Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"explanted"”.
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The Application of BioHeat Perfusion Sensors to Analyze Preservation Temperature and Quantify Pressure Ischemia of Explanted Organs
… monitor and measure local tissue perfusion of an explanted organ. A total of 12 experiments were performed (6 at a set fluid temperature of 15°C, and 6 at 20°C). All system data was collected, statistically evaluated and finally compared against blind histological readings (taken at the …
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On the Design, Modeling, and Control of a Hybrid Pump System for Dynamic Pressurization of Explanted Mammalian Hearts
A hybrid electromechanical pump system is proposed to mimic left ventricular blood pressure in a living mammalian heart. The system consists of a gear pump and voice coil actuated diaphragm (VCAD) pump connected in parallel. By combining a high-capacity, low-bandwidth gear pump with a low-capacity, …
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Human embryonic stem cells for genotoxicity testing
Established mammalian cell lines and primary explanted cells commonly used in vitro to analyze the genotoxic potential of various agents, often poorly reflect human physiology. Lack of standardization also limits use of primary explanted cells. Human embryonic stem cells (hESCs) have been validated …
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Bovine embryo microinjection, culture, microsurgery, and DNA analysis by the polymerase chain reaction technique
… oviductal epithelial cells (OEC; n=38), or in explanted immature mouse oviducts (n=54). Of the embryos that cleaved once, 52.6, and 30.4 and 0.0% developed to morulae/blastocysts after culture in OEC, TCM-199, and explanted mouse oviducts. Mean cell number for embryos cultured in OEC (24.5) was …
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Ultrasonography Evaluation of Patency of Implanted Infra-Renal Vascular Grafts in the Rat Model.
… weeks 4, 8 and 12 post operatively. Grafts were explanted if there was any ultrasonographic evidence of occlusion or at twelveweek termination of the study. Explant was preceded by angiography and followed by histological assessment of the grafts for patency. Results: Three of the looped and all …
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Epithelial cell plasticity in chronic liver disease (MASLD/NAFLD)
… immunostaining analyses on tissue section from explanted liver to confirm the presence of cells displaying both cholangiocytes and hepatocytes markers thereby reinforcing the transdifferentiation hypothesis. I then use single nuclei RNA sequencing analysis from liver biopsies to capture and …
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Verification of the <i>Ex Vivo</i> Thiel Embalmed Model for Liver Treatment with Magnetic Resonance-guided Focused Ultrasound (MRgFUS) before Clinical Practice
… treatment before clinical practice, by using explanted organs and whole cadavers. The first set of experiments involved the measurement of acoustic parameters of Thiel embalmed tissue, and the scrutiny on the temperature response of the embalmed liver on FUS heating. Secondly, baseline …
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Experimental Models of Mycobacterial Replication and Killing in Macrophages
… the isolate of MAI did not replicate in freshly explanted monocytes [generation time of 408h]. MAI was more sensitive to oxidative killing than H37Ra, although both mycobacterial species were equally sensitive to H<sub>2</sub>O<sub>2</sub> treatment in cell-free culture media and in sonicated …
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Coordinated Control of Liver-Specific Genes in Cultured Cells from Mice and Rats
… 24 hours when hepatocytes from the liver were explanted and placed into primary culture. Hepatoma cells known to display liver-specific characteristics also transcribed liver-specific genes at low levels similar to primary cultured hepatocytes. In contrast, slices of liver tissue exposed to the …
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Efficacy of Hammerstein models in capturing the dynamics of isometric muscle stimulated at various frequencies
… at fourteen frequencies between 1 to 100Hz. Explanted frog plantaris longus muscle is tested at the nominal isometric length only; hence, the muscle's force-length and force-velocity dependences are neither assumed nor ascertained. The pilot data are fitted using ten different models …
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The clinical utility of tissue polymerase chain reaction, tissue culture, and tissue histology in blood-culture negative infective endocarditis - a South African single hospital experience
… The clinical utility of additional analysis of explanted tissue in a local South African setting has yet to be described. Objectives. This study aims to assess the clinical utility of performing PCR, culture, and histopathology on the tissue of surgically explanted valves in BCNIE patients in a …
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In vitro milk protein secretion by explants of Holstein bull mammary tissue from two different genetic lines
… 15 d before slaughter. Mammary tissue was explanted and cultured for up to 96 h in basal medium (B) which consisted of Media 199 supplemented with fetal calf serum and bovine insulin, or stimulatory medium (St), which was further supplemented with triiodothyronine, estradiol 17-B, …
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Untersuchungen zum Infektpotential von Biomaterialien (Meshes) in der Bauchdecke
… signs of infection. After 7 days the meshes were explanted for histological and microbiological analysis. Calculations of the mesh surface area revealed a higher level for the multifilament mesh. The extent of adherent bacteria corresponded to the estimated filament surface in vitro. In vivo, the …
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Quantifying Renal Swelling during Machine Perfusion using Digital Image Correlation
While machine perfusion of explanted kidneys is theoretically superior to standard cold storage, it may damage potential transplants unless machine-associated swelling is controlled. This thesis presents the effects of perfusate tonicity on renal swelling during hypothermic machine perfusion. …
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Magnetic Micromotors in Assisted Reproductive Technology
… and control, and the oocyte does not need to be explanted for in vitro fertilization. However, if in vitro fertilization was necessary, fertilized oocytes can be transferred back to the fallopian tube by micromotors in a non-invasive manner, to undergo early embryo development in the natural …
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Design, development and validation of a novel mechanical occlusion device for transcervical sterilization
… and invitro deployments in animal tissue and explanted human uteri. The efficacy of the device and the instant occlusion of the fallopian tubes were proved by hydraulic pressure testing of the implanted uteri using saline and methylene blue solution. Initial results suggest that the device …
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Studies of Lung Micromechanics via Hyperpolarized 3He Diffusion NMR
… and expiration in in vivo human subjects and in explanted human and canine lungs. Average alveolar volume, surface area, and the total number of alveoli at each lung volume are calculated from the 3He morphometric parameters. The results suggest that human lungs inflate/deflate primarily by …
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PATHOGENIC MECHANISMS OF TRUNCATED TITIN IN DILATED CARDIOMYOPATHY
… arises, we analyzed a set of 184 explanted posttransplant DCM hearts, 22 of which bore heterozygous titin truncations. To determine if truncated titin was present in these hearts, we used allele specific proteomic mapping of titin, immunoblotting, and a patient specific …
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Modulation of the angiogenic potential in collagen matrices by immobilisation of heparin and loading with vascular endothelial growth factor
… subcutaneous tissue of the rat. Specimens were explanted after varying periods of implantation, the number of capillaries in the CAM and hemoglobin content in the specimens were evaluated: heparinized collagen matrices loaded with VEGF are vascularized to a substantially higher extent as the …
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The use of 3D surface analysis techniques to investigate the wear of matt surface finish femoral stems in total hip replacement
… stems. By examining stems which have been explanted from patients, a method of wear classification has been developed, and also 3D surface measurement techniques have been employed to quantify wear through parametric characterisation and also volume analysis. Initial findings suggested that …
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