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Showing 1 to 20 of 66 for “"exopolysaccharide"”.
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CRISPR3 Regulates Exopolysaccharide Production in Myxococcus xanthus
… spacer of CRISPR3 (3SP13) was found to restore exopolysaccharide (EPS) production to a pilA mutant. Since the deletion of CRISPR3 failed to suppress a pilA mutation and expression of CRISPR3 from a heterologous promoter led to pilA suppression, it was concluded that the 3SP13 transposon …
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Regulation of Exopolysaccharide Production in Myxococcus Xanthus
… localized type IV pili (Tfp). Besides Tfp, exopolysaccharides (EPS), another cell surface associated component, are also required for M. xanthus S motility. Previous studies demonstrated that the Dif chemotaxis-like signal transduction pathway is central to the regulation of EPS production …
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Investigation of exopolysaccharide production by lactic acid bacteria.
… is an investigation into the production of exopolysaccharides (EPS) produced by strains of Lactobacillus delbrueckii ssp. bulgaricus and Lactococcus lactis ssp. cremoris. These are used in the dairy industry for the production of yoghurt and fermented drinks products. For many years EPS …
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Biochemistry and functional analysis of exopolysaccharide production in Lactobacillus johnsonii
Lactic Acid Bacteria (LAB) produce unique exopolysaccharides (EPS) that are important in food industry but they also play critical role in bacterial interactions during colonisation of the gastrointestinal tract. The role of this layer in the virulence of pathogenic bacteria has been well described …
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The role of rhizobium meliloti exopolysaccharide in nodulation of alfalfa
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1987.
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Functional application of lactic acid bacteria exopolysaccharide in complex food systems
… Weisella cibaria MG1 was capable to produce exopolysaccharides during sucrosesupplemented barley malt derived wort fermentation. Even though the strain dominated the (sucrose-supplemented) wort fermentation, it was found to produce EPS (14.4 g l-1) with lower efficiency than in SucMRS (34.6 g …
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Biosynthesis of Nucleotide Sugar Monomers for Exopolysaccharide Production in Myxococcus Xanthus
… two cellular structures: type IV pili (TFP) and exopolysaccharides (EPS). Previous studies have shown that M. xanthus does not use glucose or any other sugar as a primary carbon source. However, eight monosaccharides, namely glucose, mannose, arabinose, galactose, xylose, rhamnose, …
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Structural Insights into Modification and Export of the Pseudomonas aeruginosa Alginate Exopolysaccharide
… a variety of different components, including exopolysaccharides. During chronic lung infections, P. aeruginosa converts to a mucoid phenotype by constitutive expression of the AlgU/T regulon. As a result, P. aeruginosa overproduces the alginate exopolysaccharide, leading to worse patient …
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Production and bioactivity of Ganoderma lucidum BCCM 31549 exopolysaccharide using submerged liquid fermentation
The RBF strategy has successfully produced fungal mycelial biomass and EPS in a very strictly regulated manner at high productivity rates compared to batch fermentation. The problematic lag phase and seed culture preparation were reduced in length; harvesting volume doubled, yield of product …
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Rheological, Sensorial and Chemopreventive Properties of Milk Fermented with Exopolysaccharide-Producing Lactic Cultures
… properties of milk fermented with different exopolysaccharide (EPS) producing lactic cultures. Reconstituted (11 % wt/vol) skim milk was fermented with single strains of BPS-producing and non-producing cultures. Fermentation was terminated at pH 4.64 and fermented milk was stored at 4 °C for …
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Modification and Translocation of the Biofilm Exopolysaccharide Poly-β(1,6)-N-acetyl-D-glucosamine
… against antibiotics and host defenses. A key exopolysaccharide required for biofilm formation by a number of pathogenic bacteria is poly-beta(1,6)-N-acetyl-D-glucosamine (PNAG). The modification of PNAG through deacetylation and/or hydrolysis by PgaB, BpsB, and IcaB is required for biofilm …
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Physical properties of functional fermented milk produced with exopolysaccharide-producing strains of Streptococcus thermophilus
… on the study of the influence of different exopolysaccharide types produced by two strains of Streptococcus thermophilus on the physical properties of fermented milk. First, the fermentation factors affecting EPS production were studied to ascertain required carbon source and environmental …
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Improving Texture and Flavor of Reduced-Fat Cheddar Cheese Using an Exopolysaccharide-Producing Culture and Ultrafiltration
… (Awad et al., 2005a; b; Hassan et al., 2005), an exopolysaccharide-(EPS) producing culture improved textural, melting and viscoelastic properties of reduced-fat Cheddar cheese. However, this EPS-positive cheese developed bitterness after 2 to 3 months of ripening due to increased residual chymosin …
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Investigating the Roles of the Stk Locus in Development, Motility and Exopolysaccharide Production in Myxococcus Xanthus
Myxococcus xanthus, a Gram-negative bacterium with a developmental cycle, displays a type IV pili (TFP) mediated surface motility known as social (S) gliding. Beside the polarly localized TFP, the fibril or extracellular polysaccharide (EPS) is also required for S-motility to function. It is …
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Impact of pre-acidification, fat replacers and exopolysaccharide producing starter cultures on functionality of low fat mozzarella cheese
This study broadly aimed at developing low fat Mozzarella cheeses containing 6%> fat with improved textural and functional characteristics. The specific aims of this study were to: 1. identify, screen and select strains of EPS producing Streptococcus thermophilus and Lactobacillus delbrueckii …
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Role of the glycanases ExoK and ExsH in regulating the molecular weight of the Rhizobium meliloti exopolysaccharide succinoglycan
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1998.
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Demonstration of Interactions Among Dif Proteins and the Identification of Kapb as a Regulator of Exopolysaccharide in Myxococcus Xanthus
… proteins are chemotaxis homologues that regulate exopolysaccharide (EPS) biogenesis. Previous genetic studies suggested that Dif protein might interact with one another as do the chemotaxis proteins in enterics. The interactions among Dif proteins were since investigated with the yeast two-hybrid …
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The improvement of physical properties of yoghurts by varying casein/whey protein ratio and EPS-producing starter cultures
… in casein to whey protein ratios and types of exopolysaccharide producing starter cultures on physical properties of yoghurts made at 9% and 14% total solids during 28 days of storage at 4°C. There are three main parts of this research: a preliminary study, a comparative study of three methods …
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Characterization of major virulence regulators of Erwinia amylovora
… dollars of economic losses all over the world. Exopolysaccharide (EPS) amylovoran and type III secretion system (T3SS) are two major virulence factors in E. amylovora. However, how these virulence factors are regulated is not completely understood. In bacteria, gene expression is mainly …
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Characterisation of the localisation and function of the Bacillus subtilis YuaB protein during biofilm formation
… synthesised by B. subtilis is composed of the exopolysaccharide, for which the chemical structure is not yet established, and a protein TasA that forms amyloid-like fibres spanning between the cells and anchored to the cells by an accessory protein TapA. A third protein of unknown function, …
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