Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 63 for “"epithelial barrier"”.
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Immune activation at the lung epithelial barrier
… important role for structural cells, especially epithelial cells, in allergic asthma. Lung epithelial cells express pattern recognition receptors (such as TLR4) and respond to allergens by producing DC-instructing factors. Chapter 5 demonstrates that exposure of lung epithelial cells to HDM leads …
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Host-Pathogen Interactions at the Intestinal Epithelial Barrier
… of the interactions between the intestinal epithelial barrier and the intracellular apicomplexan Eimeria spp., both in vivo and in vitro. Initially, conventional in vivo studies using genetically modified animals were used to investigate the contribution of innate lymphoid cells (ILCs) to …
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Staphylococcus aureus Exoproteins on Nasal Epithelial Barrier in Chronic Rhinosinusitis
… their effect on in-vitro models of the nasal epithelial barrier. As a result of this study we found S. aureus biofilm exoproteins disrupt the mucosal barrier structure in a time- and dose- dependent manner and are toxic to the barrier. This damage to the mucosal barrier is thought to play an …
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Role of matriptase in the regulation of epithelial barrier permeability studied using MDCK cells
… the!integrity!of!epithelial!barriers.!However,!the!molecular!mechanisms!underlying! the!role!of!matriptase!are!unknown.! To! study! these!mechanisms,! two!variants!of!MadinNDarby!canine!kidney!(MDCK)! cells!were! used,! together!with! the! “calciumNswitch”!model! …
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NS1 of H5N1 Interacts with SAP-97 in a PDZ-dependent Manner to Disrupt Epithelial Barrier Integrity
… of NS1 and SAP-97 results in the loss of epithelial barrier function. This mechanism helps to explain why the two disease states, pulmonary edema and diarrhea, in which epithelial barrier is compromised, are both common in human H5N1 infection.</p>
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Toxicological effects of metal oxide nanoparticles in combination with microbial components and inflammatory mediators on intestinal epithelial cells
… Research has shown that maintenance of epithelial barrier plays an important role in mucosal homeostasis. Intestinal epithelial cells (IECs) play a critical role in formation and maintenance of the intestinal epithelial barrier which functions as a protective barrier from microbial …
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Identification of the molecular pathways that mediate cytokine induced immunopathology of the gut epithelium
… are required for the killing of human intestinal epithelial cells (Chapter-I) by this cytokine combination. A human kinome RNAi screen and a drug repurposing screen identified the Janus kinases (JAKs) JAK1 and JAK2 as the major kinases required for IFN-γ + TNF-α dependent synergistic cell death. …
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The Tissue Specific Role of Endosome Associated Protein SNX27 in Colonic Inflammation and Tumorigenesis
… intestinal disorders remains to be unexplored. Epithelial barrier integrity, regulated by junction proteins, is critical for the maintenance of intestinal tissue homeostasis. Weakening of the intestinal barrier thus promotes susceptibility towards intestinal disorders, such as IBD and CRC. Many …
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YAP/TAZ REGULATES ALVEOLAR REGENERATION AND RESOLUTION OF LUNG INFLAMMATION
… for recovery from these insults to rebuild the epithelial barrier and restore pulmonary functions. Here, we show that sublethal infection of mice with Streptococcus pneumonia, the most common pathogen of community-acquired pneumonia, led to exclusive damage in lung alveoli, followed by alveolar …
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Effect of Diabetes Mellitus on Ocular Surface Tight Junctions and Glycocalyx
… diabetes mullites include impairment of corneal epithelial barrier function, conjunctival defects, increased incidence of corneal and conjunctival infections and higher prevalence of dry eye disease. Impairment of tight junctions and glycocalyx, which are critical for ocular surface barrier …
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Development and Characterisation of an Immunocompetent Three-Dimensional Oral Mucosal Infection Model
… develop into an IC3DOMM, due to its superior epithelial barrier, and ability to upregulate both IL-6 and IL-8 in response to infection, when compared to the to the POK based P3DOMM. Model-incorporated THP-1 cells increased in size and altered morphology, suggesting differentiation into …
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The damaged mucosal barrier in intestinal inflammation: interaction with therapeutic bacteria and roles for metallothioneins as immunomodulators
… studies underscore the importance of the damaged epithelial barrier and the dysregulated innate immune system in the pathogenesis of IBD. These two components cause defects in the interaction of the host with the bacterial flora. The epithelium must maintain a physical barrier between the lumen …
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Role of Occludin in the Regulation of Epithelial Tight Junctions
… (TJ) constitute the primary component of epithelial barrier function, a disruption of which is involved in the pathogenesis of many gastrointestinal, pulmonary and renal diseases. Occludin is the major transmembrane protein of TJ, a deletion of which leads to a complex phenotype including …
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Uptake and transport of orally-deliverable drugs across caco-2 cell monolayers:the effect of lipid formulations
… on drug uptake and transport across in vitro epithelial barrier models. Liposomes composed of phosphatidylcholine (PC) or distearoyl phosphatidylcholine (DSPC) and cholesterol (0, 4, 8, 16 µM) were prepared and optimised in terms of drug loading using the hand-shaking method (Bangham et al., …
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Mechanisms of virus infection of human airway epithelium cultured at an air-liquid interface (HAE-ALI)
ABSTRACT Polarized human airway epithelial (HAE) cultures are instrumental for studying respiratory tract diseases and gene delivery of airways. Primary epithelial cells isolated from tracheobronchial airways, grown in transwell at an air-liquid interface (ALI), undergo media-induced mucociliary …
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Developing bioelectronic sensors for 3D in vitro models at the air liquid interface: Innovative technology for biomedical and respiratory research.
… are characterized by aberrant immune and epithelial barrier function, leading to respiratory inflammation and tissue damage. Despite substantial progress in identifying therapeutic targets and drug candidates, the efficacy of novel treatments in clinical trials remains low. One of the …
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The Role of Bacterial Amyloids In Regulating Gastrointestinal Homeostasis
… first phase of our studies, utilizing intestinal epithelial cells polarized on semi-permeable tissue culture inserts (Transwells®), we observed that the recognition of curli fibers on Salmonella enterica serovar Typhimurium by intestinal epithelial cells led to the augmentation of the intestinal …
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Protective Role of Mesenchymal MyD88 Signaling Under Homeostasis and Initiation of Inflammation in the Colon
… and the host is reported to contribute to epithelial barrier restoration via repositioning of mesenchymal stromal cells producing COX-2/PGE2 during injury. However, whether MyD88 signaling in mesenchymal cells is involved in this process is not known. Moreover, mesenchymal cell MyD88 …
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Identification of novel genetic determinants in the high prevalence early-onset inflammatory bowel disease population in Scotland
… (p=0.03 OR 2.4 (1.05–5.6)). Null alleles of the epithelial barrier protein FLG have no important effect on IBD susceptibility (p>0.4), but contribute to the high prevalence of atopy, notably co-existent eczema and food allergy (p=0.0003 OR 3.3 (1.7–6.6) and p=0.0001 OR 4.5 (2.0–10.0), …
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On Microvascular Inflammation and Intestinal Leakage in Radiation Enteropathy
… leukocyte and platelet recruitment as well as epithelial barrier dysfunction, are considered to constitute key components in the pathophysiology of radiation-induced enteropathy. The aim of this thesis was to investigate the basic mechanisms behind leukocyte-platelet-endothelial cell …
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