Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"expression strategy"”.
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Characterization of grapevine vein clearing virus expression strategy and development of caulimovirus infectious clones
… 7,332 and 7,672 was sufficient to drive expression of downstream ORF. 5' RACE and 3' RACE revealed that transcription was initiated predominantly at nucleotide 7,571 and terminated at nucleotide 7,676. Additional transient expression analysis studies were supportive of a ribosomal shunt …
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Recombinant Engineering Strategies for the Production of Therapeutic Coagulation Factor Proteins
… transgenic cell lines. This resource-intensive strategy must be carried out for each new protein of interest, a critical limitation for complex multi-protein cocktails, and can yield proteins with non-human post-translational modifications; a complication that can lead to reduced protein …
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Nuclear Localization of Proteins and Genome Editing in the Oomycete Phytophthora sojae
… and simplification of the CRISPR/Cas9 expression strategy in P. sojae as well as methods for mutant screening. I also describe several optimized methodologies for P. sojae manipulation based on my 5 years of experience with P. sojae.
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Manipulation of the Rift Valley Fever Virus M-Segment for Vaccine Development
… rMP-12 mutants with altered levels of Gn expression due to point mutations and truncation of the initiation codons. Truncation of the preglycoprotein region up to the 2nd initiation codon resulted in relative Gn expression levels that were almost 3-fold greater than the parental plasmid. …
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Beyond bottlenecks: expression of complementary enzymes and permeabilization of cell membranes to improve performance of CYP153A6 in Escherichia coli whole cell biocatalysis
… the whole-cell biocatalyst include insufficient expression of the CYP153A6 or its redox partners, leading to a lack of biocatalytic “active sites”; lack of oxygen for biotransformation and cell metabolism; poor transport of n-octane from the organic phase into the cytoplasm, leading to …