Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 56 for “"essential genes"”.
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Essential Genes And Their Role In Autism Spectrum Disorder
Essential genes (EGs) play central roles in fundamental cellular processes and are required for the survival of an organism. EGs are enriched for human disease genes and are under strong purifying selection. This intolerance to deleterious mutations, commonly observed haploinsufficiency and the …
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Characterisation of non-essential genes required for survival under conditions of DNA stress
… pombe (S. pombe) TopBP1 homologue that is essential for the initiation of DNA replication, DNA repair as well as the activation of DNA damage checkpoint signalling. We have modelled conditions of replication stress in the genetically tractable fission yeast, S. pombe using the hypomorphic …
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Construction and Use of a Transposon for Identification of Essential Genes in Mycobacteria
… TnQuoVadis, was constructed to identify essential genes as potential drug targets. This transposon has an outward-facing anhydrotetracycline (ATc)-inducible promoter at each end. A mutant with TnQuoVadis inserted upstream of an essential gene may display normal growth in the presence of …
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The organization, expression, function and evolution of some essential genes from the hyperthermophilic eubacterium thermotoga maritima
… and primer extension techniques. The tRNAgenes and the protein-encoding genes are co transcribed, except the 13 gene, which is transcribed separately. The following regulatory sequence elements were identified in this cloned fragment: five promoters (Pi and P2 in front of the first and …
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Probing the Mycobacterial Cell Envelope: CRISPR interference-mediated investigation of two essential genes in the galactan biosynthetic pathway
… highlights the need for the identification of essential cellular pathways in mycobacteria that can be targeted for novel drug development. A key feature of Mtb that confers intrinsic tolerance to many antibiotics is its complex cell envelope. The mycobacterial cell envelope is a dynamic …
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Analysis of Nitrogen starvation induced filamentous growth and characterization of putative essential genes in the human fungal pathogen, Candida albicans
… induzieren, und die Kontrolle der Morphogenese durch die Verfügbarkeit von Stickstoff wurde in dieser Arbeit detailliert untersucht. Ammonium ist für Hefepilze eine bevorzugte Stickstoffquelle, die über spezifische Transporter in die Zelle aufgenommen wird. In der vorliegenden Arbeit …
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Studies of programmed cell death in the nematode caenorhabditis elegans
… and engulfment-defective animals, and these genes are required for the B.al/rapaav cell death. In engulfment mutants the B.al/rapaav death process fails at a point after caspase activation, suggesting that the core cell-death pathway is necessary but not sufficient for this cell death. …
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Biases and Blind-Spots In Genome-Wide Crispr-Cas9 Knockout Screens
… genome-scale genetic perturbations to study essential genes, whose loss of function results in a severe fitness defect. There are two types of essential genes in a cell. Core essential genes are absolutely required for growth and proliferation in every cell type. On the other hand, …
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Inferring context-specific essentiality networks using large-scale CRISPR-KO screens
… DepMap and Project Score, revealed that a lot of genes are essential, i.e. required, in only a subset of cell lines. These context-essential genes offer insights into vulnerabilities of different cancer types and provide promising targets for personalized cancer therapies. However, the challenge …
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SMARCB1 Maintains Lineage Fidelity in Clear Cell Renal Cell Carcinoma
… factors have emerged as a promising class of essential genes in cancer. The best examples of leveraging this phenomenon in the clinic is targeting the androgen receptor and the oestrogen receptor in prostate and breast cancer respectively. Despite the success of these therapies, the mechanisms …
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High resolution alignment of the physical and genetic maps of the dpy-14 region of chromosome I in Caenorhabditis elegans
… physically and genetically. Lethal alleles of 15 essential genes, including dpy-14(e188ts), have been mapped near to or within the interval defined by unc-87 and the transposon insertion site dimorphism sPl. Much is known about the physical organization of this region, but the number and position …
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Development and validation of a CRISPR interference system for gene regulation in Campylobacter jejuni
… our ability to interrogate the function of essential and conditionally essential genes. This thesis aims to address this by developing and utilising a CRISPR-based interference system known as CRISPRi in C. jejuni to control gene expression and thereby investigate gene function. To achieve …
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Genome engineering and functional gene regulation tools for the study of malaria parasites
… The identification and characterization of essential functions for parasite survival are fundamental steps towards the creation of effective antimalarial therapies. In this work, we developed an integrated set of gene editing and functional gene regulation tools that enable the study of …
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Investigation of Proliferation Suppressors In Genetic Fitness Screens
… in collections of human cell lines can knock out genes at multiple loci, and have provided new insights into functional roles for independent genes. This method has launched massive efforts in looking across genetic backgrounds for context specific genetic vulnerabilities within cancer. Much of …
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Development and application of a general method for somatic cell genetics
… an efficient method for identifying the set of genes involved in a biological process. In microorganisms, powerful techniques enable systematic loss-of-function screens, which have yielded critical insights into the molecular basis of many fundamental cellular pathways. In human cells, however, …
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Identifying the Genetic Factors in Natural Genome Backgrounds That May Modulate Phenotypic Outcomes in C. elegans
Loss-of-function mutations in essential genes can lead to a severe phenotype characterized by reproductive stress or hindrance in development and survival. Whilst in some individuals these lethal mutations cause severe phenotypes, in others, phenotypic variability is observed. One of the factors …
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Development of Graphical Models and Statistical Physics Motivated Approaches to Genomic Investigations
<p>Identifying genes involved in disease pathology has been a goal of genomic research since the early days of the field. However, as technology improves and the body of research grows, we are faced with more questions than answers. Among these is the pressing matter of our incomplete understanding …
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Dominant Suppression of Early Innate Immune Mechanisms by Yersinia pestis
… offering an explanation as to why these genes were lost during the course of <italic>Y. pestis</italic> evolution. I also developed a negative selection screen termed transposon site hybridization: TraSH) to identify bacterial factors required for the two different phases of <italic>Y. …
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Modelling fitness and stability of G protein-coupled receptor variants
… and improve the understanding of GPCR gene essentiality and gene-disease links4. Recently, machine learning (ML) models have made significant advances in protein structure prediction5 and variant effect prediction6. However, use of ML models in high-stakes decisions is challenging as the …
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