Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"eukaryotic gene expression"”.
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REGULATION OF EUKARYOTIC GENE EXPRESSION BY mRNA CAP BINDING COMPLEX AND CAPPING MACHINERY
A characteristic feature of gene expression in eukaryotes is the addition of a 5' terminal 7-methylguanine "cap" to nascent pre-messenger RNA (mRNA) in the nucleus. It is the 5'capping process, which proves vital to creating a mature mRNA. The synthesis of an mRNA followed by its capping is a …
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Quantitative isoforrm profiling & isoform convergence
… pre-messenger RNA splicing is a crucial step in eukaryotic gene expression, and therefore it is subject to tight regulation. Given its importance in conferring protein diversity, alternative splicing is sensitive to changes in cellular states including malignancy. We present a new paradigm by …
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IDENTIFICATION OF SPLICING PATHWAY MUTATIONS VIA TARGETED SEQUENCING
… is an essential and regulated component of eukaryotic gene expression. While ever-increasing numbers of human genetic diseases can be linked to defects in the splicing pathway, our molecular understanding of how these mutations disrupt this complex process remains incomplete. To identify …
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Quantitative modeling of 5' splice site subclass regulation and evolution
… is an essential molecular process required for eukaryotic gene expression. In this thesis, I present a previously unknown mechanism of splicing regulation where a family of splicing factors, the LUC7 family, compete to differentially impact 5→ splice site (5→ SS) selection in a …
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Cell cycle-dependent regulation of the human RNA cap methyltransferase (RNMT)
… proliferation as it influences several steps in eukaryotic gene expression including transcription, pre-mRNA processing, RNA export and translation. The N-7 methylguanosine cap is added co-transcriptionally to RNA pol II transcripts. In mammals, two enzymes catalyse the synthesis of the …
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Structural characterization of a protein/RNA complex : human TAP/NXF1 protein/retroviral CTE RNA
Abstract Eukaryotic gene expression involves several steps, including transcription, post-transcriptional processing of pre-mRNA transcripts and export of the correctly processed mRNAs from the nucleus to the cytoplasm, where translation takes place. Evidence has accumulated suggesting that these …
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Dicer deletion and short RNA expression analysis in mouse embryonic stem cells
RNA interference (RNAi) manages many aspects of eukaryotic gene expression through sequence-specific interactions with RNA. Short RNAs, 20-30 nucleotides in length, guide the various effector proteins of RNAi to silence fully or partially complementary targets. The sequencing of endogenously …
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Cloning of a putative human oncogenic virus, BK
… the polyoma viruses. The papilloma viruses are generally larger than the polyoma-viruses, having a genome of approximately 5 x 106 daltons compared with 3,3 x 106 daltons, and virions of approximately 55nm diameter as opposed to 41nm. The papilloma viruses generally produce benign epithelial …
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Long-Term Quantitative Microscopy: From Microbial Population Dynamics to Growth of Plant Roots
… processes, including bacterial chemotaxis, eukaryotic gene expression and y development. Extending measurements to long time scales allows complete observation of processes that are otherwise studied piecemeal, such as development and evolution. This thesis describes the development of two …
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Sequence-Dependent & -Independent Effects of Intron-Mediated Enhancement (IME)
Introns are ubiquitous features of eukaryotic genes, and their precise removal from pre-mRNA transcripts by the spliceosome is an essential step in gene expression. Genomic deletion of an intron from a gene tends to reduce its expression, and addition of an intron tends to increase it. This …
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Functional Analysis of Drosophila Integrator Complex In Snrna 3' End Processing
… nuclear RNAs (U snRNAs) play essential roles in eukaryotic gene expression by facilitating the removal of introns from mRNA precursors and the processing of the replication-dependent histone pre-mRNAs. Formation of the 3’ end of these snRNAs is carried out by a poorly characterized, …
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Development of in vitro iCLIP techniques to study spliceosome remodelling by RNA helicases
… RNA) splicing is a fundamental process in eukaryotic gene expression. In order to catalyse the excision of the intervening intronic sequence between two exons, the spliceosome is assembled stepwise on the pre-mRNA substrate. This ribonucleoprotein machine is extremely dynamic: both its …
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Computational Analysis of Transcriptional Regulation
… his isolation of ’nuclein’ in 1869 would prove. Eukaryotic gene expression is a noisy process that is subject to multiple layers of regulation. Key features of this are the three-dimensional (3D) chromatin organisation of eukaryotic genomes and the post-transcriptional control of RNA fates. …
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Interpretable Computational Modeling of pre-mRNA Splicing for Multiple Eukaryotic Species
One of the key steps in eukaryotic gene expression is pre-mRNA splicing, whereby intronic sequences are excised from immature pre-mRNA transcripts and the remaining exonic sequences are joined together. This process is catalyzed by the spliceosome, a large complex of proteins and RNAs. A variety of …
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The Domains of The Catalytic Subunit of The Eukaryotic Rna Degrading Exosome, Rrp44P, Have Distinct Functions
… In the cytoplasm, the exosome degrades mRNA in a general mRNA turnover pathway and in several mRNA surveillance pathways. In the nucleus, the exosome processes RNA precursors to form small, stable, mature RNA species, including rRNA, snRNA, and snoRNA. In addition to processing these RNAs, the …
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Comparative Analysis of Alternative Splicing in Homo sapiens, Mus musculus and Rattus norvegicus Transcriptomes
… meaningful insight into functional properties of genes and complexity of genomes. Alternative splicing is one of the major mechanisms contributing to the complexity of genomes. This important cellular process generates several different messenger RNA transcripts from a single gene, expression of …
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Optimizing nuclear transgene expression and genome editing for high-value compound production in the green microalga Chlamydomonas reinhardtii
… amounts of toxic waste. Various organisms were genetically engineered to express various terpene synthases, however, photosynthetic model organisms like the green alga Chlamydomonas reinhardtii only recently gained interest as sustainable production chassis. This eukaryotic microalga inherits …
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REGULATION OF EUKARYOTIC TRANSCRIPTIONAL ELONGATION AND ASSOCIATED DNA REPAIR
Transcriptional elongation is a crucial step in eukaryotic gene regulation whose mis-regulation leads to cellular pathologies. This makes it quite imperative to aim for a better understanding of the processes regulating transcriptional elongation. An important process promoting the association of …