Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"exponential amplification"”.
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Ultrasensitive DNA aptasensors based on exponential amplification reaction
… by coupling them with an isothermal DNA amplification technique known as exponential amplification reaction (EXPAR). The method involves two steps: (1) first, the target-induced structure-switching of DNA aptamers causes the release of trigger oligonucleotides from the aptamers …
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The Selection of Aptamers to CD20 and Their Application as Inhibitors of Complement Dependent Cytotoxicity
… as SELEX (systematic evolution of ligands by exponential amplification). Aptamers rival antibodies in both binding affinity and specificity. We developed a novel CD20 specific SELEX method, using a lentiviral system to transfect CD20 cDNA into HEK293 cells. Selection using CD20+HEK cells …
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Supernova-driven turbulence and magnetic field amplification in disk galaxies
… medium. Yet, their effect on magnetic field amplification in spiral galaxies is still poorly understood. Analytical models based on the uncorrelated-ensemble approach predicted that any created field will be expelled from the disk before a significant amplification can occur. By means of …
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Gene expression microarray analysis of small, purified cell subsets
… RNA for direct microarray studies without RNA amplification. We found that both linear and exponential amplification was capable of producing enough RNA for microarray analysis even from a single cell. Both methods distorted the GEP, however, with linear amplification much fewer genes were …
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The Development of an Aptamer-based Surface Plasmon Resonance (SPR) Sensor for the Real-time Detection of Glycated Protein
… beads-based Systematic Evolution of Ligands by EXponential amplification process (MB-SELEX). The experimental protocol of the in vitro selection process have been standardized. The developed aptamers have shown a common pattern in sequence and a similarity in secondary structures. Furthermore, …
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The formulation and refinement of a polymerase chain reaction (PCR) assay for early diagnosis of paediatric HIV infection and genetic analysis of variants involved in vertical transmission of HIV-1
… which allows the identification, and subsequent exponential amplification of low levels of specific viral nucleic acid using specific oligonucleotide primers. A major aim of this study was to develop and instigate a (PCR) assay for the early diagnosis of HIV infection in infected infants. This …
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Novel approaches to the low-cost, portable and rapid detection of bacterial pathogens in foods and food-processing environments
… settings. Isothermal methods for nucleic acid amplification, such as recombinase polymerase amplification (RPA), may have considerable advantages as alternatives to PCR. RPA results in exponential amplification of nucleic acids and operates at a constant, near-physiological temperature (~40°C), …