorgensen.
… of this thesis is to characterise a bacterial expression system for recombinant production of proteins with relevance to industry. Recombinant protein expression under control of stationary-phase inducible promoters is characterised, and the expression levels are quantitatively compared with …
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Evaluation and simulation of a harvesting and juice expression system for sweet sorghum
… and evaluate a computer program to simulate a system developed by the Agricultural Engineering Department at Virginia Polytechnic Institute and State University to harvest, transport and express juice from sweet sorghum for syrup production. Time study and product data were taken for …
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Enantioselective Epoxidation of Alkenes by Chloroperoxidase and the Development of a Chloroperoxidase Expression System
… and ongoing research in the development of an expression system for chloroperoxidase could open the door for a number of experiments concerning the mechanism of the epoxidation reaction. Also the possibility of improving the utility of chloroperoxidase in asymmetric epoxidations through …
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De novo synthesis of high purity CD3 epsilon peptides utilizing SUMO expression system in bacteria
… the SUMO (Small Ubiquitin-related Modifier) system from yeast, CD3ε1-27 amino acid and a truncated version 2-27 amino acid peptide are expressed in E. coli cells with an SMT3 (Mitotic Fidelity Gene 3) tag. Subsequently, SMT3 tag is removed with SUMO protease and the resulting peptide is …
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Engineering a cyanobacteriochrome two-component system into a synthetic, light-controlled gene expression system for plants
Our inability to perturb the expression of genes at cellular resolution in planta has hampered our investigation of spatial and temporal dynamics. Ideally, we would like an orthogonal, genetically encoded system, that allows us to reversibly perturb the expression of genes of interest with maximal …
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Single copy gene expression system as a tool for the purification of membrane proteins from Pseudomonas aeruginosa
… currently available for their study involves expression systems geared for high levels of expression. In the case of membrane proteins which play a role in clinically relevant activities, such as multidrug resistance, an expression system which does not always reflect biologically relevant …
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Development of a Cytochrome P450 Expression System with Biomass Scale-up, Streamlined Downstream Processing and Stability Modifications
… hepatocytes. In this thesis, a recombinant P450 expression system in yeast was developed with the aim of providing a cost-effective way of producing human P450 enzymes upon the yeast’s endoplasmic reticulum (ER). These ER-bound P450s are usually referred to as microsomal P450s. A yeast expression …
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The development of a flagellin surface display expression system in the gram-positive bacterium, Bacillus halodurans Alk36
… however, restored through complementation of the expression vector carrying a functional hag gene. Polylinkers were inserted as in-frame, chimeric, flagellin sandwich fusions in order to identify the permissive insertion sites corresponding to the variable regions of the flagellin protein. …
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Gene regulation in baculovirus-infected insect cells : application in the development of a budded virus free expression system
The baculovirus expression vector system (BEVS) is a valuable tool for the production of recombinant proteins including complexes such as virus-like particles (VLPs) for vaccine production. A significant limitation of the BEVS, however, is the co-production and subsequent contamination of VLPs and …
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Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System
… In this study we demonstrate the successful expression of a PpcA homolog in the soluble fraction of Escherichia coli. We purified the recombinant protein to homogeneity. This development provides the means to study the enzyme's biochemical properties and manipulate the primary sequence in …
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Taraxacum officinale as an expression system for recombinant proteins : molecular cloning and functional analysis of the genes encoding the major latex proteins
… useful promoters to drive high level transgene expression in genetically engineered T. officinale plants, which would allow a cost-effective purification of recombinant proteins from latex. After determining the partial amino-acid sequences of the MLP cyanobromide peptide fragments, we reported …
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Creation of a Binary CuSO4 Inducible gene expression system in Arabidopsis thaliana and its application to the degradation of Lignin in planta
… approaches to chemically induce the overexpression of lignin degradation enzymes in planta within the model organism Arabidopsis thaliana and deliver them to the cell wall for controlled lignin degradation in a proof-of-concept study.
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Modification of a plant-based expression system to produce Lysosomal Acid Lipase (LAL) in plant cells using genetic modification of endogenous plant N-glycosylation
… of complex N-glycosylation they are a valid expression system to produce safe and correctly glycosylated human proteins. To modify plant glycosylation patterns towards fully human patterns, the plant glycosylation machinery requires substitution of a series of plant enzymes with human ones. …
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Investigation of a novel intein-based Escherichia coli expression system for human methylmalonyl CoA mutase : a thesis presented to Massey University in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry
… CoA mutase for x-ray crystallography, an expression system for recombinant hMCM is required. Other researchers have expressed hMCM in Escherichia coli as (i) insoluble inclusion bodies, (ii) soluble fusion protein that cannot be separated efficiently from the fusion tag, or (iii) in low …
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Expression of Recombinant Chloroperoxidase
… we report on the development of protein expression systems for chloroperoxidase. Both prokaryotic and eukaryotic expression systems were investigated. Chloroperoxidase was overexpressed in E. coli pET expression system. The expressed apoenzyme was secreted into periplasmic space at an …
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Facial expression recognition for a sociable robot
… of humans, we need to develop a robot system that can recognize the emotions of the people it interacts with and can respond to them accordingly. In this thesis, I present a facial expression system that recognizes the facial features of human subjects in an unsupervised manner and …
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Engineering dual-glycan responsive expression systems for tunable production of heterologous proteins in Bacteroides thetaiotaomicron
… such as diagnostics or intestinal delivery systems for therapeutics. Bacteroides thetaiotamicron (B. theta) is a Gram-negative intestinal anaerobe with potential for the capability to produce functional heterologous proteins within a host intestine. To improve the strength and regulatory …
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