Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"exopolysaccharide (EPS)"”.
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Rheological, Sensorial and Chemopreventive Properties of Milk Fermented with Exopolysaccharide-Producing Lactic Cultures
… properties of milk fermented with different exopolysaccharide (EPS) producing lactic cultures. Reconstituted (11 % wt/vol) skim milk was fermented with single strains of BPS-producing and non-producing cultures. Fermentation was terminated at pH 4.64 and fermented milk was stored at 4 °C for …
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Production and Characterization of a Novel Extracellular Polysaccharide Produced by Paenibacillus velaei, Sp. Nov
… of producing an unusually large amount of exopolysaccharide (EPS). The EPS contains glucose, mannose, galactose and fucose in a molar ratio of 4:2:1:1. The molecular weight of the EPS is higher than 2x106. The viscosity of 1% EPS is 1300 cP when measured at a shear rate of 1 sec-1. …
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CRISPR3 Regulates Exopolysaccharide Production in Myxococcus xanthus
… spacer of CRISPR3 (3SP13) was found to restore exopolysaccharide (EPS) production to a pilA mutant. Since the deletion of CRISPR3 failed to suppress a pilA mutation and expression of CRISPR3 from a heterologous promoter led to pilA suppression, it was concluded that the 3SP13 transposon …
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Investigating Pulmonary Immune Responses that Detect and Counteract Gram-negative Bacterial Infections
… Gram-negative bacteria produce biofilms through exopolysaccharide (EPS) that provides microbial protection; however, the impact of EPS on detection remains uncertain. Using genome-engineered P. aeruginosa strains, we compared EPS-producers versus non-producers and a virulent E. coli pneumonia …
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Genetic Analysis of the Quorum Sensing Regulator EsaR
… colonize the xylem and overproduce a capsular exopolysaccharide (EPS) at high cell densities. The production of EPS is regulated by an EsaI/EsaR quorum sensing mechanism, homologous to the LuxI/R system. Although activation of the EPS encoding genes by EsaR occurs after it complexes to the AHL …
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Improving Texture and Flavor of Reduced-Fat Cheddar Cheese Using an Exopolysaccharide-Producing Culture and Ultrafiltration
… (Awad et al., 2005a; b; Hassan et al., 2005), an exopolysaccharide-(EPS) producing culture improved textural, melting and viscoelastic properties of reduced-fat Cheddar cheese. However, this EPS-positive cheese developed bitterness after 2 to 3 months of ripening due to increased residual chymosin …
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Demonstration of Interactions Among Dif Proteins and the Identification of Kapb as a Regulator of Exopolysaccharide in Myxococcus Xanthus
… proteins are chemotaxis homologues that regulate exopolysaccharide (EPS) biogenesis. Previous genetic studies suggested that Dif protein might interact with one another as do the chemotaxis proteins in enterics. The interactions among Dif proteins were since investigated with the yeast two-hybrid …
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Novel genes required for proper cell envelope function in the plant symbiont Rhizobium leguminosarum
… mutant had significantly decreased levels of exopolysaccharide (EPS) compared to wild-type, and was also desiccation sensitive and unable to form biofilms. Another mutation mapped to the gene fabF2XL, which is required for synthesis of the unique lipid A very long chain fatty acid (VLCFA). The …
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Genomic diversity of Lactobacillus salivarius
… that produced by CGHbased strain clustering. The exopolysaccharide (EPS) associated genes of L. salivarius strains were found to be highly divergent. The EPS-producing phenotype was found to be carbonsource dependent and inversely related to a strain's ability to produce a biofilm. The genome of …
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Biochemical Characterization of Glycan Assembly Pathway Enzymes
… identifying the first two monosaccharides of the exopolysaccharide (EPS) glycan from Bacillus subtilis, which had not been previously known.
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Understanding the role of TapA in the formation of <i>B. subtilis</i> biofilms: a biochemical and genetic analysis
… B. subtilis biofilm matrix is composed of exopolysaccharide (EPS) and the protein components BslA, TasA and TapA. TasA is a fibre forming protein which has been described in the literature as an amyloid-forming fibre, however, recent work contradicts this finding. The current work focusses …
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Assessing the involvement of BslA in plant-root associated biofilms
… extracellular matrix. The matrix is composed of exopolysaccharides, proteins and nucleic acids, and provides protection to the cells from various stresses. One microorganism that relies on biofilm formation for biocontrol, is the Gram-positive soil-dwelling bacterium Bacillus subtilis. B. …
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Identification and characterization of type III secretion inhibitors in Erwinia amylovora, the causal agent of fire blight of apple and pear
… blight. The type III secretion system (T3SS) and exopolysaccharide (EPS) amylovoran are two major yet separate virulence factors in E. amylovora. Current fire blight management mainly relies on application of copper compounds and antibiotics. However, development of streptomycin resistant E. …
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Role of the Leucine-responsive Regulatory Protein during growth of the bacterial corn pathogen Pantoea stewartii subspecies stewartii in the xylem environment
… Pss grows to high cell density and secretes exopolysaccharide (EPS), forming a biofilm which eventually obstructs water transport, leading to wilting and necrosis. Previous Tn-Seq experiments provided insights into genes that are essential for in planta survival, including the master …
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Characterization of major virulence regulators of Erwinia amylovora
… dollars of economic losses all over the world. Exopolysaccharide (EPS) amylovoran and type III secretion system (T3SS) are two major virulence factors in E. amylovora. However, how these virulence factors are regulated is not completely understood. In bacteria, gene expression is mainly …
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Biofilm formation by Shewanella oneidensis MR-1 towards fundamental understanding of anode respiring communities
… electrode surface and acting as an artificial exopolysaccharide (EPS) biofilm binder. As a demonstration of proof, this methodology was applied to novel biocompatible electrode materials (PHBV-CF) and combined with a laccase catalyzed oxygen reduction cathode in a hybrid biological fuel cell. …
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Posttranscriptional and posttranslational regulation of virulence in erwinia amylovora
… the type III secretion system (T3SS) and the exopolysaccharide (EPS) amylovoran, are strictly required for its pathogenicity. Our previous studies have determined the role of several transcription factors in the regulation of E. amylovora virulence; however, molecular mechanisms of virulence …
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Structure/Function Analysis of the Quorum-sensing Regulator EsaR from the Plant Pathogen Pantoea stewartii
… in the plant xylem and secrete an abundance of exopolysaccharide (EPS). EPS production is regulated by quorum sensing. Two regulatory proteins are key to the process of quorum sensing, the LuxI and LuxR homologues EsaI and EsaR. Most LuxR homologues function as activators of transcription in the …
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Characterization of two prevalent yet distinct conjugation systems among lactococci
… bacteriophage resistance, lactose metabolism and exopolysaccharide (EPS) production. There are practical limitations associated with the isolation of new strains, while there are regulatory hurdles associated with the application of genetically-modified organisms (GMOs). Consequently, the …
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Bioprospecting for novel marine polysaccharides for the life sciences
… as hyaluronic acid (HA), which is the primary exopolysaccharide (EPS) used within medical device formulations to conduct several ophthalmic surgeries, such as cataract removal. Despite this biocompatibility, the use of HA has considerable drawbacks including swelling, pain, injection site …
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