Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 92 for “"explant"”.
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A Pilot Study of Primary Culture of Oral Epithelium using the Direct Explant Technique
… to determine the feasibility of using the direct explant technique for tissue that is typically discarded from extracted teeth as a source for mesenchymal stem cells (MSCs). The long-term goal of this research is to identify an easily obtainable source of MSC’s for new and innovative therapies for …
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Poroelastic Finite Element Analysis of a Heterogeneous Articular Cartilage Explant Under Dynamic Compression in ABAQUS
<p> <p>A poroelastic finite element model of a heterogeneous articular cartilage disc was</p> <p>created to examine the tissue response to low amplitude (± 2% strain), low</p> <p>frequency (0.1 Hz) dynamic unconfined compression (UCC). A strong correlation</p> <p>has been made between the relative …
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Investigation of the role of Staphylococcus Aureus toxins in a cartilage explant model of septic arthritis
… arthritis. An in vitro bovine osteochondral explant model of S. aureus-induced septic arthritis was developed in this study. Utilising fluorescence-mode confocal laser scanning microscopy (CLSM), the model, which avoided the complexities of a host immune response, permitted an assessment of …
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An investigation into the molecular mechanisms of hyperbaric oxygen using a human dermal microvascular endothelial cell line and a porcine retinal explant model.
… In this study, HDMEC and porcine retinal explants (a novel explant model) were exposed to treatments simulating HBOT using a bespoke HBO chamber (≥95% oxygen at 2.2 absolute pressures (2.2ATA) for 105 mins), or hyperoxia alone (HYP) (≥95% oxygen) or hyperbaric pressure alone (HYB) (2.2ATA) …
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Genetic engineering for pest resistance in potato (Solanum tuberosum L.)
… response of transformed cells of the target explant used for plant transformation. Twenty four potato cultivars, each represented by three explant sources (leaves, stems, and roots), were screened for their regeneration response on a standard regeneration medium. From this study 'Red Rascal' …
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Studies on the micropropagation of carob : Ceratonia siliqua L.
… field-grown mature trees using axillary buds as explant material. Surface sterilisation and subsequent development of the buds was difficult to achieve due to the production of phenolic exudates by the explants, which were detrimental to plant health. The use of a pre-soak with an antioxidant …
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Extended mechanical stimulations of cartilage for growth and repair
… of articular cartilage in an in vitro model explant system promotes growth and repair. An alternating day mechanical loading protocol consisting of dynamic sinusoidal compression results in long term extracelluar matrix macromolecule biosynthesis and increased biosynthetic rates in the …
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The effect of the growth retardant Paclobutrazol on the in vitro growth and development of Betula and Populus species
… eight to twelve week culture period, after which explants were harvested. At harvest, explants of both species were removed from their culture universals after growing on hard agarose gel media containing a range of PBZ concentrations. Explant fresh weight, height and dry weight was recorded. …
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Regeneration and Transformation of Impatiens walleriana Using Cotyledonary Node Culture
… used technique involves transformation of explant tissue and subsequent regeneration in vitro under aseptic conditions. However, prior to our research there was no regeneration protocol suitable for Agrobacterium-mediated transformation of Impatiens walleriana available. Herein we report …
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The cryopreservation of <i>Picea sitchensis </i>germplasm
… of selected genotypes is undertaken.Three explant types were transferred from the Northern Research station, Roslin to the University of Abertay, Dundee where cryopreservation testing was initiated. Each explant comprised of different anatomical complexities varying from the simplest …
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A collagen-GAG matrix for the growth of intervertebral disc tissue
… into the matrices was assessed by culturing AF explants on top of or between matrices. Cells were able to migrate up to 1 cm from the explant, implying that AF defects of this size could be filled with CG matrices. In the third experiment, explants and explant-matrix constructs were cultured in …
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Effects of Three Corticosteroids on Equine Articular Cocultures In Vitro
… equimolar concentrations on equine articular explant co-cultures in an inflammatory environment. Synovial and osteochondral explant co-cultures from 6 equine cadavers were exposed to IL-1β (10 ng/mL) and various concentrations (10-4, 10-7, or 10-10 M) of MPA, TA, IPA. Concentrations of PGE2, …
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Rapid multiplication of cassava (Manihot esculenta Crantz.) as a potential method to provide disease-free plant materials
When explants were cultured for 3 weeks in a double phase medium, with 0.05 $\mu$M gibberellic acid (GA$\sb3$) in LIMO and AMM solid phase, taller (3 cm), single shoots with $>$3.5 roots per explant were produced, while with APM and GA$\sb3$ cultures were shorter, multiple shoots but without roots. …
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Investigating the integrity of the vasculature and structural relationships of pericytes, astrocytes and the endothelial glycocalyx in an ex vivo hyperglycaemic rat retinal model
… and the retinae were either dissected out (explant) or kept in situ after removal of the anterior segment, lens and vitreous (eyecup). Retinae were superfused continuously (for 1-, 2- or 3 hours) in a chamber with oxygenated Krebs-Henseleit buffer containing either physiological glucose (5.5 …
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Ultrasonography Evaluation of Patency of Implanted Infra-Renal Vascular Grafts in the Rat Model.
… weeks 4, 8 and 12 post operatively. Grafts were explanted if there was any ultrasonographic evidence of occlusion or at twelveweek termination of the study. Explant was preceded by angiography and followed by histological assessment of the grafts for patency. Results: Three of the looped and all …
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An In Vitro mammalian model for investigating locomotor pattern generation
… generating capability of spinal cord-hindlimb explants taken from animals aged from birth to 4 days. The analysis was based on EMG recordings from the gastrocnemius (G) and tibialis anterior (TA) muscles, an antagonist set of muscles used in stepping. In intact animals, electrodes were placed …
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Matrix metalloproteinases in the equine systemic inflammatory response: implications for equine laminitis
… vitro were investigated using a digital laminar explant model. The structural integrity of the explants was tested using an Instron biomechanical testing device, and MMP activity in the explants medium was evaluated using zymography. Doxycycline, oxytetracycline, and flunixin meglumine all …
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Developing cultivated mollusks through establishing primary cell culture methods of Eastern Oyster, Crassostrea virginica, as a model bivalve
… adductor (OAD) cells were obtained via tissue explant, mechanical and enzymatic digestion. The cells were routinely monitored using an inverted microscope for phase-contrast and fluorescence imaging. Culture vessels were coated with surface proteins such as fibronectin, laminin, matrigel, and …
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Understanding the Differential Effects of Dexamethasone on the Metabolism of Healthy and Diseased Articular Cartilage
… proteomic analyses of a bovine ex vivo cartilage explant monoculture model of PTOA progression to identify inflammation-induced catabolic processes associated with disease progression that were attenuated by the addition of Dex. Some of the matrix fragments and inflammatory cytokines appear to be …
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