Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"exogenous DNA"”.
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Investigating the role of apoptosis regulator EndoG on exogenous DNA uptake, stability, replication and recombination
… the cell. It non-specifically cleaves endogenous DNA following apoptosis induction, but is also active in non-apoptotic cells for mitochondrial DNA (mtDNA) replication and may also be important for replication, repair and recombination of genomic DNA. The aim of our study was to examine whether …
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Effect of Liposome Encapsulation of Cauliflower Mosaic Virus and Its Dna on Plant Infectivity and Culture of Turnip (Brassica Rapa L.) Protoplasts, Their Uptake and Expression of Exogenous Dna
… of cauliflower mosaic virus (CaMV) and its DNA. When grown at temperatures below 24 C, inoculated half-leaves of turnip (Brassica rapa L. cv. Just Right) produced distinct lesions. Decolorizing and staining leaves for starch increased 2 to 3 times the number of observable lesions. This …
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An examination of the role of IFI16 in detecting viral DNA in human immortalised keratinocytes
The presence of exogenous DNA in the cytosol results in the activation of the DNA sensor cyclic GMP-AMP synthase (cGAS). cGAS produces the second messenger cyclic GMP-AMP (cGAMP) which binds to and activates the endoplasmic reticulum adapter STimulator of INterferon Genes (STING). Activated STING …
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Nuclear Transformation of Chlamydomonas Reinhardtii to Allow for Studies on Circadian Expression of the β-Tubulin Gene
… reinhardtii was agitated in the presence of exogenous DNA, cell wall-digesting autolysin, glass beads and polyethylene glycol. Autolysin had been harvested successfully during mating of two different mating type strains, the optimal antibiotic concentration in the selection medium had been …
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Mechanism of Permissive Cleavage Activity of TevCas12a
… breaks leaving non-compatible ends for exogenous DNA insertion and specific deletions. TevCas12a was expected to make two double-strand breaks, one by I-TevI and the other from Cas12a. However, when tested in vitro, TevCas12a cut the DNA substrate multiple times, rapidly degrading the …
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Effects of conjugated oligoelectrolytes on cell transformation
… increase their competence in accepting exogenous DNA. This study tested the effect of the COE DSSN+ combined with electroporation on the transformation of Escherichia coli cells. Multiple experiments with varying concentrations of DSSN+ and varying voltages provided bacterial colony …
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Synthetic biology approaches for engineering diverse bacterial species
… subtilis, ICEBsJ, was engineered to deliver exogenous DNA to diverse strains of undomesticated Gram-positive bacteria. Engineered ICEBsI conjugation was demonstrated in twenty different bacterial strains, spanning sixteen species and five genera. To demonstrate ICE's utility in creating new …
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Using CRISPR/Cas9 to modify the genome of cattle
… using sperm-mediated gene transfer (SMGT). Naked DNA binds naturally to sperm, and can be used to produce transgenic offspring in pigs and cattle. In this experiment, we analyzed methods to select thawed bovine sperm, and evaluated the binding of exogenous DNA to those sperm. Liposome preparation …
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High resolution alignment of the physical and genetic maps of the dpy-14 region of chromosome I in Caenorhabditis elegans
… with cosmid clones containing C. elegans genomic DNA was used to generate stable transformants that carried heritable extrachromosomal arrays composed of exogenous DNA. The usefulness of these arrays as genetic mapping tools was explored in this study. Germ line stability of heritable arrays was …
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Genetic and Cytological Analysis of the Spindle Assembly Checkpoint Component BUB-3 in DNA Damage Response
The spindle assembly checkpoint and the DNA damage response have been studied for decades as independent pathways exerting separate functions. However, crosstalk between these cellular processes has emerged. The extent of overlap between these pathways is still unknown, especially in multicellular …
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Functional Characterization of the Streptococcus sanguinis com Regulon
… genetic competence, the ability to acquire exogenous DNA into its genome. In the well characterized system of S. pneumoniae, genetic competence requires a quorum sensing system encoded by the early comCDE genes, as well as late genes controlled by the alternate sigma factor, ComX. Previous …
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New Insights into the Roles of Human DNA Damage Checkpoint Protein ATR in the Regulation of Nucleotide Excision Repair and DNA Damage-Induced Cell Death
… is frequently threatened by endogenous and exogenous DNA damaging reagents that may lead to genome instability and cancer. Cells have evolved multiple mechanisms to repair DNA damage or to eliminate the damaged cells beyond repair and to prevent diverse diseases. Among these are ataxia …
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ELECTROPHORETIC AND ENZYMATIC IDENTIFICATION OF PROTEINS ASSOCIATED WITH THE DEVELOPMENT OF COMPETENCE IN BACILLUS SUBTILIS
… by Mn('2+) and are most active on denatured DNA. The fourth (monomeric MW; 17,000) is also Mn('2+)-activated but is distinguished by its preference for native DNA. The nuclease activities which are reduced in competent cells are all primarily Mn('2+)-activated. Three of these nucleases …
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Understanding cell fate decisions in response to 0⁶-Methylguanine DNA lesions
… is constantly challenged by both endogenous and exogenous DNA damaging agents. DNA damage, if left unrepaired, can give rise to permanent genetic alterations that ultimately increase our risk of cancer and other diseases. To combat these threats, eukaryotic cells activate a DNA damage response …
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Mosquito Transposable Elements and piwi Genes
… new strategy due to their ability to integrate exogenous DNA into chromosomes. They could potentially be useful tools in assisting the spread of disease-resistant genes in mosquito populations. This research focuses on two related subjects, TEs and their regulation. The first subject is on a …
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Understanding The Molecular Mechanism Of Manganese Oxidation In Leptothrix Discophora Ss1
… and a procedure for transformation with exogenous DNA via conjugation was developed and optimized, resulting in a maximum transfer frequency of 5.2*10-1 (transconjugant/donor). Genetic manipulation of Leptothrix was demonstrated by disrupting pyrF via chromosomal integration of a plasmid …
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The Role of Adaptor Proteins in rDNA Double-Strand Break Repair by Homologous Recombination
… threatened by a number of endogenous as well as exogenous DNA damaging agents. Among the various lesions, DNA double strand breaks (DSBs) represent a particularly deleterious threat for a cell. In order to maintain genome stability and integrity, cells have evolved a highly organized network of …
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Inflammasome upregulation and activation in photoreceptors of type 2 diabetes and N-methyl-N-nitrosourea models is attenuated by Wedelolactone
… and porosity, leading to the extravasation of mtDNA. In such scenarios, AIM2 inflammasome signalling is activated in response to DNA-sensing, leading to inflammation and cell death. Wedelolactone has been previously identified as a novel CASP4/11 inhibitor and its potential to modulate the …