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Showing 1 to 20 of 24 for “"expression library"”.
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Implementing BREeze - a High-Performance Regular Expression Library Using Code Generation with BuildIt
Regular expression matching is a very common problem in software engineering, with applications in text processing, text searching, data scraping, syntax highlighting, deep packet inspection in networks, etc. Due to the varying complexity of regular expressions, having one general approach to match …
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Analysis of AUF1 targeted mRNA sequences
… AUF 1, mRNA derived from a human cardiac cDNA expression library was purified by AUF 1 affinity chromatography and cloned following RT-PCR. 261 sequences were obtained. The sequences were searched against two protein databases and four nucleic acid databases, and the sequence information and …
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Isolation and partial characterization of cDNAs encoding polypeptides that interact with the capB gene products of Dictyostelium discoideum
… used to screen a Dictyostelium discoideum cDNA expression library to identify proteins that interacted with p34/31. The clones were tested to eliminate false positives. DNA sequencing analysis revealed that the cDNAs isolated encoded: p34/31; CABP1B, the smaller product of capA; TopA, a …
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MOLECULAR BIOLOGY OF HEPATITIS B VIRUS DNA-CELL INTERACTION
… here as E2BP, was isolated from a ?gtll expression library constructed from the hepatoma cell line HuH-6 which was screened with a binding site probe derived from EII. Sequence analysis of E2BP revealed 86.6% homology with a rat hnRNP-C c-terminal protein sequence while conformational …
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Screening for kinases regulating subcellular localization of clock proteins using human kinome library
… and cloning 6 B. Establishment of human kinome expression library 9 C. Cell culture and transfection 9 D. Fluorescence microscopy 11 E. Quantitative real-time RT-PCR 12 F. Cell lysis 2 G. Western blotting 13 H. Immunoprecipitation (IP) and λphosphatase treatment 14 I. Fly genetics 15 J. …
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Identification of a protein kinase substrate in Sulfolobus solfataricus P2
… can lead to disease processes. A λ-phage expression library was generated from S. solfataricus genomic DNA. The immobilized expression products were probed with a purified protein kinase, SsoPK4, and radiolabeled ATP to identify potential native substrates. A protein fragment of the ORF …
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Molecular properties of aspartate transcarbamoylase and related enaymes from wheat
… from wheat seedlings and screen a wheat cDNA expression library to ATCase sequences. Western blotting failed to show any immunoreactive species in extracts prepared under conditions which suppressed protease activity (SDS, -mercaptoethanol), although a low molecular weight (approximately 3.7 x …
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Identifizierung von Cyclin L2 und des Spleißfaktors SF3B1 als Substrate der Proteinkinase DYRK1A
… By phosphorylation of the proteins of a cDNA expression library with DYRK1A, eight in vitro substrates were identified. Two of them, cyclin L2 and SF3B1, were chosen for further investigations. Cyclin L2 combines a cyclin domain with an arginine-serine-rich (RS)-domain and was extensively …
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Facilitating High Performance Code Parallelization
… for a pattern using Java’s standard regular expression library, and replaces it with our own data parallel implementation using Java bytecode injection. Our approach facilitates parallel processing on different platforms consisting of shared memory systems (using multithreading and NVIDIA …
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Molecular analysis of glycogen phosphorylase-1 gene expression during the development of dictyostelium discoideum
… phosphorylase-}, gp-l, was isolated from a λgtll expression library made from amoebae stage mRNA. The 5' upstream region of the gp-l gene was cloned by inverted polymerase chain reaction (IPCR) and partial genomic DNA library screening. The gp-l gene was found as one copy or low copy number gene …
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Aphid Transmission, Replication, and Transcription of BYDV -Pav -Il and Genome Organization of RhPV
… can be effective tools for the analysis of the expression and replication of the BYDV-PAV genome. A Rhopalosiphum padi cDNA expression library was screened to identify aphid proteins that interact with the BYDV-PAV-IL capsid proteins using the yeast two-hybrid system. Seven clones activated the …
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Characterization of Pituitary Protein Expression Patterns During Stages in the Reproductive Cycle of Turkey Hens
… $1.5 million dollars per year. Overall protein expression generated by tissues of the hypothalamic-hypophyseal complex, namely the anterior pituitary, of the mature turkey hen have a profound impact on reproductive cycling (Scanes, 2000). One of the key physiological factors produced by the …
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Identification and Characterization of a Calmodulin -Binding Protein Related to a Family of ATPases Involved in Cell Division and Membrane Fusion
… An Arabidopsis thaliana L. flower cDNA expression library was screened for CaM-binding proteins with 35S-labeled CaM. A partial cDNA whose encoded protein shares a high level of similarity with yeast CDC48p was isolated. A genomic clone was isolated using the partial length cDNA clone as …
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Biochemical characterization of calmodulin isoforms and identification of calmodulin-binding proteins in Arabidopsis
… First, CaM isoforms produced from E. coli expression systems were used to test their abilities to activate pea NAD kinase. There was a small, but significant difference among the CaM isoforms' ability to activate pea NAD kinase in vitro. Minor differences in K$\sb{0.5}$, but not V$\sb{\rm …
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Isolation and characterization of the messenger RNA and the gene coding for a proline-rich zein from corn endosperm
… the total in vitro translation products. A cDNA expression library was constructed by reverse transcription of total poly(A)⁺ mRNA using pUC8 plasmid as vector and <i>E. coli</i> strain DH1 as host. The library was screened for the expression of gamma-zein and alpha-zein by specific antibodies. …
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Investigation of a Possible Multi-enzyme Complex Involved in Nicotine Biosynthesis in Roots of Tobacco (Nicotiana tabacum)
… we used this antiserum to screen a tobacco cDNA expression library. Unexpectedly, two previously unreported genes with strong homology to members of a gene family encoding S-adenosylhomocysteine hydrolase (SAHH) in N. sylvestris and a gene encoding SAHH in N. tabacum were cloned instead. SAHH is …
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Cloning and molecular characterization of vffl gene coding forisomes of vicia faba
… specific oligonucleotides were designed for library screening. In addition, polyclonal Forisome-specific antibodies were obtained. First attempts to identify Forisome-encoding genes were performed by hybridization screening of subtracted and non-subtracted Vicia faba derived cDNA libraries, …
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Identifizierung und Charakterisierung von Interaktionen der Nichtstrukturproteine NS1 und NS2 des Respiratorischen Synzytialvirus mit Proteinen der Wirtszelle
… in the cytoplasm as well as in the nucleus. Expression of an enlarged NS1 fusion protein, EGFP-NS1-BRSV N, resulted in the same nuclear and cytosolic localisation indicating that nuclear localisation is not due to diffusion but rather to an active transport. Thus, NS-Proteins should have …
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Isolation von Ascosporen des pflanzenpathogenen Pilzes Venturia inaequalis und Charakterisierung von Antikörperinteraktionen zur Entwicklung eines immunogravimetrischen Sensors
… the mouse serum, we isolated a gene from a cDNA expression library, derived from mycelia of Venturia inaequalis. The sequence of the cDNA was determined. By means of PCR, the gene was amplified from the genome and also sequenced. No introns were found. The gene encodes a protein of 31 kDa showing …
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Cloning and characterization of a single-stranded DNA-binding protein of Leishmania major
… site of protein-DNA interaction, a A. gtl 1 expression library ofL. major was screened with a radio labelled oligonucleotide probe to detect clones expressing functional DNA-binding proteins. A gene was isolated which encoded a novel DNA-binding protein, referred to as HEXBP. The deduced …
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