Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 128 for “"erythroid"”.
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Repression Of Autophagy During Erythroid Development By Gfi1b
… mutants, which could explain ablation of the erythroid lineage and embryonic lethality in the mutant embryos.</p> <p>To investigate the role of GFI1B in regulating autophagy, I first performed an <em>in-silica</em> analysis of the genomic loci of several autophagy mediators (not previously …
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Study of Erythroid Antioxidant Depletion in Childhood Obesity
Obesity and overweight currently affects one third of children worldwide, and approximately 60% of cases present concomitant metabolic alterations that could trigger future complications. Among these alterations, insulin resistance has been identified as a forerunner state in the onset of …
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Stage-Specific Multiomic Analysis of Human Erythroid Differentiation
… Understanding the mechanisms of Epo-dependent erythroid differentiation is critical for identifying targets for the future development of therapeutics that increase RBC production and improve patient outcomes. Background: Erythropoiesis, the process of RBC (erythrocyte) production from …
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Determinants of GATA1-mediated gene regulation during erythroid maturation
… the 3D organization of the differentiating erythroid cell. In a second study, we identify a new feedback mechanism which facilitates the replacement of GATA2, a transcription factor expressed at earlier stages of hematopoiesis, by GATA1. We show that Fbw7, an ubiquitin ligase protein …
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GATA1 Occupancy Site Regulates Erythroid Cell Fitness through Pleiotropic Functions
<p>Understanding how non-coding variants influence cellular function remains a significant challenge in genetics, largely due to limited knowledge about regulatory sequence grammar, complex chromatin environments, and the transcriptional regulatory networks that link genotype to phenotype. In this …
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THE NON-ERYTHROID MEMBRANE SKELETON AND ITS ROLE IN MECHANOTRANSDUCTION
… skeleton in transducing forces to and from non-erythroid mammalian cells. This spectrin based membrane skeleton has been the focus of much study in the context of red blood cells, as it determines their mechanical properties due to the lack of an extended actin based cytoskeleton. In stark …
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The Role of Adenosine Signaling In Mature Erythrocytes and Erythroid Progenitors
… its significance and functional role in the erythroid lineage, particularly under stress conditions, are still unclear.</p> <p>My dissertation employing multi-disciplinary approaches including human studies, novel genetic tools, and sophisticated pharmacological studies coupled with …
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Finding lncRNAs in bone marrow and fetal liver erythroid progenitor cells in mice
Red blood cell development is crucial to the survival of all mammals and occurs primarily in the liver during embryogenesis and then in the bone marrow during adulthood. In spite of the different microenvironments of the liver and bone marrow, current research shows that the majority of …
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Purification and Characterization of a Methyl-DNA Binding Protein Complex from Primary Erythroid Cells
… methyl-DNA binding complex, termed the MeCPC (Erythroid Methyl Cytosine-binding Protein Complex), has been found to bind to the methylated, but not unmethylated, ρ-globin promoter and proximal transcribed region in nuclear extracts from definitive erythrocytes. This complex has a stronger …
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The Roles of Krüppel-Like Factor 1 (KLF1) in the Human Fetal Erythroid Compartment.
<p>Erythroid Krüppel-like factor (EKLF or KLF1) is a transcription factor with roles in embryonic and adult erythropoiesis. KLF1 knockout mouse embryos die due to severe anemia. Dominant human mutations in KLF1 can cause hereditary persistence of fetal hemoglobin. We show that KLF1 positively …
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Analysis of Mouse EKLF/KLF2 E9.5 Double Knockout: Yolk Sac Morphology and Embryonic Erythroid Maturation
… is implicated in the development of primitive erythroid and endothelial cells of the vasculature. Using light and electron microscopy, the yolk sacs and dorsal aortae from EKLF/KLF2 double knockout (KO) E9.5 (embryonic day 9.5) were examined to determine whether these KLFs have compensatory …
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Mechanisms of Erythroid Proliferation and Differentiation: Analysis of the Role of Erythropoietin Receptor in the Friend Virus Model
<p>Terminal erythroid differentiation is a precisely regulated process, in which mature red blood cells are generated from late erythroid progenitor cells. Regulation of cellular proliferation, survival and differentiation determines the fate of those cells. Extracellular signaling molecule, …
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Regulation of human 5-aminolevulinate synthase 2 expression during erythroid differentiation and its role in x-linked sideroblastic anaemia.
… processes including haemoglobin synthesis in erythroid cells, where the majority of the haem in the body is produced and utilised. Its synthesis requires tight regulation to prevent toxic levels of free haem from arising. 5-Aminolevulinate synthase 2 (ALAS2) is the first and rate limiting …
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Generation and Characterization of a Knock-In Allele of EKLF: Probing the in vivo Role of the Chromatin Remodeling Domain in Definitive Hematopoietic Cells
… zinc finger-encoding transacting factor EKLF, or erythroid Krüppel-like factor, binds key regulatory elements of many erythroid-specific genes, and is essential for definitive erythropoiesis. Mice lacking this factor die of anemia by E15.5 of gestation, failing to activate β-globin gene …
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Transcriptional divergence and conservation of human and mouse erythropoiesis
… sorted populations of human and mouse erythroid precursors from early to late erythroblasts. Induction and repression of major transcriptional regulators of erythropoiesis, as well as major erythroid-important proteins, are largely conserved between the species. In contrast, at a global …
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The Roles of Krüppel-like Transcription Factors KLF1 and KLF2 in Mouse Embryonic and Human Fetal Erythropoiesis
… of the silenced fetal gamma-globin gene in adult erythroid cells as a therapeutic strategy to alleviate the symptoms of beta-globin deficiencies have met with some success for the treatment of sickle cell anemia but not for beta-thalassemia. A better understanding of normal gamma-globin gene …
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The Role of AF9 and AF9-Mediated Protein Interactions in Hematopoiesis and Leukemogenesis
… of hematopoietic progenitor cells to the erythroid, myeloid and megakaryocytic lineages in the presence or absence of Af9. Based on previously published studies, we hypothesized that loss of Af9 would result in a loss of erythroid colony formation. Our results indicate no significant …
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Single Cell Proteogenomics Analysis of AML Samples Treated with Decitabine and Venetoclax Reveals Divergent Treatment Escape Mechanisms
… Three patients had monocytic shifts, one had an erythroid shift, and one had a mixed monocytic-erythroid shift. We did not always observe the outgrowth of genetic mutations at relapse. However, in one case, a patient showed an expansion of NRAS mutation accompanied by an erythroid shift. In …
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The roll of integrins in hematopoiesis
… cells at two developmental stages. In the erythroid lineage, I show that expansion of committed erythroid progenitors is regulated by growth factor and integrin-mediated signals in temporally distinct regimes. I establish a biologically relevant role for [alpha]401 but not [alpha]501 …
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