Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 15 of 15 for “"epitope-tag"”.
-
Studies on the structural organization of Golgi complex
… the observed kinetics of Golgi disassembly. An epitope tagging approach was used to delineate the importance of the above Golgi protein's cytoplasmic tail domain in Golgi targeting and retention. we found that the cytoplasmic tail could be lengthened considerably (3-4 fold) and SialyIT and GalT …
-
A Genetic-Proteomic Approach to Identify Cellular Components that Interact with HIV-1
… the cellular environment to its own advantage. However, to date, only a small group of cellular proteins have been shown to be required for viral propagation. In an effort to recover and identify those host proteins that interact in complex with the viral machinery, we have developed a …
-
The heteromeric 5-HT3A/B receptor:the effect of 5-HT3B subunits on receptor structure and ligand recognition
… was engineered to express a unique C-terminal epitope tag, together with six sequential histidine residues to facilitate nickel affinity purification. The 5-HT3 receptors, ectopically expressed in HEK293 cells, were solubilised, purified and decorated with antibodies to the subunit specific …
-
Action of Tyrosyl DNA Phosphodiesterase on 3'-Phosphoglycolate Terminated DNA Strand Breaks
… native enzyme. On cloning recombinant FLAG-tagged hTdpl into human expression vectors, we observed that the FLAG epitope tag did not show any evidence of affecting the specificity of the enzyme. Due to the many differences between bacterial and human cells, we cloned recombinant FLAG-tagged …
-
Next generation approaches toward engineering therapeutic proteases
… protein fusion that capitalizes on the p53 antagonist MDM2 as a detector of protease activity in addition to its utility as a counter-selection substrate. Using this system we successfully isolated OmpT variants that selectively cleave a designed site within our autoinhibited substrate. A …
-
Trim24 As An Oncogene In The Mammary Gland
… unable to assess. My project takes advantage of a new mouse model to understand the role of a proto-oncogenic, transcriptional co-regulator, TRIM24, in mammary gland development and disease.</p> <p>We previously reported an unknown binding partner and negative regulator of p53, Tripartite …
-
CDC45 Function Alters Cell Sensitivity to DNA Topoisomerase I Poisons
… to Top1T<sup>722</sup>A and our attempts to epitope tag Cdc45 and <em>cdc45-10</em>.</p> <p>The <em>cdc45-10</em> defects in origin firing and replication fork progression were characterized by isolating replication intermediates and resolving them using 2-D gel electrophoresis.</p> …
-
NANOGP1 as a Model to Study the Consequences of Gene Duplications on Human Pluripotency and Development
… gene editing in naïve PSCs, I introduced an epitope tag at the start of the predicted protein sequence, and this enabled me to demonstrate for the first time that endogenous NANOGP1 encodes an expressed protein. The ability to be translated into the stable protein raised the possibility that …
-
Functional analysis of tcf21 and tbx20 in zebrafish
… way to get around these problems is to express a tagged version of the transcription factor. The tag is recognizable by the same antibody; however, expressing the tagged transcription factor in this manner almost inevitably results in higher than normal levels of expression, leading to false …
-
Multiplexed Mapping of the Interactome of G Protein-Coupled Receptors and Receptor Activity-Modifying Proteins
… (Abs). We first prepared a library of dual epitope-tagged GPCR clones and complementary dual epitope-tagged RAMPs. We expressed each clone in the library in cultured cells to generate solubilized membranes harboring each GPCR alone, or together with each RAMP. We next tested >400 anti-GPCR …
-
Quantification and functional characterization of Sinorhizobium meliloti chemotaxis proteins
… in S. meliloti due to the fusion of common epitope tags to the C-terminus. The fusion of these tags promotes greater cellular abundance of many receptors including McpU. The fusion of charged residues to the C-terminus promotes a greater increase in McpU abundance thanthe addition of single …
-
Construction and analysis of novel controllable expression vectors for Bacillus subtilis
… The third vector allows addition of the c-Myc epitope tag (pHT10), and the remaining three vectors provide the purification tags His (pHT08) and Strep (pHT09 and pHT24). The versatility of all six vectors was verified by insertion of appropriate reporter genes and by demonstrating high level …
-
Characterisation of factors that regulate homologous recombination and antigenic variation in Trypanosoma brucei
… work demonstrated that it is possible to add an epitope tag for tandem affinity purification (TAP) to the N-terminus of RAD51 in both the bloodstream and procyclic stages of T. brucei without disrupting its function. Preliminary data suggest that TAP is potentially a feasible way of examining …
-
Novel approaches towards vaccine developments against porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus
… immune response against PCV2cap and the inserted epitope tag. Therefore, we evaluated the use of the non-pathogenic PCV1 wild-type (wt) virus and chimeric PCV1-2a vaccine virus (vs) to express four known B-cell epitopes of PRRSV. Peptide epitopes of PRRSV-VR2385, including GP2II (aa 40–51, …
-
Membrane Domain of Plant 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase: Targeting, Topology, and Function
… was unchanged. HMG1 membrane domain was tagged with a FLAG epitope to facilitate in vivo studies. Agrobacterium-mediated transformation was used to introduce the tagged hmg1 gene into two Nicotiana tabacum cell lines, BY-2 and KY-14. The slow growth kinetics of KY-14 prevented effective …