Global ETD Search
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Showing 1 to 17 of 17 for “"eukaryotic transcription"”.
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On the Diversity of Eukaryotic Transcription Factor Specificities
Transcription factor (TF) DNA-binding specificities (motifs) are often conserved between species, and many TFs have conserved functions over long evolutionary distances. As a consequence, changes in gene expression are largely attributed to evolution of cis-regulatory DNA sequences rather than …
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Transcription regulation in Plasmodium falciparum : functional characterisation of general transcription factor IIB
… In eukaryotes, regulation of RNA Polymerase II transcription initiation is one of the main mechanism of gene expression control. Past research has revealed the presence of crucial elements of RNA Polymerase II (RNAPII) transcription machinery in P. falciparum, however, the precise transcription …
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Isolation and Characterization of the Chloroperoxidase Gene From Caldariomyces Fumago
… AATAAA polyadenylation signal. The start site of transcription was mapped by primer extension analysis and found conform to the general pattern of eukaryotic transcription initiation sites. A search of a portion of the BIONET databases for sequences similar to that of the cpo gene did not find any …
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Med12 Is A Critical Regulator of Neural Crest Lineage and Nervous System Myelination
… a multi-subunit protein complex integral to the eukaryotic transcription machinery. MED12 is a Cdk8- regulatory kinase module subunit directly implicated in human disease and is genetically altered in neurological disease and cancer. Numerous attempts at generating an<em> in vivo</em> system to …
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The Role of Phosphorylation in Modulating PC4 Function as a General Cofactor
… polymerase II, which play an essential role in eukaryotic transcription, we have developed an effective purification scheme that allows TFIIH and RNA polymerase II to be purified from HeLa-derived clonal cell lines that conditionally express the FLAG-tagged p62 subunit of human TFIIH and the …
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Mediator and cell cycle regulation
… is an evolutionarily conserved coregulator of eukaryotic transcription. Mediator functions as a bridge between gene-specific transcription factors and the RNA polymerase II transcription machinery. Mutations affecting Mediator function have been associated with a large number of diseases from …
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Protein-DNA Recognition Models for the Homeodomain and C2H2 Zinc Finger Transcription Factor Families
Transcription factors: TFs) play a central role in the gene regulatory network of each cell. They can stimulate or inhibit transcription of their target genes by binding to short, degenerate DNA sequence motifs. The goal of this research is to build improved models of TF binding site recognition. …
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DNA Recognition by Helix-Loop-Helix Proteins
<p>The Helix-Loop-Helix (HLH) family of eukaryotic transcription factors comprises a large number of proteins which play key roles in homeostasis, the regulation cell proliferation, and differentiation. These proteins share a phylogenetically conserved bipartite b/HLH domain responsible for …
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Characterizing cell-cycle as a global regulator of stochastic transcription and noisy gene expression in S. cerevisiae
… to the inherently stochastic nature of transcription. Expression statistics from multiple organisms are consistent with an influential model of "bursty" expression, where promoters are generally inactive but infrequently produce multiple mRNA. But fluctuations in the cell environment can …
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Determining protein interaction specificity of native and designed bZIP family transcription factors
… their interactions. The basic leucine-zipper transcription factors (bZIPs) are a class of eukaryotic transcription factors that form either homodimers or heterodimers that bind to DNA in a site-specific manner. bZIPs are similar in sequence and structure, yet bZIP protein-protein interactions …
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Investigating the role of intra- and extra-cellular modulators of the transcription factor NF-kappaB
The nuclear factor-kappa B (NF-kappaB) family of transcription factors play a pivotal role in the inflammatory and immune responses, as well as cell growth and survival. Since its discovery 25 years ago, NF-kappaB has emerged as one of the most intensely studied eukaryotic transcription factors, …
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Functional divergence of the RNA polymerase II transcription machinery in Plasmodium falciparum
… of three Plasmodium falciparum general transcription factors - two TBP family proteins designated the TATA-binding protein (TBP) and TBP like protein (TLP), and the P. falciparum orthologue of general transcription factor IIB (TFIIB). Through the biochemical characterisation of …
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Biomolecular condensation of intrinsically disordered regions in P. falciparum and H. sapiens transcription factors
… expression programme, and its highly divergent transcriptional machinery suggests unique mechanisms of transcriptional control. Biomolecular condensation, driven by intrinsically disordered regions (IDRs) in proteins, has emerged as a previously underappreciated mechanism of cellular …
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Mutational analysis of the human histone chaperone, Nap1, in nucleosome disassembly at the HTLV-1 promoter
… within the DNA sequence for processes such as transcription and replication. One of the mechanisms by which the genetic information can be accessed is post-translational modifications of the histone tails. Post-translational modifications, such as acetylation, act to neutralize charges on the …
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Overactive NF-KB signaling as a druggable target and evaluation of parthenolide an NF-KB inhibitor in canine cancer
… proteins are a family of structurally related, eukaryotic transcription factors that have 400+ genetic targets, and are involved in many vital cellular processes, including innate immunity, inflammatory responses, development, cellular growth, and survival. Not surprisingly, overactivation of …
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Control of uteroglobin gene expression
Eukaryotic nuclear genes are regulated by a complex array of transcription factors that influence RNA polymerase II activity. Included among these factors are the cellular receptors for steroid hormones. The rabbit uteroglobin gene expression is positively stimulated by progesterone, and binding …