Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"ex vivo culture"”.
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Optimizing Ex Vivo Culture of Satellite Cells
… pathways. I showed the effect of in vitro culture on transcription of various myogenic regulatory factors. I also showed the effect of various inhibitors on these factors. I found that treatment of freshly isolated satellite cells and of established myoblast cells with SR1 (Stemregenin 1, …
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Ex vivo culture of patient tissue and examination of gene delivery
… tissue, cut into thin slices and maintained ex vivo and is universally applicable to gene delivery methods, using a real-time luminescence detection method to assess gene delivery. The nature of the ex vivo culture system permitted examination of specific physiological variables, the …
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Factors affecting neurogenesis after application of human mesenchymal stem cells in ischemic rat model
… reported the results of clinical trial of ex vivo culture expanded autologous mesenchymal stem cells (MSCs) in patients with severe ischemic stroke. We proposed that an optimal condition, for either donor or recipient, may exist and enhance the effects of the mesenchymal stem cells in those …
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Quantitative correlations among human mesenchymal stem cell mechanical properties and biological function
… from infant or adult sources are heterogeneous, exhibit poor overall integration in host tissues, and their differentiation and proliferation capacities are limited by ex vivo culture. On the other hand, fetal MSCs (fMSCs) derived from fetuses are more homogeneous, plastic and grow faster than …
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Targeting Glutamine Metabolism in Kidney Development and Polycystic Kidney Disease
… of cyst formation. We utilized in vitro cell culture coupled with ex vivo culture of embryonic kidneys with defined media allowed us to determine which metabolic pathways were affected by the deficit of Lkb1. Our results revealed that Lkb1 mutant cells and embryonic kidneys require glutamine …
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SIGNALLING TISSUE RENEWAL AND CRYPT SURVIVAL IN THE HUMAN COLONIC EPITHELIUM AND BARRETT’S OESOPHAGUS
… crypts were isolated and placed into 3D tissue culture conditions optimised for steady-state tissue renewal. The role of Wnt and TGFβ/BMP signalling pathways in tissue renewal was investigated. Native human colonic crypts exhibited distinct activation profiles for canonical Wnt, TGFβ and BMP …
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The Impact of Clinically Relevant Culture on Human Haematopoietic Stem Cells: A Kinetic Analysis at Single Cell Resolution
Haematopoietic stem cell (HSC) ex vivo gene therapy is now successfully used to treat an in-creasing number of monogenic disorders affecting the blood system. The ultimate efficacy of this therapy depends upon successful targeting of long-term haematopoietic stem cells (LT-HSCs), which are …
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Transcription factor modulation in T-cells to improve cancer immunotherapy
Adoptive transfer of T-cells expressing chimeric antigen receptors (CAR-T) effectively treats refractory hematologic malignancies in a subset of patients, but can be limited by the inability of ex vivo culture systems to expand T-cells sufficiently for effective dosing while producing highly active …
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Impaired ketogenesis is implicated in intestinal stem cell function and epithelial regeneration in ulcerative colitis
… regenerate the entire epithelium. ISCs therefore exist in a highly active state and resultantly are vulnerable to changes in energy dynamics in the intestine. Mitochondria, which amongst other functions are critical for energy production are therefore crucial for maintenance of the stem cell niche …
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Characterization of Genome-Wide Binding of NUCLEAR-FACTOR I-X in Hematopoietic Progenitor Cells
<p>We report that ectopic expression of Nfix in primary mouse HSPC extended their ex vivo culture from 20 to 40 days. HSPC overexpressing Nfix displayed hypersensitivity to supportive cytokines and reduced apoptosis when subjected to cytokine deprivation compared to controls. Ectopic Nfix resulted …
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Analysis of Therapeutic Effect after Human Mesenchymal Stem Cell Transplantation in Ischemic Stroke Rat
… in a rat stroke model (Part 3). Part I: Although ex vivo culture-expansion is necessary to use autologous MSCs in treating stroke patients and several researchers have utilized culture-expanded cells in their studies, the effects of culture-expansion on neurogenesis and trophic support are …
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Novel approaches to assess cellular interactions and their role in the pathology and treatment of lymphoproliferative disorders
… lymphoid tissues, where they engage in the complex cellular and molecular interactions that underlie their survival, proliferation, and drug resistance. Further emphasising this importance, has been the observation that highly effective small molecule inhibitors that target key components of the …
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Intestinal macrophages in health and inflammation
… ability to phagocytose particulate material and exert bactericidal activity, they are unable to produce pro-inflammatory mediators. Thus it has been suggested that intestinal mφ are functionally adapted to the microbe-rich, immunostimulatory environment of the gut, where strong inflammatory …
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Elucidation of mammary gland development: combination of novel techniques to study proliferation and stem cells
… to mammary stem cells (MaSCs). However, despite extensive efforts over the past 60 years, definitive characterisation of the stem and progenitor cells of the mammary gland has yet to be achieved. A number of recent conflicting, lineage tracing studies have served only to fuel the fires of …