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Showing 1 to 20 of 191 for “"explants"”.
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Efficiencies of Mass Encapsulation in Alginate of Vegetative Explants
… resulted in a decrease of contamination on explants without a reduction in the number of shoots produced. This would reduce the amount of time and labor on the encapsulation procedure, as no rinsing is required. The best result, looking a nutrient formulation, was found to be WPM with 1 µM …
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A Comparison of Growth of Primary Mouse Marrow Explants
The growth of cells in vitro is a technique which presents many advantages for use in biological research, particularly in the fields of genetics, physiology, and biochemistry. It is possible to follow the metabolic and chemical actions of the cell in a controlled manner since an isolated …
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Peroxidase, Protein, and Growth Responses of Cultured Tomato Pith Explants
Tomato pith explants that were cultured on a simple semi-solid medium were analyzed for changes in peroxidase activity, protein, and growth during a seven day period. Changes in the soluble peroxidase fraction were similar to those observed previously in wounded intact tissue. Protein synthesis, …
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Inhibitory Control of Neural Differentiation in Explants of Rana Pipiens Gastrula Ectoderm
Made available in DSpace on 2014-12-04T22:46:13Z (GMT). No. of bitstreams: 1 6003902.pdf: 2144297 bytes, checksum: 284a504d8631cde70d658a32de811242 (MD5) Previous issue date: 1960
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Callus Development and Organogenesis in Cultured Explants of Cowpea (Vigna unguiculata (L.) Walp
… Embryogenic callus was induced from cowpea explants cultured on MS medium supplemented with dicamba and picloram. Embryogenic suspension cultures were initiated from callus induced on MS supplemented with 3.0 mg/L dicamba or picloram and conditions for maintenance of embryogenic suspension …
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Origin and development of floral buds in tobacco explants: organization of microtubules and microfibrils in differentiating cells
Contains fulltext : mmubn000001_078176832.pdf (Publisher’s version ) (Open Access)
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Cartilage mechanobiology and transcriptional effects of combined mechanical compression and IGF-1 stimulation on bovine cartilage explants
… IGF-1 and static compression in cartilage explants. Discussion: Clustering analysis revealed five distinct groups. TIMP-3 and ADAMTS-5, MMP-l and IGF-2, and IGF-1 and Collagen II, were all robustly co-expressed under all conditions tested. In comparing gene expression levels to previously …
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In vitro milk protein secretion by explants of Holstein bull mammary tissue from two different genetic lines
… hydrocortisone, and bovine prolactin (PRL). Explants in duplicate multi-well culture dishes were incubated in the presence of 3H-Iabeled amino acids or 3H-Iabeled thymidine. Measurements made included: casein in media and homogenized tissue, alpha-lactalbumin in homogenized tissue, …
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Differential effects of progestogens on HIV-1 replication and host gene expression in primary PBMCs and cervical tissue explants
… genes was investigated in cervical tissue explants from premenopausal, HIV-1negative, and contraception negative women and peripheral blood mononuclear cells (PBMCs) from women, by real time quantitative PCR, western blotting and Luminex assays, in response to physiologically relevant doses …
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Peptides as amino acid sources for the synthesis of secreted proteins by mammary tissue explants and cultured mammary epithelial cells
… synthesis of secreted proteins. Mammary tissue explants from lactating (10 to 11 d) CD-1 mice and cultured bovine mammary epithelial cells (MAC-T) were used as experimental models. Explants and cultured cells were incubated at 37°C in a humidified atmosphere of 90% air/10% CO₂ and 95% air/5% …
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The effect of the growth retardant Paclobutrazol on the in vitro growth and development of Betula and Populus species
… eight to twelve week culture period, after which explants were harvested. At harvest, explants of both species were removed from their culture universals after growing on hard agarose gel media containing a range of PBZ concentrations. Explant fresh weight, height and dry weight was recorded. …
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Μελέτη της συναπτογενέσεως του ακουστικού φλοιού in vitro άνευ και κατόπιν ηλεκτρικού ερεθισμού ανάλογου προς τα ηλεκτρικά δυναμικά ενέργειας του κοχλιακού νεύρου
… of the rat's acoustic cortex were studied in explants cultured in vitro, exposed to a continuous sound of a certain frequency. The morphological findings were correlated morphologically with explants of the same area of the cortex from the same animals cultured in vitro under usual conditions, …
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Influence of hormones on synthesis and secretion of milk proteins by mammary tissue from male and female cattle of beef and dairy breeds
… Among cultures of bull mammary tissue, Angus explants secreted greater overall quantities of 3 H-TP and 3H-Alac than Holstein explants (p<.05). Secretion of Alac was also greater in Angus cultures at two of eight treatment periods (p<.01). Concentrations of all three protein fractions were …
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Predicting Articular Cartilage Constituent Material Properties Following In Vitro Growth Using a Proteoglycan-Collagen Mixture Model
… and unconfined compression data for native explants and explants cultured in insulin-like growth factor-1 (IGF-1) and transforming growth factor-β1 (TGF-β1). Results indicate that culture in IGF-1 results in a weakening of the COL fibers compared to native explants, and culture in TGF-β1 …
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Human leptomeninges; inflammatory roles in motor neuron disease, and their potential in gene therapy
… thesis presents post-mortem human leptomeningeal explants as an ex vivo model to explore human leptomeninges. The suitability of the model was established by assessing cell composition and tissue health. Cell markers identified four specific cell populations in the intact explants expressing IBA1 …
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A tissue engineering strategy for integrative cartilage repair
… proteoglycan synthesis was elevated in cartilage explants into which KLD pre-mixed with HB-IGF-1 had been cast; both the explants that had received the enzyme pre-treatment and those that were left untreated had a two-fold increase compared to the explants exposed to non-functionalized KLD. …
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In vitro propagation of Betula uber (Ashe) Fernald
Dormant bud and actively growing shoot tip explants of B. uber were cultured on Murashige & Skoog (MS) and modified woody plant (WPM) basal salts supplemented with organic nutrients and benzylaminopurine (BAP). Shoot tip explants were morphogenically more responsive than dormant bud explants. The …
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A collagen-GAG matrix for the growth of intervertebral disc tissue
… into the matrices was assessed by culturing AF explants on top of or between matrices. Cells were able to migrate up to 1 cm from the explant, implying that AF defects of this size could be filled with CG matrices. In the third experiment, explants and explant-matrix constructs were cultured in …
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Ex vivo and in vitro effects of omega-3 fatty acids on joint health in sows and gilts
… inflammatory response of stimulated cartilage explants from gilts; second to characterize the effects of dietary PUFA supplementation on bone, cartilage, and synovial fluid in sows and gilts. For the first objective, cartilage was obtained from the humeral-ulnar joints of Yorkshire x Landrace …
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