Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 9 of 9 for “"expression screen"”.
-
A Mis-Expression Screen Identifies a PKA -Anchoring Protein in Antenna Lobe Development
One of the genes recovered was the Drosophila A Kinase anchoring protein 200 (Akap200). This thesis discusses the further characterization of this gene. Akap200 is a Protein Kinase A (PKA) binding protein with multiple binding sites for other signaling molecules, including Protein Kinase C (PKC), …
-
Using Tumour Evolution to Understand the Epigenetic and Transcriptional Adaptations of Cancer to Host Immunity
… was investigated. The results suggested that expression of T cell inhibitory molecules, a reduction in inflammation, and reduced expression of L1 transposable elements may be involved in immune escape in immunoedited tumours. An over-expression screen was performed to attempt to identify key …
-
Functional Roles of Nova in RNA Metabolism
… These considerations prompted a gene expression screen aimed to identify differences in the pool of mRNAs associated with heavy polysomes in wild type and Nova-1-null mice. Several genes whose mRNAs have been found to undergo changes in abundance specifically in the polysome fraction …
-
A Role for BDNF-Trkb Signaling in the Modulation of Superoxide Dismutase-1 Expression
… Our lab has previously demonstrated, via a cDNA expression screen, that the TrkB receptor and the guanine nucleotide exchange factor, RasGRF-1, modulate SOD1 expression at the protein level, suggesting that cell signaling pathways associated with TrkB signaling are involved in regulating the …
-
Biochemische und molekularbiologische Charakterisierung von Spike, einem neuen BH3-Domänen Protein
… genes by using a novel genetic expression screen (Grimm and Leder, 1997). One of the identified genes turned out to be a evolutionary conserved cDNA that codes for a novel BH3-only protein of 219 amino acid residues which was named Spike, for Small protein with inherent killing …
-
Roles of Class II Histone Deacetylases in the Cardiovascular System
… regulate MEF2-HDAC interactions. A eukaryotic expression screen revealed a remarkable variety of signaling pathways that converge on the signal-responsive phosphorylation sites in HDAC5, thereby enabling HDAC5 to connect extracellular signals to the genome. Microarray analysis was performed to …
-
Development of a high throughput screen for the expression of membrane proteins and their purification and crystallisation
… to be inserted into the membrane, recombinant expression yields are often low. In order to obtain enough protein for purification and crystallisation studies, the expression of membrane proteins requires screening for the best expression conditions. Purification of membrane proteins requires, …
-
Developing nanobodies to stabilise the tumour suppressor protein p16INK4a
… project focused on generating and optimising the expression and purification of p16 constructs prior to immunisation of animals to raise nanobodies. A high-throughput approach was taken to generate forty-five different p16 constructs with a range of different solubility and purification tags. …
-
Anteriore Musterbildung im Wirbeltierembryo - Die Induktion von Vorderhirn und Herz
… Identitäten im Wirbeltierembryo haben, wurde ein Expressionsscreen mit Genen aus einer cDNA-Bibliothek aus Gewebe der prächordalen Platte aus Huhnembryonen des Kopffortsatzstadiums durchgeführt. Innerhalb dieses Expressionsscreens wurde eine Synexpressionsgruppe gefunden, deren Mitglieder im …