Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 82 for “"eukaryote"”.
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Cryopreservation of eukaryote algae
In an attempt to expand the knowledge of the diversity of algal taxa that are capable of successful recovery after freezing to and thawing from -196°C, 365 algal strains representing 13 algal classes were studied using various cryoprotectants at several concentrations. A cryopreservation protocol …
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ELLIE - Exteroceptive Light Locomotion in Eukaryote-Fungi
<p> Over the past decade, fungal research and its technological applications have expanded across multiple disciplines, including the emerging field of biohybrid systems. This thesis develops and evaluates a wireless, untethered mobile robot controlled by the action potential-like activity …
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Long-Term Evolution of the Complex Eukaryote Tetrahymena thermophila
Evolutionary biologists have long sought to understand what factors affect the rate and repeatability of adaptive outcomes. To better understand the role of temperature in determining adaptive trajectories, we evolved populations of different genotypes, including populations that just had sex and …
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Trends in Eukaryote Body Size in an Ecological and Evolutionary Context
… of evolution. This volume examines body size of eukaryotes and its correlates in various temporal and spatial contexts in three distinct studies. The first study investigates the relationship between parasitism and body size of modern bivalve hosts. Individuals of Protothaca staminea were …
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Evolution of Transcription in Archaea and the Early-Diverging Eukaryote, Giardia Lamblia
… transcription components in an early-diverging eukaryote. It is apparent that the evolution of transcriptional machinery can be subdivided into the evolution of a core system and the evolution of a peripheral set of factors modulating the activity and specificity of the core. We see the core in …
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Functions of the eukaryote-specific ribosomal protein Asc1 /RACK1 in gene-specific translational activity
… a focus on the RACK1 protein (Asc1 in yeast), a eukaryote-specific ribosomal protein with many proposed functions in both cellular signaling and translation. In Chapter 2, I will present evidence that the Asc1 protein is required for efficient translation of a specific set of mRNAs with short …
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Evidence for continuous potential for gene transcription during the cell cycle of a eukaryote
… for gene expression is continuous in this eukaryote. A time lag was observed between the beginning of DNA replication and the initial rise in potential for isocitrate lyase gene expression. The control of gene expression in Chlorella appeared to be similar to that found in a fission yeast.
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The evolutionary impacts of secondary structures within genomes of eukaryote-infecting single-stranded DNA viruses
… splicing, and replication, within the genomes of eukaryote-infecting ssDNA viruses (Circoviridae, Anelloviridae, Parvoviridae, Nanoviridae, and Geminiviridae). I show that purifying selection tends to be stronger at base-paired sites than it is at unpaired sites and, wherever mutations are …
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Induktion und Reparatur von DNA-Doppelstrangbrüchen nach kombinierter Einwirkung von Cisplatin und Bestrahlung auf eukaryote Zellen
Am Modell der Hefe Saccharomyces cerevisiae als eukaryote Zelle wird anhand der Induktion und Reparatur von DNA-Doppelstrangbrüchen die Wirksamkeit einer kombinierten Radio- und Chemotherapie gezeigt. DNA-Doppelstrangbrüche können durch ionisierende Strahlung und sekundär durch zelluläre …
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Host-Microbe Relations: A Phylogenomics-Driven Bioinformatic Approach to the Characterization of Microbial DNA from Heterogeneous Sequence Data
… come as no surprise that when the genome of a eukaryote is sequenced, a medley of bacterial sequences are produced as well. These sequences can be highly informative about the interactions between the eukaryote and its bacterial cohorts; unfortunately, they often comprise a vanishingly small …
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Isolation and molecular genetic analysis of conditionally lethal yeast mutants in glycosylphosphatidylinositol anchoring
… of GPI anchoring deficiencies in the unicellular eukaryote Saccharomyces cerevisiae.
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A Bioinformatics Approach to Identifying Radical SAM (S-Adenosyl-L-Methionine) Enzymes
… were more apparent in prokaryotes than in eukaryotes. Although eukaryotes tend to have fewer radical SAM enzymes than prokaryotes, we were able to analyze uncharacterized radical SAM enzymes from both an aerobic eukaryote (Entamoeba histolytica) and a eukaryote capable of oxygenic …
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Analysis Of Yscf And Associated Homologs And The Presence Of An Inflammatory Molecular Pattern
… a conduit of effectors from bacterium to eukaryote and is exposed to the host immune system. This thesis explores YscF and its associated needle homologs and the possibility of recognition as pathogen-associated molecular patterns (PAMP) by macrophage tissue culture. Interestingly, …
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Eukaryotic Microbes in the Deep Sea: Abundance, Diversity, and the Effect of Pressure
… Here, I present three studies on microbial eukaryotes in the deep sea: first, a study of the abundance of microbial eukaryotes in the deep sea, second, a quantitative approach to study broad-scale diversity in the deep sea; and last, a series of experiments to explore the effect of deep-sea …
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Charakterisierung der zellulären Bindung und Aufnahme von clostridialen Toxinen
… exprimiert, um die Bindung und Translokation an eukaryote Zellen zu untersuchen. Es konnte gezeigt werden, dass ein 1820 Aminosäuren umfassender C-terminaler Bereich des Toxins, das Fragment CDB547-2366 für die zelluläre Bindung und Translokation verantwortlich ist. Dieses Fragment wurde in …
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Molecular and Genetic Characterization of SAS Genes in Position Effect Silencing in Saccharomyces Cerevisiae
… of multiple pathways of positon effects in any eukaryote.
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Contributions to the Proterozoic and Cambrian Evolution of Eukaryotes
… of Proterozoic-Cambrian evolution of eukaryote life. Chapter 1 provides, for the first time, a quantitative characterization of the evolutionary trends of Proterozoic macroalgae. The analysis reveals that morphological disparity of Paleoproterozoic macroalgae was low but increased in …
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Investigating a novel Asgard archaeon and its tubulin-like proteins
… Archaea has challenged the definition of a eukaryote, suggesting that certain ‘eukaryotic-specific’ systems, such as a complex cytoskeleton, may have originated prior to eukaryogenesis (the evolutionary process that gave rise to the Last Eukaryotic Common Ancestor (LECA)). Phylogenetic …
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Construction and initial characterization of yeast strains with Dyskeratosis congenita mutations
… mutations are well tolerated in the unicellular eukaryote Saccharomyces cerevisiae.
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Binding study of nickel complex to protein by equilibrium dialysis
… serum albumin which are typical proteins in eukaryote animals were chosen as the target materials when I studied whether this nickel complex could bind to proteins in the body. Nickel complex was labeled with Ni63 during the dialysis, and counted by a liquid scintillation counter.</p>
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