Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"essential gene"”.
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Characterization of Murine Cytomegalovirus m142 and m143 Essential Gene Products
… upon the expression of a cascade of viral genes: immediate early (IE), early (E), and late (L). To date only three MCMV IE proteins have been characterized (IE1, IE2 and IE3). Our laboratory recently identified a novel IE gene region within the Hind III I region of MCMV. These genes, m142 …
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FLP-mediated conditional loss of an essential gene to facilitate complementation assays
Commonly, when it is desirable to replace an essential gene with an allelic series of mutated genes, or genes with altered expression patterns, the complementing constructs are introduced into heterozygous plants, followed by the selection of homozygous null segregants. To overcome this laborious …
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Exploration of Cell Cycle-Specific Essential Gene Functions in the Microbial Plant Chlamydomonas Reinhardtii
… followed a candidate strategy based on reverse genetics. These studies have a provided insights into plant cell cycle control, but are generally dependent upon sequence conservation between plant and opisthokont genes. This thesis presents work from an ongoing screen to identify critical …
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Identification of bptA (bbe16) as an Essential Gene for the Persistence of the Lyme Disease Spirochete, Borrelia Burgdorferi, in its Natural Tick Vector
… to B. burgdorferi's infectivity, pathogenesis, host range, and/or maintenance in its complex life cycle. Specifically, the microarray data provided a basis for formulating new testable hypotheses regarding the life cycle of B. burgdorferi. Recent advancements in borrelial genetics, some …
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A genetic and molecular analysis of the bli-4 locus of Caenorhabditis elegans, an essential gene encoding KEX2-like subtilisin-typw serine endoproteases
This thesis reports a characterization of genetic mutations and molecular structure of the bli-4 (I) gene of Caenorhabditis elegans. The bli-4locus had been previously defined by a single recessive mutation, e937, which disrupts the structure of adult-stage cuticle causing the formation of …
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Construction and Use of a Transposon for Identification of Essential Genes in Mycobacteria
… TnQuoVadis, was constructed to identify essential genes as potential drug targets. This transposon has an outward-facing anhydrotetracycline (ATc)-inducible promoter at each end. A mutant with TnQuoVadis inserted upstream of an essential gene may display normal growth in the presence of …
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Biochemical and molecular characterization of chemotaxis genes in Bacillus subtilis
The cheF gene, an essential gene for chemotaxis in B. subtilis was cloned, localized and characterized. This gene is contained in a 7.7 kb EcoR1 DNA fragment that was isolated from a $\lambda$ Charon 4A B. subtilis chromosomal DNA library. A 0.7 kb Pst 1 DNA fragment of this 7.7 kb EcoR1 fragment …
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Characterization of SEC14: A gene essential for secretion in Saccharomyces cerevisiae
SEC14, a Saccharomyces cerevisiae gene whose product is required for export of yeast secretory proteins from the Golgi complex, was cloned and characterized. SEC14 was shown to be an essential gene for vegetative growth. Nucleotide sequencing revealed SEC14 to be an intron-containing gene, which …
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Characterization of Drosophila Ctr1a: New Roles for Ctr1 Proteins and Copper in Physiology and Cell Signaling Pathways
<p>Copper is an essential trace element required by all aerobic organisms as a co-factor for enzymes involved in normal growth, development and physiology. Ctr1 proteins are members of a highly conserved family of copper importers responsible for copper uptake across the plasma membrane. Mice …
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Essential GPI Mannosyltransferases in Saccharomyces Cerevisiae and the Pathogenic Fungus Candida Albicans
… in vivo. I determined that CaSMP3 is also an essential gene, as I was unable to recover homozygous null smp3 mutants from C. albicans, an obligate diploid. I created a conditional strain in which expression of CaSMP3 was controlled by the CaMAL2 promoter, and found that depletion of CaSmp3p …
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Design of a Tetracycline Operon Inducible System for the Control of Vaccinia Virus Replication: Implications for Vaccine Development
… </em>operon to control transcription of VACV genes essential for virus growth. This would allow viral replication to be regulated through the addition or removal of tetracyclines. Seven VACV genes were tested in an attempt to control viral replication. For each gene a different recombinant was …
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The Cell-Essentiality of KAT7 in Acute Myeloid Leukemia
… identified KAT7 as an AML-specific cell-essential gene and therefore may represent a potential novel therapeutic target for AML. Here, I show that KAT7 loss leads to a rapid and dramatic global reduction in both H3K14ac and H4K12ac in association with reduced proliferation, increased …
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Analysis of Nitrogen starvation induced filamentous growth and characterization of putative essential genes in the human fungal pathogen, Candida albicans
… Candida albicans ist ein opportunistisch pathogener Hefepilz, der sowohl oberflächliche Infektionen der Schleimhaut als auch lebensbedrohliche systemische Infektionen hervorrufen kann. Obwohl die Fähigkeit von C.albicans Infektionen auszulösen weitgehend vom Immunstatus des Wirts abhängt, …
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Isolation and characterization of CEABF-1, the ABF-1 homolog in C. elegans
… lineage commitment, sex determination, neurogenesis, myogenesis, hematopoeisis and pancreatic development. The free-living nematode Caenorhabdits elegans is an important model organism. Genetics studies of a gene in nematodes help us to better understand the functioning of hornologs in more …
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BIOLOGICAL CHARACTERIZATION OF TWO PUTATIVE DNA METABOLISM ENZYMES IN DEINOCOCCUS RADIODURANS
… DNA during cell division. In archaea, herA is an essential gene that encodes an enzyme believed to be important for recombinational DNA repair. It is typically found in an operon with a gene that codes for a nuclease, nurA. Homologs of herA and nurA are found in a few bacterial genomes. In most …
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High-throughput genomic phenotyping
… of an organism, it has become expedient to generate methodologies to elucidate and characterize the function of all genes constituting the complete genetic makeup of an organism, whereby the knowledge of the genetic code may be for scientific and intellectual profit. This work consists of an …
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Discovery and characterization of Cas13b, a differentially regulated RNA-targeting CRISPR system
… Cas13b was characterized both biochemically and genetically, and found to be differentially regulated--inhibited by Csx27 in VI-B1 systems and enhanced by Csx28 in VI-B2 systems. RNA-targeting rules are critical to tool development, and so an E. coli essential gene screen was conducted and …
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Role of the Ajuba LIM Proteins in Epithelial Growth Regulation
… to the Drosophila model system where a single gene, CG11063 or djub, exists. The generation and analysis of Drosophila containing djub mutant loss-of-function alleles or depleted of dJub by RNAi identify djub as an essential gene required for normal development and a novel regulator of …
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LINKING CHROMATIN REMODELING TO MITOCHONDRIAL DYSFUNCTION: A ROLE FOR THE SMARCA5-MYC-TFAM AXIS IN HEPATOCELLULAR CARCINOMA
… In this study, a CRISPR/Cas9-based reverse genetic screen targeting novel mitochondrial regulators identified Smarca5 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 5), an ATP-dependent chromatin remodeler of the ISWI family, as essential …
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Mutational analysis of Hsp110 suggests an integral role for the chaperone in yeast prion propagation.
… molecular chaperone Hsp70. SSE1 and SSE2 are an essential gene pair and have been implicated in yeast cellular homeostasis. The Sse1 mutants disrupted cellular thermostability at 37oC and 39oC. Mutant G616D displayed osmoremedial temperature sensitivity at 37oC. Disruption to cell wall integrity …
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