Global ETD Search
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Showing 1 to 20 of 41 for “"eukaryotic Initiation Factor"”.
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Characterisation of the eukaryotic initiation factor 2alpha kinases of Plasmodium falciparum
… response is mediated through phosphorylation of eukaryotic initiation factor 2alpha (eIF2alpha), which results in selective translation of mRNAs encoding stress response proteins. Post-transcriptional control of gene expression is suspected to play an important role in P. falciparum. Importantly, …
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The role of eukaryotic initiation factor 4E (eIF4E) in osteosarcoma metastasis
… of the mRNA cap-binding phosphoprotein, eukaryotic initiation factor 4E (eIF4E) is considered to be both rate and process limiting. We describe for the first time, the biological role of eIF4E (new biology) in metastatic osteosarcoma. We employed a comparative approach to study the …
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Eukaryotic Initiation Factor 5A2 (EIF5A2) Contributes to Ovarian Tumor Growth and Metastasis
<p>Ovarian cancer has the highest mortality rate among all gynecological malignancies due to lack of effective biomarkers for early diagnosis. The majority of ovarian cancer patients are already at an advanced stage when diagnosed. In addition, ovarian cancers often become chemoresistant and …
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Screens for non-eukaryotic initiation factor two alpha dependent activation of ATF4 translation
… has direct consequences on cellular fitness. Eukaryotic cells have developed many different pathways to signal nutrient availability, cellular stress, and general environmental changes in order to maintain homeostasis, accumulate biomass, and divide. Less is known about how these signaling …
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Elucidating the role of eukaryotic initiation factor 5B (eIF5B) in non-canonical translation initiation
… upregulated through uORF- mediated translation initiation. eIF5B has been shown to deliver initiator-tRNA during non-canonical translation initiation. As such, we defined the role of eIF5B in the non-canonical translation of ATF4, and p27. Through polysome profiling and luciferase reporter …
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Role of Ribavirin, an Anti-Viral Agent, In Eukaryotic Initiation Factor 4e (Eif4e) Subcellular Localization and Function
Expression of eukaryotic initiation factor 4E (eIF4E) is necessary for active cellular growth and catalysis of the rate-limiting step in cap-dependent protein synthesis3-5. Studies in the past decade have shown that eIF4E contributes to malignancy by selectively facilitating the translation of a …
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The role of eukaryotic initiation factor 5A and its cellular localization in developing Zea mays plant tissue.
Eukaryotic initiation factor 5A is an 18 kDa protein that has been implicated in a wide variety of cellular activities, but its primary role, especially its role in translation, has been a mystery. Investigation of its localization in developing maize endosperm and root and leaf tissue have …
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Point mutational examination of eukaryotic initiation factor 4E (eIF4E), inclusion bodies analysis, and Barley Yellow Dwarf Virus (BYDV) exonuclease resistant RNA structure mapping and prediction
<p>Eukaryotic initiation factor 4E (eIF4E) binds the 5’m7GTP cap structure of mRNA in order to facilitate effective translation. Recessive eIF4E alleles harboring naturally occurring point mutations have been associated with resistance to viral infection, particularly in members of the genus …
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Eukaryotic Initiation Factor 3 Interactions with Structural Elements of Barley Yellow Dwarf Virus Untranslated Regions Reveal Details of a New Cap-Independent Translation Initiation Model
… 5′ and 3′ untranslated regions (UTRs) to recruit eukaryotic initiation factors (eIFs) and ribosomes. BYDV’s 3′ translation enhancer (BTE) is a cruciform structure capable of recruiting the cap-binding complex eIF4F, the large scaffolding complex eIF3, and the 40S ribosomal subunit. Together eIF3, …
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Elucidating the Roles of Eukaryotic Initiation Factors Involved in DAP5 Mediated Translation
<p>Translation initiation in eukaryotes is a highly regulated process essential for accurate protein synthesis. It is a dynamic process that involves a complex interplay between messenger RNAs (mRNAs), ribosomal subunits, and a host of initiation factors, ensuring precise start codon selection and …
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Identifying a role for nonessential elF3 subunits eif-3.K and eif-3.L in the regulation of endoplasmic reticulum homeostasis and longevity in Caenorhabditis elegans
The eukaryotic initiation factor 3 (elF3) is a protein complex composed of 13 subunits in mammals, and is an essential scaffold of the molecular interactions required for the formation of the 43S preinitiation complex (PIC). While these 13 subunits are broadly conserved within the eukaryotic …
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Role of 4E-BP2 in neuronal translation
… learning and memory. 4E-BP2 (Eif4ebp2, Eukaryotic Initiation Factor 4E-Binding Protein 2) is the predominantly expressed 4E-BP in the mammalian brain and represses cap-dependent translation initiation, by binding to eIF4E (Eif4e, eukaryotic Initiation Factor 4E). As a master regulator of …
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Mechanisms Underlying The Heterogeneous Sensitivities of Cancer Cells to Proteasome Inhibitors
… protein synthesis by phosphorylation of the eukaryotic initiation factor 2-α subunit (eIF2α). In a panel of 10 distinct human pancreatic cancer cells, we showed marked heterogeneity in the ability of cancer cells to induce eIF2α phosphorylation upon stress (eIF2α-P); lack of inducible eIF2α-P …
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Determinants of translational efficiency in Saccharomyces cerevisiae
… 3, I discuss the regulation of translation initiation to introduce Chapter 4, in which I explore how the RNA binding specificity of the core translation factor, yeast eukaryotic initiation factor 4G (eIF4G), contributes to genome-wide competition between mRNAs. Finally, I will discuss future …
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Understanding the Role of Hypusine Biosynthesis in Endocrine-Exocrine Crosstalk
… Our lab has shown that the mRNA translation factor eukaryotic initiation factor 5A (eIF5A), when in its post-translationally modified “hypusinated” form, plays a role in pancreas development. The hypusination of eIF5A requires the rate-limiting enzyme deoxyhypusine synthase (Dhps) to post- …
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The effect of alternative splicing on key regulators of the integrated stress response
… phosphorylates serine-51 of the α subunit of eukaryotic initiation factor-2 (eIF2), a translation factor that delivers initiator tRNA to ribosomes. Phosphorylation of eIF2α inhibits general translation, which conserves energy and nutrients and facilitates reprogramming of gene expression for …
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Genetic Mechanisms Responsible for Dietary Restriction Dependent Lifespan Extension in Drosophila Melanogaster: A Role for Muscle Tissue
… specifically the translational repressor, eukaryotic initiation factor eIF4E binding protein (4E-BP). Overexpression of constitutively active d4E-BP in the muscle tissue of Drosophila melanogaster led to starvation resistance and increased activity in flies fed a nutrient rich diet. …
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Iron regulates mRNA translation initiation through RNA iron responsive element (IRE)
… concentration elevation. This research shows 1. Eukaryotic initiation factor 4F (eIF4F) binds to IRE-RNA with high affinity and works as a positive control element in mRNA translation. 2. eIF4F, IRP competitively bind to IRE-RNA. 3. Fe<sup>2+</sup> increases eIF4F/IRE-RNA binding affinity, which …
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The Interaction Between Eukaryotic Translation Initiation Factor eIF4G and 3’ Cap Independent Translation Element of Barley Yellow Dwarf Virus Is Affected by Multiple Initiation Factors
… BTE mediated translation primarily depends on eukaryotic initiation factor eIF4G. BTE binds to eIF4G; however, the details of BTE initiated translation are still unclear. Three eIF4G deletion mutants with different domain organization were used to investigate BTE interaction with eIF4G: …
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